Comparison of Mycobacterium tuberculosis complex Yield and Contamination Rates using Lowenstein-Jensen with and without Antibiotics in Western Kenya

Details

• Alternative Title:
  J Med Sci Clin Res
• Personal Author:
  Okumu, A ; McCarthy, K ; Orwa, J ; Williamson, J ; Musau, S ; Alexander, H ; Cavanaugh, S ; Modi, S ; Cain, K
• Description:
  Background:
  
  While molecular methods have been recently endorsed for diagnosis of tuberculosis (TB), mycobacterial culture remains the gold standard. Lowenstein-Jensen (LJ) is often used for the cultivation of Mycobacterium tuberculosis complex (MTBC); however contamination often renders a subset of cultures useless. We compared the MTBC yield and contamination rate of processed sputum inoculated on LJ with antibiotics (LJ PACT) to LJ without antibiotics (LJ).
  
  Methodology:
  
  Sputum samples were obtained from people living with HIV enrolled in a TB screening study in western Kenya, processed using NALC/NaOH-Na citrate, then inoculated on LJ PACT and LJ media. Cultures were evaluated weekly with growth identified as acid-fast bacilli by Ziehl-Neelsen bright-field microscopy. MTBC and nontuberculous mycobacteria (NTM) were identified by immunochromatographic and line probe assays.
  
  Results:
  
  A total of 700 sputum samples were cultured on both LJ PACT and LJ between March and June 2012. Of those cultured on LJ PACT, 29 (4.1%) grew MTBC, 613 (87.6%) were negative, 12 (1.7%) grew NTM, and 46 (6.6%) were contaminated; on LJ, 28 (4%) grew MTBC, 553 (79%) were negative, 9 (1.3%) grew NTM, and 110 (15.7%) were contaminated. The difference in contamination on LJ PACT and LJ was statistically significant (p<0.0001), while the difference in MTBC growth was not (p=0.566).
  
  
• Subjects:
  Article Cross-sectional Studies Culture Techniques Diagnosis Kenya Lowenstein-Jensen Nontuberculous Mycobacteria Pulmonary Tuberculosis
• Source:
  J Med Sci Clin Res. 5(8)
• Pubmed ID:
  35979512
• Pubmed Central ID:
  PMC9380436
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Place as Subject:
  Kenya
• Volume:
  5
• Issue:
  8
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:21663068948dbf84de11bc4a44ca2ba17e968495129b90e73b937fba2db3f819
• Download URL:
  https://stacks.cdc.gov/view/cdc/120421/cdc_120421_DS1.pdf
• File Type:
  [PDF - 77.57 KB ]