Details:

Alternative Title:
PLoS One
Personal Author:
Mao, Lili ; Wang, Jun ; DeGrado, William F. ; Inouye, Masayori
Mao, Lili ; Wang, Jun ; DeGrado, William F. ; Inouye, Masayori Less -
Description:
We developed a novel drug screening system for anti-influenza A virus by targeting the M2 proton channel. In the SPP (Single Protein Production) system, E. coli cell growth occurs only in the presence of effective M2 channel inhibitors, and thus simple measurement of cell growth was used as readouts for drug screening. Two potential inhibitors for M2 (V27A) mutant were verified using this method, which inhibit both the mutant and wild-type M2 channels.
Subject:
[+]
Antiviral Agents
Biology
Drug Evaluation, Preclinical
Drug Resistance, Viral
Escherichia Coli
Gene Expression Regulation, Bacterial
High-Throughput Screening Assays
Humans
Influenza, Human
Influenza A Virus
Medicine
Mutation
Recombinant Proteins
Research Article
Viral Matrix Proteins

Source:
PLoS One. 2013; 8(1).
Document Type:
Journal Article
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:333e3e362b19eb7a83de5324b67168e14a75a25f3cffcadf03f4419fa071131f
File Type:

Supporting Files

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