Early Pregnancy Prediction of Spontaneous Preterm Birth Prior to 32 Completed Weeks of Pregnancy Using Plasma RNA

Weiner, Carl P.; Dong, Yafeng; Zhou, Helen; Cuckle, Howard; Ramsey, Risa; Egerman, Robert; Buhimschi, Irina; Buhimschi, Catalin

doi:10.1111/1471-0528.16736

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Early Pregnancy Prediction of Spontaneous Preterm Birth Prior to 32 Completed Weeks of Pregnancy Using Plasma RNA

10 2021
By Weiner, Carl P. ; Dong, Yafeng ; Zhou, Helen ; ...
http://dx.doi.org/10.1111/1471-0528.16736
Source: BJOG. 128(11):1870-1880

[PDF-936.75 KB]

English

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Details:

Alternative Title:

BJOG
Personal Author:

Weiner, Carl P. ; Dong, Yafeng ; Zhou, Helen ; Cuckle, Howard ; Ramsey, Risa ; ... More +

Weiner, Carl P. ; Dong, Yafeng ; Zhou, Helen ; Cuckle, Howard ; Ramsey, Risa ; Egerman, Robert ; Buhimschi, Irina ; Buhimschi, Catalin Less -
Description:

Objective:

Compare the second trimester plasma cell-free (PCF) transcriptome of women who delivered at term to women with spontaneous preterm birth (sPTB)≦32weeks and identify/validate PCF ribonucleic acid (RNA) markers present by 16weeks.

Design:

Prospective case-control study.

Setting:

Academic tertiary care centre.

Population:

Pregnant women with known outcomes prospectively sampled.

Methods:

PCF RNA extracted from women at 22–24weeks (5 sPTB≦32weeks and 5 term) was hybridised to gene expression arrays. Differentially regulated RNAs for sPTB≦32weeks were initially selected based on p-value compared to control (p<0.01) and fold change (≥1.5x). Potential markers were then reordered by narrowness of distribution. Final marker selection was made by searching the Metacore™ database to determine whether the PCF RNA interacted with a reported set of myometrial Preterm Initiator genes. RNAs were confirmed by qRT-PCR and tested in a second group of 40 women (20 with sPTB ≦32weeks (mean gestation 26.5, standard deviation ±2.6weeks), 20 with spontaneous term delivery (40.1±0.9weeks) sampled 16–19+5weeks.

Main outcome measure:

Identification of PCF RNAs predictive of sPTB≦32weeks.

Results:

297 PCR RNAs were differentially expressed in sPTB≦32weeks. Further selection retained 99 RNAs (86 mRNAs and 13 miRs) and 5 of these interacted in silica with 7 Preterm Initiator genes. Four of 5 RNAs were confirmed and tested on the validation group. The expression of each confirmed PCF RNA was significantly higher in sPTB≦32weeks. In vitro study of the 4 mRNAs revealed higher expression in placenta of women with sPTB≦32weeks and the potential to interfere with myometrial quiescence.

Conclusions:

The PCF RNA markers are highly associated with sPTB≦32weeks by 16weeks.
Keywords:

[+]

Adult Biomarkers Case-Control Studies Female Humans Infant, Newborn Maternal Serum Screening Tests MicroRNAs Plasma RNA Precision Medicine Predictive Value Of Tests Pregnancy Pregnancy Trimester, Second Premature Birth Preterm Birth Prospective Studies RNA RNA, Messenger Transcriptome
Source:

BJOG. 128(11):1870-1880
Pubmed ID:

33969600
Pubmed Central ID:

PMC8455415
Document Type:

Journal Article
Funding:

P20 RR016475/RR/NCRR NIH HHSUnited States/ ; UO1/DP000187/CC/CDC HHSUnited States/ ; 2009/Burroughs Wellcome Fund/ ; P20 GM103418/GM/NIGMS NIH HHSUnited States/ ; U01 DP000187/DP/NCCDPHP CDC HHSUnited States/ ; ... More +

P20 RR016475/RR/NCRR NIH HHSUnited States/ ; UO1/DP000187/CC/CDC HHSUnited States/ ; 2009/Burroughs Wellcome Fund/ ; P20 GM103418/GM/NIGMS NIH HHSUnited States/ ; U01 DP000187/DP/NCCDPHP CDC HHSUnited States/ ; WT_/Wellcome TrustUnited Kingdom/ Less -
Volume:

128
Issue:

11
Collection(s):

CDC Public Access
Main Document Checksum:

[+]

urn:sha256:174f105dd060d7ecf7575d56a57c103340c67a10f415c76909658b1114bef9c2
Download URL:

https://stacks.cdc.gov/view/cdc/110301/cdc_110301_DS1.pdf
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