One of the challenges of working with highly pathogenic viruses such as EBOV/MARV is that biosafety level 4 (BSL-4) facilities are required to handle the infectious viruses. For the study of GP fusion of many enveloped viruses, this obstacle can be circumvented by a surrogate system called viral pseudotyping.³² This surrogate system has been widely utilized by virologists to study the entry mechanisms of highly pathogenic viruses and to identify and develop antiviral therapeutics, in our³¹ laboratories and other^32–35 laboratories. It is generally accepted that pseudotyped assays for filoviruses and other pathogenic viruses are valid surrogate assays. Thus, many of the antifilovirus compounds displayed in Figure 1 have been identified using a viral pseudotyped assay followed by validation with infectious data. A recent publication from USAMRIID reports a “high degree of correlation” between infectious BSL-4 neutralization assays and pseudotyped BSL-2 fluorescence assays.³³ In this study, we used this system to rapidly identify potent EBOV and MARV entry inhibitors. We have screened a ChemBridge Small Molecule Library of 10,000 compounds for filovirus entry inhibitors using a robust, cell-based high-throughput screening (HTS) protocol.³⁴ Several hit compounds, including a set of the substituted 4-(aminomethyl)benzamides, have been shown to inhibit entry of both EBOV and MARV pseudovirions^35–36. To validate our initial hits, we tested them against wild type EBOV (1976 Mayinga variant) and MARV (Lake Victoria, 2005 Angola) strains in Vero cells.³⁷ One of the 4-(aminomethyl)benzamides, designated as CBS1118 (Figure 2), was found to have EC₅₀ values < 10 μM for both EBOV and MARV, suggesting that this compound has broad-spectrum antifiloviral activity.

The structural modifications on the hit compound CBS1118 were implemented to improve selectivity and potency toward EBOV or MARV through the addition of appropriate substituents in the amide portion of the molecule, the aromatic region or the tertiary amine region as outlined in Figure 2. Upon medicinal chemistry and SAR optimization, we have assembled several sets of modified 4-(aminomethyl)benzamides.

Our general method for the synthesis of 4-(aminomethyl)benzamides is shown in Scheme 1. Treatment of 4-(bromomethyl)benzoic acid esters 1a-e with cyclic amines such as piperidine (2a), morpholine (2b) and 4-methylpiperazine (2c) cleanly produced the 4-(aminomethyl)benzoic acid esters 3a-f. Basic hydrolysis of these esters gave the intermediate benzoic acids, which were subsequently converted to the benzoyl chlorides 4a-f by heating at reflux in thionyl chloride. The benzoyl chlorides 4a-f were coupled with a variety of anilines by utilizing the traditional Schotten-Baumann procedure to provide the 4-(aminomethyl)benzamides 5–35.

The thiophene analogs 38 and 39 were synthesized in a similar manner starting from commercially available methyl 5-(bromomethyl)thiophene-2-carboxylate (36) through the intermediate thiophene-2-carbonyl chloride (37) as shown in Scheme 1.

To create conformationally restricted versions of our scaffold, indolines and other related heterocycles were incorporated into the amide portion of the molecule. These compounds were prepared as shown in Scheme 2. Benzoic acids 4a,c were coupled with substituted indolines 40a-h, again using the Schotten-Baumann procedure, to provide compounds 41–50. Using an analogous procedure, 4-(piperidin-1-ylmethyl)benzoyl chloride (4a) was treated with three different amines 51a-c to provide amides 52–54. The thiophene compound 55, bearing a 5-carboethoxy substituted indoline, was synthesized as a constrained analog of compound 38. Conformational restraints can produce compounds that bind with lower energy barriers by eliminating the entropic preorganization component.

All synthesized compounds were tested against EBOV or MARV GP pseudotyped vesicular stomatitis virus (VSV) in A549 cells and biological data are summarized in Tables 1–3. Toremifene, a small molecule entry inhibitor previously shown to bind and destabilize GP,¹⁸ was used as positive control in this assay. Our SAR studies described here mainly focused on the potency of these compounds for EBOV infection inhibition. It started with the replacement of the piperidine ring in ester 5 (HTS hit CBS1118) with morpholine and N-methylpiperazine, to vary the amine substituent. Both of these modifications decreased potency; compounds 6 and 7 showed only 57% and 29% inhibition at 12.5 μM, respectively. Regioisomer 8, with the methyl ester in the meta-position, was completely inactive. 3-(Aminomethyl)benzamide 9 was much weaker than the original hit compound, 4-(aminomethyl)benzamide 5 (only 13% inhibition vs 74%, respectively). Also, the introduction of a carbonyl linker, such as in compound 10, was unfavorable (12.9% inhibition).

