Details:

Alternative Title:
BMC Microbiol
Personal Author:
Hendriksen, Rene S ; Hyytia-Trees, Eija ; Pulsrikarn, Chaiwat ; Pornruangwong, Srirat ; Chaichana, Phattharaporn ; ... More +
Hendriksen, Rene S ; Hyytia-Trees, Eija ; Pulsrikarn, Chaiwat ; Pornruangwong, Srirat ; Chaichana, Phattharaporn ; Svendsen, Christina Aaby ; Ahmed, Rafiq ; Mikoleit, Matthew Less -
Description:
Background

Bacteremia due to Salmonella spp. is a life-threatening condition and is commonly associated with immune compromise. A 2009 observational study estimated risk factors for the ten most common non-typhoidal Salmonella (NTS) serovars isolated from Thai patients between 2002–2007. In this study, 60.8% of Salmonella enterica serovar Enteritidis isolates (n = 1517) were recovered from blood specimens and infection with Salmonella serovar Enteritidis was a statistically significant risk factor for bacteremia when compared to other NTS serovars. Based on this information, we characterized a subset of isolates collected in 2008 to determine if specific clones were recovered from blood or stool specimens at a higher rate. Twenty blood isolates and 20 stool isolates were selected for antimicrobial resistance testing (MIC), phage typing, PFGE, and MLVA.

Result

Eight antibiogrammes, seven MLVA types, 14 XbaI/BlnI PFGE pattern combinations, and 11 phage types were observed indicating considerable diversity among the 40 isolates characterized. Composite analysis based on PFGE and MLVA data revealed 22 genotypes. Seven of the genotypes containing two or more isolates were from both stool and blood specimens originating from various months and zones. Additionally, those genotypes were all further discriminated by phage type and/or antibiogramme. Ninety percent of the isolates were ciprofloxacin resistant.

Conclusions

The increased percentage of bloodstream infections as described in the 2009 observational study could not be attributed to a single clone. Future efforts should focus on assessing the immune status of bacteriaemic patients and identifying prevention and control measures, including attribution studies characterizing non-clinical (animal, food, and environmental) isolates.


Subjects:
[+]
Adolescent Adult Aged Aged, 80 And Over Anti-Bacterial Agents Bacteremia Bacteriophage Typing Blood Child Child, Preschool Cluster Analysis Electrophoresis, Gel, Pulsed-Field Feces Female Genotype Humans Infant Male Microbial Sensitivity Tests Middle Aged Minisatellite Repeats Molecular Typing Phenotype Salmonella Enteritidis Salmonella Infections Young Adult
Source:
BMC Microbiol. 2012; 12:92.
Document Type:
Journal Article
Place as Subject:
Thailand
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:5d16ef387ab9d77a59d2258bb82a1a8b96961bf2d4fa993611f70ad407efff7a
Download URL:
https://stacks.cdc.gov/view/cdc/12427/cdc_12427_DS1.pdf
File Type:

Supporting Files

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  	1471-2180-12-92-1.jpg	jpeg
  	1471-2180-12-92.nxml	txt
  	license.txt	txt

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