Measurements of human herpesvirus 8 viral load in blood before and after leukoreduction filtration

Details

• Alternative Title:
  Transfusion
• Personal Author:
  Dollard, Sheila C. ; Roback, John D. ; Gunthel, Clifford ; Amin, Minal M. ; Barclay, Sheilagh ; Patrick, Ericka ; Kuehnert, Matthew J.
• Description:
  BACKGROUND:
  
  Human herpesvirus 8 (HHV-8) is likely transmitted through blood transfusion in high-prevalence areas. The efficacy of leukoreduction filtration for reducing HHV-8 in blood has not been reported.
  
  STUDY DESIGN AND METHODS:
  
  Blood was drawn from 45 human immunodeficiency virus–positive men either with Kaposi’s sarcoma (KS; n = 21) or without KS (n = 24) and subject to leukoreduction filtration. HHV-8 viral load was measured in plasma and in blood before and after filtration.
  
  RESULTS:
  
  Twelve subjects, all with KS, had detectable HHV-8 viremia before filtration with viral loads of 102 to 105 copies/mL (mean, 3 × 104 copies/mL). After filtration, seven of 12 subjects no longer had detectable HHV-8 in their blood, and five of 12 subjects had detectable HHV-8 that was 90% reduced on average from prefiltration levels. The presence of HHV-8 in the blood after filtration was strongly associated with prefiltration viral loads greater than 1000 copies/mL and the presence of cell-free virus in plasma. None of the subjects without KS had detectable levels of HHV-8 virus in blood before or after filtration.
  
  CONCLUSION:
  
  Cell-associated HHV-8 appeared to be effectively removed by leukoreduction filtration. Cell-free HHV-8 was present in 42% of subjects as 1% to 20% of the total virus which was not removed by filtration.
  
  
• Subjects:
  Article DNA, Viral Herpesvirus 8, Human Humans Leukocyte Reduction Procedures Male Viral Load Viremia
• Source:
  Transfusion. 53(10):2164-2167
• Pubmed ID:
  23362994
• Pubmed Central ID:
  PMC8721744
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  53
• Issue:
  10
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:64f9a6af5cdbf7f9119842be1c9b133291f726e4d7786fc0ca057ebf5feb7ea4
• Download URL:
  https://stacks.cdc.gov/view/cdc/113054/cdc_113054_DS1.pdf
• File Type:
  [PDF - 315.43 KB ]