Next, we investigated replacements of the potentially metabolically unstable methyl ester with different bioisosteres. Among the monosubstituted analogs 11–17, only compounds 16 and 17, bearing a lipophilic trifluoromethyl substituent in the para- and meta-positions, were more potent than the hit compound 5 (EC₅₀ = 3.87 and 2.97 μM, respectively, vs 9.86 μM for compound 5), while the replacements with more polar or negatively charged groups provided only less potent inhibitors. Introduction of an additional chlorine group in the aromatic ring was well-tolerated and enhanced the potency of compounds 18 (2.34 μM) and 19 (1.52 μM) and its SCF₃ analog 20 (1.27 μM) over the corresponding monosubstituted compounds 16 and 17 (Table 1).

Among the alkyl substituted analogs, the potency correlated with the size of the alkyl group as follows: 4-cyclopropyl (21, EC₅₀ = 4.57 μM) < 4-isopropyl (22, 2.87 μM) < cyclohexyl (25, 0.99 μM) < 2-cyclopentylacetonitrile (24, 0.73 μM) < di-tert-butyl (23, 0.48 μM). We chose to maintain the bulky cyclohexyl group to explore some further modifications targeting the benzyl ring, such as introduction of fluorine, as in compound 26 (0.38 μM), and introduction of a methyl group in the linker, as in compound 27 (0.33 μM). All three analogs 25-27 showed excellent activity against EBOV pseudovirus and remarkable potency against pseudoviral MARV (Table 1), coupled with improved selectivity. The selectivity index (SI) in Table 1–3 is a ratio between cytotoxicity (CC₅₀) and antiviral activity of the tested compounds. Additional modifications targeted the aniline portion of the scaffold and comprised replacement of the aromatic ring with more flexible linkers, such as alkyl or alkoxy chains between the cyclohexane moiety and the amide. Replacement with a two-carbon linker, as in 28, led to a 2.3-fold drop in potency, while a shorter linker, as in 29, or insertion of an oxygen, as in the ether 30 or acetal 31, caused greater reductions in potency in both pseudoviral assays (Table 2).

Compounds 32 and 33, bearing an adamantyl group in the para-position of the aniline ring and piperidine and morpholine rings attached to the benzylic portion of the molecule, respectively, were both found to possess excellent potency, with EC₅₀ < 100 nM. Since enhancement of potency appeared to be correlated to the lipophilicity of the substituents on the phenyl ring, our objective was to strike a balance between potency and drug-likeness, including a drug-like LogP value. The lipophilicity of our new lead compound 34 was decreased by replacement of the adamantyl group with a 4,4-difluoropiperidinyl group: compound 34 (EC₅₀ value < 1 μM) has a lower cLogP value of 4.71 as compared to the adamantane analog 32 (cLogP = 5.97). The best compound in this series was compound 35, with an EC₅₀ value of 12 nM. This combination of a trifluoromethyl group in the meta-position and 4-methylpiperidinyl in the para-position of the aniline moiety, with morpholine as the cyclic amine, appeared to be the best combination of substituents with respect to potency. Compound 35 displays low cytotoxicity with an excellent SI value.

On the other hand, replacement of the phenyl ring with thiophene was detrimental to activity and compounds 38 and 39 showed only insignificant inhibition at the test concentration. Perhaps this loss of activity with the thiophenes reflects an unfavorable change in the trajectory of the two aromatic substituents on the thiophene ring compared with the phenyl ring.

Table 3 shows the indoline series compounds, which are amide-constrained versions of the benzamide 5. Indoline 41, having a methyl ester at the 5-position, was less potent than the unconstrained version (41.7% vs 74% inhibition, respectively). While introduction of a trifluoromethyl group at the 6-position afforded compound 43 with augmented potency (EC₅₀ = 1.68 μM), its 5-regioisomer 42 showed only minor improvement over the ester 42 (56.4% inhibition at 12.5 μM vs 41.7%, respectively). Among the two halogenated analogs 44 and 45, the 6-chloro substituted compound 44 showed enhanced potency (EC₅₀ = 2.22 μM). The introduction of gem-dimethyl groups in the 3-position of the indoline ring yielded compound 46 with moderate potency; its cyclopropyl analog 47 was less active. The bulky tert-butyl group at the 3-position improved activity, producing analog 50 with an EC₅₀ value of 0.16 μM. Interestingly, compound 48, bearing a methyl group at the benzyl methylene position, was similar in potency to its desmethylated analog 43.

