Analysis of the reiteration regions (R1 to R5) of varicella-zoster virus

Details

• Alternative Title:
  Virology
• Personal Author:
  Jensen, Nancy J. ; Depledge, Daniel P. ; Ng, Terry F. ; Quinlivan, Mark ; Radford, Kay W. ; Folster, Jennifer ; Tseng, Hung-Fu ; LaRussa, Philip ; Jacobsen, Steven J. ; Breuer, Judith ; Schmid, D. Scott
• Description:
  The varicella-zoster virus (VZV) genome, comprises both unique and repeated regions. The genome also includes reiteration regions, designated R1 to R5, which are tandemly repeating sequences termed elements. These regions represent an understudied feature of the VZV genome. The R4 region is duplicated, with one copy in the internal repeat short (IRs) which we designated R4A and a second copy in the terminal repeat short (TRs) termed R4B. We developed primers to amplify and Sanger sequence these regions, including independent amplification of both R4 regions. Reiteration regions from >80 cases of PCR-confirmed shingles were sequenced and analyzed. Complete genome sequences for the remaining portions of these viruses were determined using Illumina MiSeq. We identified 28 elements not previously reported, including at least one element for each R region. Length heterogeneity was substantial in R3, R4A and R4B. Length heterogeneity between the two copies of R4 was common.
• Subjects:
  DNA, Viral Genome, Viral Herpesvirus 3, Human Herpes Zoster Humans Polymerase Chain Reaction Protein Binding Tandem Repeat Sequences Viral Proteins
• Source:
  Virology. 546:38-50
• Pubmed ID:
  32452416
• Pubmed Central ID:
  PMC9169507
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/ ; G0700814/MRC_/Medical Research CouncilUnited Kingdom/
• Volume:
  546
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:1d9dc0cf0b525138f1ef4af731904c722dae2a830d6578abadacd48f12d26b1b
• Download URL:
  https://stacks.cdc.gov/view/cdc/119227/cdc_119227_DS1.pdf
• File Type:
  [PDF - 3.47 MB ]