Molecular Surveillance of Rotavirus Infection in the Democratic Republic of the Congo August 2009 to June 2012

Details

• Alternative Title:
  Pediatr Infect Dis J
• Personal Author:
  Pukuta, Elizabeth S. ; Esona, Mathew D. ; Nkongolo, Adolphe ; Seheri, Mapaseka ; Makasi, Mingiele ; Nyembwe, Michel ; Mondonge, Vital ; Dahl, Benjamin A. ; Mphahlele, M. Jeffrey ; Cavallaro, Kathleen ; Gentsch, Jon ; Bowen, Michael D. ; Waku-Kouomou, Diane ; Muyembe, Jean-Jacques
  More +
• Corporate Authors:
  The SURVAC Working Group
• Description:
  Background: ; Rotavirus is a major cause of severe diarrhea worldwide. It causes 453,000 deaths in children annually. In the Democratic Republic of the Congo, sentinel site surveillance of rotavirus gastroenteritis started in 2009 and aimed to document burden of rotavirus diarrhea and identify circulating rotavirus genotypes. ; Methods: ; Between August 2009 to June 2012, stool samples were collected in Kinshasa and Lubumbashi, from children <5 years of age who met the WHO case definition for rotavirus gastroenteritis. Rotavirus antigen detection was performed using an enzyme immunoassay technique and rotavirus strains were characterized using a multiplex reverse transcription polymerase chain reaction assay. ; Results: ; During the study period, 1614 stool samples were screened for rotavirus by enzyme immunoassay and 990 (61%) were positive. Of these, the genotype was determined in 330 (33%) samples. The most common genotypes found in the samples analyzed were G1P8 in 2009 (28%) and 2012 (33%), G2P4 (33%) in 2010 and G2P6 (28%) in 2011. Uncommon strains like G8P6 (5%), G6P6 (5%), G12P6 (3%), G12P8 (3%) and G8P8 (2%) were also detected. ; Conclusions: ; In Democratic Republic of the Congo, 61% of the diarrhea in children in <5 years of age was caused by rotavirus infection and a variety of rotavirus genotypes were detected. Implementation of rotavirus genotyping at the national level has improved the timely identification of rotavirus strains. These results will help decision makers in Democratic Republic of the Congo plan the implementation of a rotavirus vaccination program.
• Subjects:
  • Child, Preschool
  • Democratic Republic of the Congo
  • Diarrhea
  • Feces
  • Gastroenteritis
  • Humans
  • Infant
  • Infant, Newborn
  • Molecular Epidemiology
  • Rotavirus
  More +
• Keywords:
  • pediatric
  • rotavirus
  • surveillance
  • Democratic Republic of the Congo
• Source:
  Pediatr Infect Dis J. 33(4):355-359
• Pubmed ID:
  24637513
• Pubmed Central ID:
  PMC9241998
• Document Type:
  Journal Article
• Funding:
  001/WHO_/World Health OrganizationInternational/ ; CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  33
• Issue:
  4
• Download URL:
  https://stacks.cdc.gov/view/cdc/119331/cdc_119331_DS1.pdf
• File Type:
  [PDF - 453.52 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:f020c4534f94df72b62bf0ce33a7de52473346a53ac0989fbb7f29d4f530ad35