Details:

Alternative Title:
J AOAC Int
Personal Author:
Roman, Mark C. ; Hildreth, Jana ; Bannister, Silvia
Roman, Mark C. ; Hildreth, Jana ; Bannister, Silvia Less -
Description:
A rapid method has been developed to quantify seven catechins and caffeine in green tea (Camillia sinensis) raw material and powdered extract, and dietary supplements containing green tea extract. The method utilizes RP HPLC with a phenyl-based stationary phase and gradient elution. Detection is by UV absorbance. The total run time, including column re-equilibration, is 13 min. Single-laboratory validation (SLV) has been performed on the method to determine the repeatability, accuracy, selectivity, LOD, LOQ, ruggedness, and linearity for (+)-catechin, (-)-epicatechin, (-)-epicatechin gallate, (-)-epigallocatechin, (-)-gallocatechin gallate, (-)-epigallocatechin gallate, and (+)-gallocatechin, as well as caffeine. Repeatability precision and recovery results met AOAC guidelines for SLV studies for all catechins and caffeine down to a level of approximately 20 mg/g. Finished products containing high concentrations of minerals require the use of EDTA to prevent decomposition of the catechins.
Subjects:
[+]
Article Caffeine Camellia Sinensis Catechin Chromatography, High Pressure Liquid Reproducibility Of Results
Source:
J AOAC Int. 96(5):933-941
Pubmed ID:
24282928
Pubmed Central ID:
PMC4115333
Document Type:
Journal Article
Funding:
223-01-2464/NoneNone/ ; 223-01-2464/CD/ODCDC CDC HHSUnited States/
223-01-2464/NoneNone/ ; 223-01-2464/CD/ODCDC CDC HHSUnited States/ Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:d884821189f7af8b3a9a0edc9baccbe75822f39a821fc5195ee0537b34b437fd
Download URL:
https://stacks.cdc.gov/view/cdc/110933/cdc_110933_DS1.pdf
File Type:

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