Long-Term Stability of 18 Nutritional Biomarkers Stored at −20 °C and 5 °C for up to 12 Months
Advanced Search
Select up to three search categories and corresponding keywords using the fields to the right. Refer to the Help section for more detailed instructions.

Search our Collections & Repository

All these words:

For very narrow results

This exact word or phrase:

When looking for a specific result

Any of these words:

Best used for discovery & interchangable words

None of these words:

Recommended to be used in conjunction with other fields

Language:

Dates

Publication Date Range:

to

Document Data

Title:

Document Type:

Library

Collection:

Series:

People

Author:

Help
Clear All

Query Builder

Query box

Help
Clear All

For additional assistance using the Custom Query please check out our Help Page

i

Long-Term Stability of 18 Nutritional Biomarkers Stored at −20 °C and 5 °C for up to 12 Months

Filetype[PDF-321.04 KB]


  • English

  • Details:

    • Alternative Title:
      J Appl Lab Med
    • Description:
      Background:

      Consistent information on long-term storage stability for a broad range of nutritional biomarkers is lacking. We investigated the stability of 18 biomarkers stored at suboptimal temperatures (−20 °C and 5 °C) for up to 12 months.

      Methods:

      Multiple vials of serum or whole blood pools (3 concentrations) were stored at −20 °C or 5 °C, removed from the −20 °C freezer after 3, 6, 9, and 12 months and from the 5 °C refrigerator after 6 and 12 months, and placed into a −70 °C freezer until analysis at study completion. Vials stored continuously at −70 °C were used as the reference condition for optimal storage. We measured 18 biomarkers: 4 iron status, 1 inflammation, 8 water-soluble vitamin, and 5 fat-soluble vitamin. For each temperature, we calculated geometric mean concentrations and average percent changes of geometric means across pools relative to the reference condition estimated from a linear mixed model.

      Results:

      Most biomarkers (13 of 18) showed no difference in concentration after 12 months of storage at −20 °C. Serum ferritin (1.5%), soluble transferrin receptor (−1.7%), and folate (−10.5%) showed small to moderate significant changes at 6 months, but changes were acceptable based on biologic variability. Serum pyridoxal-5′-phosphate (−18.6% at 9 months) and vitamin C (−23% at 6 months) showed large and unacceptable changes at −20 °C. All serum fat-soluble vitamins and iron status indicators, vitamin B12, total homocysteine, and methylmalonic acid showed acceptable changes when stored at 5 °C for up to 12 months.

      Conclusions:

      Overall, we found good long-term stability for multiple nutritional biomarkers stored at suboptimal temperatures.

    • Subjects:
    • Pubmed ID:
      33626820
    • Pubmed Central ID:
      PMC7951994
    • Document Type:
    • Collection(s):
    • Main Document Checksum:
    • File Type:

    You May Also Like

    Checkout today's featured content at stacks.cdc.gov