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Multistate Survey of American Dog Ticks (Dermacentor variabilis) for Rickettsia Species
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April 03 2019
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Source: Vector Borne Zoonotic Dis. 19(9):652-657
[PDF-316.35 KB]
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Alternative Title:Vector Borne Zoonotic Dis
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Description:, a common human-biting tick found throughout the eastern half and along the west coast of the United States, is a vector of multiple bacterial pathogens. Historically, | has been considered a primary vector of |, the causative agent of Rocky Mountain spotted fever. A total of 883 adult |, collected between 2012 and 2017 from various locations in 12 states across the United States, were screened for rickettsial DNA. Tick extracts were evaluated using three real-time PCR assays; an |-specific assay, a |-specific assay, and a | genus-specific assay. Sequencing of |A gene amplicons generated using a seminested PCR assay was used to determine the rickettsial species present in positive samples not already identified by species-specific real-time assays. A total of 87 (9.9%) tick extracts contained | DNA and 203 (23%) contained DNA of other rickettsial species, including 47 (5.3%) with |, 11 (1.2%) with |, 2 (0.2%) with |, and 3 (0.3%) with |. Only 1 (0.1%) tick extract contained DNA of |. These data support multiple other contemporary studies that indicate infrequent detection of | in | in North America.
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Pubmed ID:30942664
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Pubmed Central ID:PMC7805563
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Volume:19
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Issue:9
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