The final iteration of optimization (Table 3) was to replace the anilide aromatic ring with various heterocycles. Pyridine in 52 represents a simple bioisosteric replacement of carbon with nitrogen in compound 16, which led to a drastic decrease in potency (5.4% vs 89.9% inhibition). Tetrahydroquinoline 53 was less potent than its indoline analog 41 and so was indole 54. Replacement of the core phenyl ring with thiophene as in compound 55 had a lesser (negative) impact on the potency than in the aniline series.

The HTS hit, compound 5, surprisingly, was more potent toward Marburg virus than Ebola virus, as shown in Table 1. The variety of structural modifications described herein, especially the incorporation of bulky alkyl or cycloalkyl substituents, gave compounds either almost equipotent against both viruses, or more potent against Ebola virus. It has been our experience that structural changes which enhance activity for Ebola virus generally do the same for Marburg virus, even though the potencies may differ.

All new compounds with promising activity in the pseudovirus assay were evaluated in wild type EBOV and MARV virus assays in HeLa cells. As shown in Table 4, several compounds have greatly improved potency against EBOV, combined with low cytotoxicity. For example, compounds 32 and 35 display EC₅₀ values of 0.11 μM and 0.31 μM, respectively, with excellent SI values. In addition, they both retained good potency against MARV (EC₅₀ = 1.23 μM for 32 and 0.82 μM for 35). These results imply that the newly developed 4-(aminomethyl)benzamides are broad-spectrum antifiloviral agents that target the GP-mediated entry process. In general, there was a good agreement with the EC₅₀ values for a compound between the HIV-based pseudoviral entry assay and the EBOV and live virus assays, as shown in Tables 1–4. However, discrepancies were observed for some compounds, which is not surprising since these are different bioassays and the discrepancies demonstrate the need for validating antiviral activity for the lead compounds with infectious EBOV and MARV. Remarkably, the HTS hit 5 (CBS1118), which displayed an EC₅₀ value of 2.83 μM against the Ebola strain in Vero E6 cells, was found to be inactive when tested in the HeLa cell line. This inconsistency could be attributed to a nonspecific esterase activity in HeLa cells resulting in metabolism of the parent ester to the inactive carboxylic acid 11. In addition to the higher potency and selectivity displayed by our new hit and lead compounds (Table 4), we have attempted to remove metabolic liabilities.

The recently published co-crystal structure of GP with toremifene shows that this ligand binds in a cavity between the attachment (GP1) and fusion (GP2) subunits at the entrance to a large tunnel that links with equivalent tunnels from the other monomers of the trimer at the three-fold axis (Figure 3a,b).¹⁸ The key interactions within the binding site are formed between the aromatic rings of toremifene and Y517 and T519 on GP2, as well as multiple hydrophobic residues on the GP2 internal fusion loop such as L554 and M548. Additional interactions include hydrogen bonding with R64 and the side chain of L186 on the GP1 (Figure 3b).

To investigate the potential binding mode of our new compounds, selected 4-(aminomethyl)bezamides were docked into the toremifene hydrophobic binding site of EBOV GP (PDB 5JQ7) as exemplified by 49 and 32 (Figure 3c,d). Docking of indoline 49 (Figure 3c) shows that flexibility of this ligand allows the range of amino acid interactions observed for toremifene, including π-stacking of the indoline heterocycle with Y517 (Figure 3b). While ligand 32 does not appear to be within an acceptable proximity to hydrogen bond with Y517, the carbonyl group of 32 is able to hydrogen bond with T519. The adamantyl ring of 32 is involved in multiple interactions with L554, M548 and other residues in this hydrophobic pocket. Since the adamantyl moiety makes compound 32 more rigid, its binding mode is different from compounds where the amide portion is smaller. Although this ligand is more shifted toward the side chain of R64, it is well-accommodated in the pocket and it overlaps with toremifene. Thus, the modeling results suggest that our 4-(aminomethyl)benzamides can bind to GP in the toremifene site. A recent report on the structural studies of the binding mode of another set of anti-EBOV small molecule agents supports this statement.³⁸

To provide support for the conclusions of the docking studies, including the binding of 32 and 49 to the toremifene binding site, two GP mutants were used: Y517S and T519V.

In the Y517S mutant, concentration-response was right-shifted for toremifene and to a lesser extent 49 (Figure 4a), but not shifted for 32 (Figure 4b). In the T519V mutant, response was right-shifted for toremifene and, to a lesser extent, for 32 (Figure 4b). These results are in agreement with the docking studies and provide further support for the proposed MOA that our new compounds inhibit viral entry by binding directly to the EBOV-GP in a similar fashion to toremifene.

