Peptide Amphiphile Mediated Co-assembly for Nanoplasmonic Sensing

Details

• Alternative Title:
  Angew Chem Int Ed Engl
• Personal Author:
  Jin, Zhicheng ; Li, Yi ; Li, Ke ; Zhou, Jiajing ; Yeung, Justin ; Ling, Chuxuan ; Yim, Wonjun ; He, Tengyu ; Cheng, Yong ; Xu, Ming ; Creyer, Matthew N. ; Chang, Yu-Ci ; Fajtová, Pavla ; Retout, Maurice ; Qi, Baiyan ; Li, Shuzhou ; O’Donoghue, Anthony J. ; Jokerst, Jesse V.
• Description:
  Aromatic interactions are commonly involved in the assembly of naturally occurring building blocks, and these interactions can be replicated in an artificial setting to produce functional materials. Here we describe a colorimetric biosensor using co-assembly experiments with plasmonic gold and surfactant-like peptides (SLPs) spanning a wide range of aromatic residues, polar stretches, and interfacial affinities. The SLPs programmed in DDD-(ZZ)| -FFPC self-assemble into higher-order structures in response to a protease and subsequently modulate the colloidal dispersity of gold leading to a colorimetric readout. Results show the strong aggregation propensity of the FFPC tail without polar DDD head. The SLPs were specific to the target protease, i.e., M| , a biomarker for SARS-CoV-2. This system is a simple and visual tool that senses M| in phosphate buffer, exhaled breath condensate, and saliva with detection limits of 15.7, 20.8, and 26.1 nM, respectively. These results may have value in designing other protease testing methods.
• Subjects:
  Aromatic Interactions Article Colorimetric Test COVID-19 Endopeptidases Gold Humans Main Protease Peptide Amphiphile Peptide Hydrolases Peptides Saliva SARS-CoV-2 Surface-Active Agents

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• Source:
  Angew Chem Int Ed Engl. 62(4):e202214394
• Pubmed ID:
  36409652
• Pubmed Central ID:
  PMC9852014
• Document Type:
  Journal Article
• Funding:
  R21 DE029917/DE/NIDCR NIH HHSUnited States/ ; R21 AI157957/AI/NIAID NIH HHSUnited States/ ; S10 OD023555/CD/ODCDC CDC HHSUnited States/ ; R21 AG065776/AG/NIA NIH HHSUnited States/ ; R01 DE031114/DE/NIDCR NIH HHSUnited States/
• Volume:
  62
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:cf843aa58af50aff67ecfbe873e76641981553fc589430b29fd8e274dd88baa9
• Download URL:
  https://stacks.cdc.gov/view/cdc/123836/cdc_123836_DS1.pdf
• File Type:
  [PDF - 1.69 MB ]