Determination of Catechins and Caffeine in Camillia sinensis Raw Materials, Extracts, and Dietary Supplements by HPLC-UV: Single-Laboratory Validation

Details

• Alternative Title:
  J AOAC Int
• Personal Author:
  Roman, Mark C. ; Hildreth, Jana ; Bannister, Silvia
• Description:
  A rapid method has been developed to quantify seven catechins and caffeine in green tea (Camillia sinensis) raw material and powdered extract, and dietary supplements containing green tea extract. The method utilizes RP HPLC with a phenyl-based stationary phase and gradient elution. Detection is by UV absorbance. The total run time, including column re-equilibration, is 13 min. Single-laboratory validation (SLV) has been performed on the method to determine the repeatability, accuracy, selectivity, LOD, LOQ, ruggedness, and linearity for (+)-catechin, (-)-epicatechin, (-)-epicatechin gallate, (-)-epigallocatechin, (-)-gallocatechin gallate, (-)-epigallocatechin gallate, and (+)-gallocatechin, as well as caffeine. Repeatability precision and recovery results met AOAC guidelines for SLV studies for all catechins and caffeine down to a level of approximately 20 mg/g. Finished products containing high concentrations of minerals require the use of EDTA to prevent decomposition of the catechins.
• Subjects:
  Article Caffeine Camellia Sinensis Catechin Chromatography, High Pressure Liquid Reproducibility Of Results
• Source:
  J AOAC Int. 96(5):933-941
• Pubmed ID:
  24282928
• Pubmed Central ID:
  PMC4115333
• Document Type:
  Journal Article
• Funding:
  223-01-2464/NoneNone/ ; 223-01-2464/CD/ODCDC CDC HHSUnited States/
• Volume:
  96
• Issue:
  5
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:d884821189f7af8b3a9a0edc9baccbe75822f39a821fc5195ee0537b34b437fd
• Download URL:
  https://stacks.cdc.gov/view/cdc/110933/cdc_110933_DS1.pdf
• File Type:
  [PDF - 541.67 KB ]