Compound metabolic stability and CYP inhibition data are shown in Table 5 for selected compounds. Indolines 43 and 50 were found to be surprisingly unstable in rat plasma, and both rat and human liver microsomes, while compounds 20, 23, 32, 33 and 35 possessed acceptable stability and were tested for inhibition of the two most important Phase 1 metabolism human CYPs, CYP3A4 and CYP2C9. Although CYP inhibition is observed at concentrations higher than those required for antiviral activity, the observed weak inhibition of CYP2C9 will need to be considered in further lead optimization studies with this group of potential drug candidates.

EC₅₀ values obtained from HIV/EBOV-GP or HIV/MARV-GP pseudotype and EBOV (1976 Mayinga variant) or MARV (Lake Victoria, 2005 Angola) assays are reported as the average of two or more replicates. All solvents and reagents were purchased from commercial suppliers and used without further purification.

¹H and ¹³C NMR spectra were recorded on Bruker DPX-400 or AVANCE-400 spectrometers, at 400 MHz and 100 MHz, respectively. NMR chemical shifts were reported in δ (ppm) using residual solvent peaks as standards (CDCl₃: 7.26 ppm (¹H), 77.23 ppm (¹³C); CD₃OD: 3.31 ppm (¹H), 49.15 ppm (¹³C); DMSO-d₆: 2.50 ppm (¹H), 39.52 ppm (¹³C)). Mass spectra were measured in the ESI mode at an ionization potential of 70 eV with an LC-MSD on Hewlett-Packard spectrometer. All key compounds (5–35, 38, 39, 41–50, 52–55) possess a purity of at least 95% as assessed by analytical reversed phase HPLC using an ACE 3AQ C₈ column (150 × 4.6 mm, particle size 3 μM) with detection at 254 and 280 nm on a Shimadzu SPD-20A VP detector; flow rate =1.0 mL/min; gradient of 10–95% acetonitrile in water (both containing 0.1 vol % of FA) in 20 min.

Low-passage A549 cells were infected by a HIV/MARV-GP or HIV/EBOV-GP pseudotype virus containing a luciferase reporter gene in the presence and absence of compounds at 12.5 μM.⁴⁵ Plates were incubated for 48 h and the infection was then quantified by the luciferase activity of the infected A549 cells using the Neolite Reporter Gene Assay System (Perkin Elmer; Boston, MA). Compounds that showed ≥70% inhibition at 12.5 μM concentration of EBOV or MARV pseudovirions^{35, 46} were further evaluated to determine their EC₅₀ values using four-parameter logistic regression analysis in GraphPad (Graphpad Software; San Diego, CA).

Wild type Ebola virus or Marburg virus was used for testing the efficacy of compounds. All viral infections were done in a BSL-4 facility at the Texas Biomedical Research Institute and in the NEIDL, Boston University. Briefly, 4,000 HeLa cells per well were grown overnight in 384-well tissue culture plates; the volume of DMEM (Fisher scientific, Cat#MT10017CV) culture media supplemented with 10% fetal bovine serum (Gemini Bio-Products, Cat#100106) was 25 μL. On the day of assay, test drugs were diluted to 1 mM concentration in complete media. A 25 μL volume of this mixture was added to the cells already containing 25 μL of media to achieve a concentration of 500 μM. All treatments were done in triplicate. A 25 μL volume of media was removed from the first wells and added to the next well. This type of serial dilution was performed 12 times and treated cells were then incubated at 37 °C in a humidified CO₂ incubator for 1 hour. Final concentrations of 250, 125, 62.5, 31.25, 15.62, 7.81, 3.9, 1.9, 0.97, 0.48, 0.24 and 0.12 μM were achieved upon addition of 25 μL of infection mix containing EBOV or MARV virus. Bafilomycin, at a final concentration of 10 nM, was used as a positive control drug. Infections were designed to achieve a MOI of 0.05 to 0.15. Infected cells were incubated for 24 hours. At 24 hours post-infection, cells were fixed by immersing the plates in formalin for 24 hours at 4 °C. Fixed plates were decontaminated and removed from the BSL-4 facility. Formalin from fixed plates was decanted and plates were washed with PBS. EBOV (or MARV)-infected cells were stained for nuclei using the Hoechst stain at 1:50,000 dilution and virus specific antibody (IBT Bioservices) followed by a fluorescently labeled secondary antibody (Alexa488). The plates were imaged. Nuclei (blue) and infected cells (green) were counted using CellProfiler software (Broad Institute) Version 2.1.1. Total number of nuclei (blue) was used as a proxy for cell numbers and a loss of cell number was assumed to reflect cytotoxicity. Concentrations where total cell numbers were 20% less than the control were rejected from the analysis.

