Biofilms of Black Tooth Stains: PCR Analysis Reveals Presence of Streptococcus mutans

COSTA, Marília Teixeira; DORTA, Miriam Leandro; RIBEIRO-DIAS, Fátima; PIMENTA, Fabiana Cristina

doi:10.1590/s0103-64402012000500014

i

Biofilms of Black Tooth Stains: PCR Analysis Reveals Presence of Streptococcus mutans

Supporting Files

2012
By COSTA, Marília Teixeira ; DORTA, Miriam Leandro ; RIBEIRO-DIAS, Fátima ; ...

File Language:

English

Details

Alternative Title:

Braz Dent J
Personal Author:

COSTA, Marília Teixeira ; DORTA, Miriam Leandro ; RIBEIRO-DIAS, Fátima ; PIMENTA, Fabiana Cristina
Description:

This study investigated the presence of the black-pigmented bacteria Prevotella nigrescens and Prevotella intermedia, the non-black-pigmented bacteria Actinomyces spp and particularly the cariogenic pathogen Streptococcus mutans in the dental biofilms of patients with or without black extrinsic tooth stains, using the multiplex polymerase chain reaction (PCR) technique. Analysis of the dental biofilms of patients with (n=26) or without (n=26) black tooth stains was performed using duplex PCR for the 16S ribosomal RNA gene (P. nigrescens, P. intermedia, Actinomyces spp) and glucosyltransferase-I gene for S. mutans. P. nigrescens and S. mutans were the most frequent bacteria detected in both groups. The least frequently detected were P. intermedia and Actinomyces spp. The similar bacterial composition of dental biofilms of black tooth stains and healthy tooth surfaces indicates that black tooth stains are not free of cariogenic bacteria.
Subjects:

Actinomyces Adolescent Adult Biofilms Child Dental Plaque Female Humans Male Middle Aged Multiplex Polymerase Chain Reaction Prevotella Intermedia Prevotella Nigrescens RNA, Ribosomal, 16S Streptococcus Mutans Tooth Discoloration
Keywords:

Black-pigmented Bacteria Black Tooth Stain Dental Caries Mutans Streptococci PCR
Source:

Braz Dent J. 23(5):555-558
Pubmed ID:

23306233
Pubmed Central ID:

PMC9121365
Document Type:

Journal Article
Funding:

CC999999/ImCDC/Intramural CDC HHSUnited States/
Volume:

23
Issue:

5
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha-512:5cfdee3322556c924428ade1070af837d740d0aaca9eb4e2f229b42cfae891dfb530c31e4bacff317003a02fe346ed508996759fb108109ad64f86d2985f2715
Download URL:

https://stacks.cdc.gov/view/cdc/117652/cdc_117652_DS1.pdf
File Type:

[PDF - 135.18 KB ]

File Language:

English

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