Maternal Food Restriction-induced Intrauterine Growth Restriction in a Rat Model Leads to Sex-specific Adipogenic Programming

Details

• Alternative Title:
  FASEB J
• Personal Author:
  Sreekantha, Sreevidya ; Wang, Ying ; Sakurai, Reiko ; Liu, Jie ; Rehan, Virender K.
• Description:
  Intrauterine growth restriction (IUGR) leads to offspring obesity. In a maternal food restriction (MFR) during pregnancy-related IUGR rat model, bone marrow stem cells showed enhanced adipogenic programming; however, the effect of IUGR on white adipose tissue (WAT) progenitors is unknown. Here, by mRNA and functional profiling, we determined sex-specific adipogenic programming of WAT progenitors isolated from pups on the postnatal day (PND) 1 and 21. On PND1, PPARγ and Pref-1 expression was significantly downregulated in preadipocytes of both MFR males and females; however, at PND21, preadipocytes of MFR males showed upregulation in these genes. Even following adipogenic induction, both male and female MFR adipocytes exhibited lower PPARγ, ADRP, and adiponectin levels at PND1; however, at PND21 MFR male adipocytes showed an upward trend in the expression of these genes. An adipogenesis-specific RT-PCR array showed that male MFR adipocytes were programmed to exhibit stronger adipogenic propensity than females. Last, serum sex hormone and adipocyte estrogen/testosterone receptor expression profiles provide preliminary insights into the possible mechanism underlying sex-specific adipogenic programming in the IUGR offspring. In summary, IUGR programs WAT preadipocytes to greater adipogenic potential in males. Although the altered adipogenic programming following MFR was detectable at PND1, the changes were more pronounced at PND21, suggesting a potential role of postnatal nutrition in facilitating the sex-specific adipogenic programming in the IUGR offspring.
• Subjects:
  Adipocytes Adipogenesis Adiponectin Adipose Tissue, White Animals Animals, Newborn Calcium-Binding Proteins Caloric Restriction Cells, Cultured Down-Regulation Female Fetal Growth Retardation Male Maternal Nutritional Physiological Phenomena Models, Animal Nutritional Status PPAR Gamma Pregnancy Prenatal Exposure Delayed Effects Rats Rats, Sprague-Dawley RNA, Messenger Up-Regulation Uterus

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• Keywords:
  Adipocytes Buprenorphine Methadone Mortality Naltrexone Obesity Preadipocytes Treatment White Adipose Tissue
• Source:
  FASEB J. 34(12):16073-16085
• Pubmed ID:
  33047380
• Pubmed Central ID:
  PMC8121157
• Document Type:
  Journal Article
• Funding:
  R21 HD071731/HD/NICHD NIH HHSUnited States/ ; HD071731/HHS ; NIH ; National Heart, Lung, and Blood Institute (NHLBI)/ ; R01 HL151769/HL/NHLBI NIH HHSUnited States/ ; HD058948/HHS ; NIH ; National Heart, Lung, and Blood Institute (NHLBI)/ ; HL152915/HHS ; NIH ; National Heart, Lung, and Blood Institute (NHLBI)/ ; R41 HL152915/HL/NHLBI NIH HHSUnited States/ ; R03 HD058948/HD/NICHD NIH HHSUnited States/ ; HD127237/HHS ; NIH ; National Heart, Lung, and Blood Institute (NHLBI)/ ; R21 HL107118/HL/NHLBI NIH HHSUnited States/ ; K01 IP000050/IP/NCIRD CDC HHSUnited States/ ; R01 HL127237/HL/NHLBI NIH HHSUnited States/ ; HL107118/HHS ; NIH ; National Heart, Lung, and Blood Institute (NHLBI)/
• Volume:
  34
• Issue:
  12
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:26e82a1bdb0209cef08a644dc43b7f8f5e6a0ec034b3422be6c3fd801afccd9a8f1b1f25a7a985775c0b7c27b6eb89e9df769d81f422ca1886a792377808ad06
• Download URL:
  https://stacks.cdc.gov/view/cdc/106230/cdc_106230_DS1.pdf
• File Type:
  [PDF - 881.72 KB ]