To a solution of methyl bromide (1 eq) in anhydrous dichloromethane (3 mL/mmol), cyclic amine (1.0 eq) and triethylamine (5 eq) were added and the reaction mixture was stirred at room temperature under argon for 8–24 hours to reaction completion as monitored by TLC. The solvents were evaporated, and the solid was treated with a mixture of dichloromethane and sodium bicarbonate (aq). The organic phase was collected, washed with water and brine, and dried over sodium sulfate. Solvent was removed under vacuum to provide crude product, which was further purified by flash chromatography (CombiFlash) by elution with methanol in dichloromethane (5–30%) to yield the desired product.

A solution of methyl ester in tetrahydrofuran (3 mL/mmol), methanol (1 mL/mmol) and water (0.5 mL/mmol) was treated with sodium hydroxide (2 eq). The reaction mixture was stirred overnight at room temperature. The solvents were removed under vacuum and the residue was treated with thionyl chloride (2 mL/mmol). The reaction mixture was heated at reflux for 1 h and concentrated under vacuum. The product was used in next step without purification.

To a solution of acyl chloride (1 eq) in anhydrous dichloromethane (3 mL/mmol), was added amine (0.8 eq), followed by triethylamine (5 eq), and the reaction mixture was stirred at room temperature under argon for 8–24 hours until reaction was complete as monitored by TLC. The reaction mixture was diluted with dichloromethane and washed with sodium bicarbonate (aq), and brine, and dried over sodium sulfate. Solvent was removed under vacuum to provide crude product, which was further purified by flash chromatography (CombiFlash) by elution with methanol in dichloromethane (5–30%) to yield the desired product.

Yield: 165 mg, 59%. HPLC purity: 99%. ¹H NMR (400 MHz, DMSO-d₆) δ 1.39 (m, 2H), 1.49 (m, 4H), 2.33 (m, 4H), 3.50 (s, 2H), 3.84 (s, 3H), 7.44 (d, J = 8.1 Hz, 2H), 7.90 (d, J = 8.1 Hz, 2H), 7.95 (m, 4H), 10.51 (s, 1H). ¹³C NMR (100 MHz, DMSO-d₆) δ 24.4, 26.0, 52.3, 54.3, 62.8, 119.9, 124.6, 128.1, 129.0, 130.5, 133.5, 143.5, 144.1, 166.2, 166.3. HRMS (ESI) calculated for C₂₁H₂₅N₂O₃ ([M+H]⁺): 353.1865, found: 353.1860.

Yield: 150 mg, 66%. HPLC purity: 99%. ¹H NMR (400 MHz, CDCl₃) δ 2.50–2.51 (m, 4H), 3.60 (s, 2H), 3.73 (t, J = 4.5 Hz, 4H), 7.47 (d, J = 8.1 Hz, 2H), 7.83 (d, J = 8.8 Hz, 2H), 7.92 (d, J = 8.1 Hz, 2H), 8.01 (d, J = 8.8 Hz, 2H). ¹³C NMR (100 MHz, CDCl₃) δ 55.5, 57.2, 66.6, 70.5, 123.7, 129.1, 131.5, 133.2, 134.2, 137.5, 145.4, 147.0, 171.0, 171.2. HRMS (ESI) calculated for C₂₀H₂₃N₂O₄ ([M+H]⁺): 355.1658, found: 355.1651.

Yield: 140 mg, 38%. HPLC purity: 99%. ¹H NMR (400 MHz, MeOD) δ 2.31 (s, 3H), 2.47–2.54 (m, 8H), 3.63 (s, 2H), 3.91 (s, 3H), 7.50 (d, J = 8.2 Hz, 2H), 7.87 (dd, J = 1.9, 7.1 Hz, 2H), 7.91 (d, J = 8.2 Hz, 2H), 8.02 (dd, J = 1.9, 7.1 Hz, 2H). ¹³C NMR (100 MHz, MeOD) δ 44.4, 51.0, 52.0, 54.2, 61.7, 119.6, 125.2, 127.3, 129.1, 130.0, 133.5, 141.9, 143.2, 166.7, 167.2. HRMS (ESI) calculated for C₂₁H₂₆N₃O₃ ([M+H]⁺): 368.1974, found: 368.1977.

