An Assay Suitable for High Throughput Screening of Anti-Influenza Drugs

Mao, Lili; Wang, Jun; DeGrado, William F.; Inouye, Masayori

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An Assay Suitable for High Throughput Screening of Anti-Influenza Drugs

Supporting Files Public Domain

Jan 10 2013
By Mao, Lili ; Wang, Jun ; DeGrado, William F. ; ...

File Language:

English

Details

Alternative Title:

PLoS One
Personal Author:

Mao, Lili ; Wang, Jun ; DeGrado, William F. ; Inouye, Masayori
Description:

We developed a novel drug screening system for anti-influenza A virus by targeting the M2 proton channel. In the SPP (Single Protein Production) system, E. coli cell growth occurs only in the presence of effective M2 channel inhibitors, and thus simple measurement of cell growth was used as readouts for drug screening. Two potential inhibitors for M2 (V27A) mutant were verified using this method, which inhibit both the mutant and wild-type M2 channels.
Subjects:

Antiviral Agents Drug Evaluation, Preclinical Drug Resistance, Viral Escherichia Coli Gene Expression Regulation, Bacterial High-Throughput Screening Assays Humans Influenza, Human Influenza A Virus Mutation Recombinant Proteins Viral Matrix Proteins
Source:

PLoS One. 2013; 8(1).
Document Type:

Journal Article
Volume:

8
Issue:

1
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:333e3e362b19eb7a83de5324b67168e14a75a25f3cffcadf03f4419fa071131f
Download URL:

https://stacks.cdc.gov/view/cdc/12192/cdc_12192_DS1.pdf
File Type:

[PDF - 374.51 KB ]

File Language:

English

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