A Novel Comprehensive Efficacy Test for Textiles Intended for Use in the Healthcare Setting

Details

• Alternative Title:
  J Microbiol Methods
• Personal Author:
  Nicoloro, Jennifer M. ; Wen, Jianchuan ; Queiroz, Samantha ; Sun, Yuyu ; Goodyear, Nancy
• Description:
  Soft surfaces, including textiles are found throughout healthcare settings. Pathogens can survive for long periods of time on textiles, and can be transferred to and from the skin. Antimicrobial fabrics are used as an engineering control to prevent infection. Efficacy testing standards have limitations, including single microorganism challenges, multiple fabric plies tested, and lengthy contact times. We developed a novel method that better models in-use conditions through testing standardized mixtures of pathogens and normal skin microorganisms, artificial soils, and a 15-min contact time. Reproducible growth of all microorganisms from frozen stocks was achieved using this method. A novel rechargeable, monitorable N-halamine cotton cellulose fabric, containing 5885 ± 98 ppm of active chlorine, was evaluated with the new method using PBS, artificial sweat, and artificial sweat plus 5% serum as soil. Pathogens tested included Acinetobacter baumannii, Candida albicans, Escherichia coli, vancomycin-resistant Enterococcus faecalis, methicillin-resistant Staphylococcus aureus, methicillin-susceptible Staphylococcus aureus, and Pseudomonas aeruginosa. Each was tested singly and in the presence of a representative normal skin flora mixture, including: Acinetobacter lwoffii, Corynebacterium striatum, Micrococcus luteus, and Staphylococcus epidermidis. When tested singly, all microorganisms were reduced by 3.00 log| or greater, regardless of artificial soil. In mixture, 4.00 log| or greater reductions were achieved for all microorganisms. These results suggest that the novel testing method can be used to provide more comprehensive and realistic efficacy information for antimicrobial textiles intended for use in healthcare. Furthermore, the N-halamine fabric demonstrated efficacy against multiple pathogens, singly and in mixtures, regardless of the presence of artificial soils.
• Subjects:
  Anti-Bacterial Agents Anti-Infective Agents Antimicrobial Efficacy Testing Antimicrobial Fabric Article Bacteria Cell Culture Techniques Delivery Of Health Care Healthcare-Associated Infections Microbial Sensitivity Tests Soil Soil Microbiology Textiles

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• Source:
  J Microbiol Methods. 173:105937
• Pubmed ID:
  32387116
• Pubmed Central ID:
  PMC9453850
• Document Type:
  Journal Article
• Funding:
  R21 OH011406/OH/NIOSH CDC HHSUnited States/
• Volume:
  173
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:9a2ff9ce15decb8b224a62bac32f5a692b98867affbd494b2cf97bc1008dd168
• Download URL:
  https://stacks.cdc.gov/view/cdc/121080/cdc_121080_DS1.pdf
• File Type:
  [PDF - 956.19 KB ]