Yield: 115 mg, 83%. HPLC purity: 99%. ¹H NMR (400 MHz, CDCl₃) δ 1.46–1.47 (m, 2H), 1.57–1.63 (m, 4H), 2.40 (m, 4H), 3.54 (s, 2H), 3.93 (s, 3H), 7.45–7.49 (m, 3H), 7.83–7.85 (m, 3H), 7.95 (s, 1H), 8.07 (dd, J = 1.1, 8.1 Hz, 1H), 8.15 (s, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 24.2, 25.8, 52.2, 54.5, 63.2, 121.0, 124.7, 125.4, 127.0, 129.1, 129.3, 130.8, 133.1, 128.3, 143.2, 165.8, 166.7. HRMS (ESI) calculated for C₂₁H₂₅N₂O₃ ([M+H]⁺): 353.1865, found: 353.1865.

Yield: 122 mg, 54%. HPLC purity: 99%. ¹H NMR (400 MHz, CDCl₃) δ 1.46 (m, 2H), 1.59 (m, 4H), 2.40 (m, 4H), 3.52 (d, J = 5.4 Hz, 2H), 3.92 (s, 3H), 7.40–7.46 (m, 1H), 7.50–7.51 (m, 1H), 7.72 (d, J = 8.7 Hz, 3H), 7.86 (s, 1H), 8.03–8.07 (m, 2H), 8.20–8.30 (m, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 24.2, 25.8, 52.0, 54.5, 63.3, 119.16, 119.19, 125.6, 125.9, 127.5, 128.6, 130.8, 132.9, 134.4, 139.5, 142.2, 142.3, 165.9, 166.6. HRMS (ESI) calculated for C₂₁H₂₅N₂O₃ ([M+H]⁺): 353.1865, found: 353.1863.

Yield: 88 mg, 22%. HPLC purity: 97%. ¹H NMR (400 MHz, CDCl₃) δ 1.49 (m, 2H), 1.69 (m, 4H), 3.26 (m, 2H), 3.74 (m, 2H), 3.92 (s, 3H), 7.20 (d, J = 8.2 Hz, 2H), 7.79 (d, J = 8.2 Hz, 2H), 7.93 (d, J = 8.8 Hz, 2H), 8.04 (d, J = 8.8 Hz, 2H), 9.52 (s, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 24.3, 24.4, 25.5, 26.4, 43.2, 48.7, 51.9, 119.4, 125.4, 126.4, 127.8, 130.6, 135.7, 138.8, 142.9, 165.7, 166.7, 169.5. HRMS (ESI) calculated for C₂₁H₂₃N₂O₄ ([M+H]⁺): 367.1658, found: 367.1650.

Yield: 8.0 mg, 27%. HPLC purity: 98%. ¹H NMR (400 MHz, MeOD) δ 1.47–1.48 (m, 2H), 1.59–1.61 (m, 4H), 2.44 (m, 4H), 3.53 (s, 1H), 3.56 (s, 1H), 7.45 (d, J = 8.2 Hz, 2H), 7.66 (d, J = 9.7 Hz, 2H), 7.92–7.97 (m, 4H). ¹³C NMR (100 MHz, MeOD) δ 23.7, 25.1, 54.0, 62.8, 120.0, 127.3, 129.2, 129.5, 132.9, 134.6, 141.0, 142.5, 167.5, 173.9. HRMS (ESI) calculated for C₂₀H₂₃N₂O₃ ([M+H]⁺): 339.1709, found: 339.1712.

Yield: 76 mg, 56%. HPLC purity: 98%. ¹H NMR (400 MHz, DMSO-d₆) δ 1.39 (m, 2H), 1.49 (m, 4H), 2.33 (m, 4H), 3.18 (s, 3H), 3.31 (s, 2H), 7.45 (d, J = 8.1 Hz, 2H), 7.91 (m, 4H), 8.03 (d, J = 8.7 Hz, 2H), 10.60 (s, 1H). ¹³C NMR (100 MHz, DMSO-d₆) δ 24.3, 26.0, 44.2, 54.3, 62.8, 120.3, 128.2, 128.4, 129.0, 133.3, 135.4, 143.6, 144.2, 166.4. HRMS (ESI) calculated for C₂₀H₂₅N₂O₃S ([M+H]⁺): 373.1586, found: 373.1582.

Yield: 70 mg, 46%. HPLC purity: 99%. ¹H NMR (400 MHz, CDCl₃) δ 1.42 (m, 2H), 1.55–1.57 (m, 4H), 2.39 (m, 4H), 2.55 (s, 3H), 3.51 (s, 2H), 7.38 (d, J = 8.1 Hz, 2H), 7.59 (d, J = 8.8 Hz, 2H), 7.82–7.85 (m, 4H). ¹³C NMR (100 MHz, CDCl₃) δ 23.8, 25.2. 28.7, 54.3, 63.2, 120.2, 127.4, 128.1, 129.7, 133.3, 133.4, 142.5, 166.9. HRMS (ESI) calculated for C₂₀H₂₆N₃O₃S ([M+H]⁺): 388.1695, found: 388.1692.

Yield: 79 mg, 55%. HPLC purity: 99%. ¹H NMR (400 MHz, MeOD) δ 1.43–1.45 (m, 2H), 1.55–1.60 (m, 4H), 2.40 (m, 4H), 2.93 (s, 3H), 3.53 (s, 3H), 7.20–7.24 (m, 2H), 7.39–7.41 (m, 2H), 7.64 (dd, J = 2.0, 7.9 Hz, 2H), 7.84 (d, J = 8.2 Hz, 2H). ¹³C NMR (100 MHz, MeOD) δ 23.8, 25.2, 38.4, 54.2, 63.2, 121.7, 121.9, 127.3, 129.7, 133.64, 133.67, 135.5, 166.8. HRMS (ESI) calculated for C₂₀H₂₆N₃O₃S ([M+H]⁺): 388.1695, found: 388.1690.

Yield: 110 mg, 54%. HPLC purity: 97.8%. ¹H NMR (400 MHz, CDCl₃) δ 1.48 (m, 2H), 1.64 (m, 4H), 2.46 (m, 4H), 3.60 (s, 2H), 7.49 (d, J = 8.1 Hz, 2H), 7.65 (d, J = 8.6 Hz, 2H), 7.87 (m, 4H), 8.31 (bs, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 23.9, 25.4, 45.8, 54.4, 62.9, 107.2, 118.8, 119.9, 127.2, 129.7, 133.0, 133.2, 142.1, 165.8. HRMS (ESI) calculated for C₂₀H₂₂N₃O ([M+H]⁺): 320.1763, found: 320.1761.

Yield: 91 mg, 68%. HPLC purity: 99%. ¹H NMR (400 MHz, MeOD) δ 1.49 (m, 2H), 1.61–1.64 (m, 4H), 2.47 (m, 4H), 3.60 (s, 2H), 7.49 (d, J = 8.0 Hz, 2H), 7.63 (d, J = 8.3 Hz, 2H), 7.92–7.94 (m, 4H). ¹³C NMR (100 MHz, MeOD) δ 27.7, 29.1, 67.0, 124.2, 129.71, 129.75, 129.9, 131.4, 133.7, 137.4, 145.1, 145.7, 171.1. HRMS (ESI) calculated for C₂₀H₂₂F₃N₂O ([M+H]⁺): 363.1684, found: 363.1688.

Yield: 48 mg, 72%. HPLC purity: 99%. ¹H NMR (400 MHz, CDCl₃) δ 1.40–1.47 (m, 2H), 1.57–1.62 (m, 4H), 2.39 (m, 4H), 3.52 (s, 2H), 7.38–7.47 (m, 4H), 7.80 (d, J = 8.1 Hz, 2H), 7.87 (d, J = 8.1 Hz, 1H), 7.97 (s, 1H), 8.36 (s, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 24.2, 25.8, 54.5, 63.2, 117.0, 117.04, 120.9, 1225, 123.3, 125.2, 127.0, 129.45, 129.55, 131.2, 131.5, 132.9, 138.6, 143.2, 166.0. HRMS (ESI) calculated for C₂₀H₂₂F₃N₂O ([M+H]⁺): 363.1684, found: 363.1680.

Yield: 49 mg, 58%. HPLC purity: 98.8%. ¹H NMR (400 MHz, CDCl₃) δ 1.40–1.44 (m, 2H), 1.51–1.55 (m, 4H), 2.36 (m, 4H), 3.47 (s, 2H), 7.35 (d, J = 7.8 Hz, 2H), 7.56 (d, J = 8.6 Hz, 1H), 7.66 (d, J = 8.6 Hz, 1H), 7.79 (d, J = 7.8 Hz, 2H), 7.95 (s, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 23.8, 25.2, 54.3, 63.2, 117.6, 121.5, 122.3, 123.0, 123.4, 124.2, 127.4, 127.9, 127.95, 129.7, 132.6, 133.1, 141.7, 142.6, 166.8. HRMS (ESI) calculated for C₂₀H₂₁ClF₃N₂O ([M+H]⁺): 397.1295, found: 397.1293.

Yield: 52 mg, 62%. HPLC purity: 99%. ¹H NMR (400 MHz, CDCl₃) δ 1.46–1.49 (m, 2H), 1.58–1.63 (m, 4H), 2.41 (m, 4H), 3.51 (s, 2H), 7.45 (d, J = 8.1 Hz, 2H), 7.49 (d, J = 8.7 Hz, 1H), 7.81 (d, J = 8.1 Hz, 2H), 7.89 (dd, J = 2.7, 8.7 Hz, 2H), 7.99 (d, J = 2.7 Hz, 1H), 8.11–8.20 (m, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 24.1, 25.8, 54.5, 63.1, 119.3, 119.35, 121.2, 123.9, 124.3, 126.9, 127.1, 128.5, 128.8, 129.4, 131.9, 132.6, 136.9, 143.4, 166.1. HRMS (ESI) calculated for C₂₀H₂₁ClF₃N₂O ([M+H]⁺): 397.1295, found: 397.1299.

Yield: 45 mg, 25%. HPLC purity: 99.9%. ¹H NMR (400 MHz, CDCl₃) δ 1.40–1.48 (m, 2H), 1.58–1.63 (m, 4H), 2.40 (m, 4H), 3.55 (s, 2H), 7.49 (d, J = 8.1 Hz, 2H), 7.60 (dd, J = 2.0, 8.5 Hz, 1H), 7.75 (d, J = 8.5 Hz, 1H),7.81 (d, J = 8.1 Hz, 2H),. 7.90 (b.s, 1H), 8.03 (d, J = 2.0 Hz, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 24.2, 26.0, 54.6, 63.3, 118.1, 118.7, 121.4, 127.0, 129.4, 132.4, 139.4, 141.2, 141.8, 144.2, 166.1. HRMS (ESI) calculated for C₂₀H₂₁ClF₃N₂OS ([M+H]⁺): 429.1015, found: 429.1017.

Yield: 34 mg, 45%. HPLC purity: 99.7%. ¹H NMR (400 MHz, CDCl₃) δ 0.61–0.65 (m, 2H), 0.90–0.94 (m, 2H), 1.42–1.43 (m, 2H), 1.53–1.59 (m, 4H), 1.82–1.89 (m, 1H), 2.37 (m, 4H), 3.49 (s, 2H), 7.01 (d, J = 6.5 Hz, 2H), 7.36 (d, J = 8.3 Hz, 2H), 7.50 (d, J = 8.3 Hz, 2H), 7.78 (d, J = 6.5 Hz, 2H). ¹³C NMR (100 MHz, CDCl₃) δ 8.9, 14.9, 24.0, 25.4, 54.4, 63.3, 120.5, 120.6, 126.1, 127.1, 129.6, 133.8, 135.5, 140.2, 141.5, 166.1. HRMS (ESI) calculated for C₂₂H₂₇N₂O ([M+H]⁺): 335.2123, found: 335.2126.

Yield: 46 mg, 32%. HPLC purity: 97.9%. ¹H NMR (400 MHz, CDCl₃) δ 1.27 (d, J = 6.8 Hz, 6H), 1.49 (m, 2H), 1.59 (m, 4H), 2.40 (m, 4H), 2.93 (quintet, J = 6.8 Hz, 1H), 3.54 (s, 2H), 7.23 (d, J = 8.2 Hz, 2H), 7.43 (d, J = 7.8 Hz, 2H), 7.57 (d, J = 8.2 Hz, 1H), 7.82 (d, J = 7.8 Hz, 2H), 7.95 (b.s, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 24.0, 24.3, 26.0, 33.6, 54.5, 63.4, 120.4, 126.9, 129.3, 133.6, 135.7, 143.1, 145.2, 165.6. HRMS (ESI) calculated for C₂₂H₂₉N₂O ([M+H]⁺): 337.2280, found: 337.2280.

Yield: 24 mg, 28%. HPLC purity: 97.7%. ¹H NMR (400 MHz, MeOD) δ 1.36 (s, 9H), 1.44 (s, 9H), 1.51 (m, 2H), 1.64 (m, 4H), 2.49 (m, 4H), 3.62 (s, 2H), 7.10 (d, J = 8.2 Hz, 1H), 7.32 (dd, J = 2.2, 8.2, Hz, 1H), 7.51 (d, J = 8.2, Hz, 1H), 7.56 (d, J = 2.2 Hz, 1H), 7.95 (d, J = 7.9 Hz, 2H). ¹³C NMR (100 MHz, MeOD) δ 23.6, 25.1, 30.1, 10.4, 34.2, 34.9, 54.0, 62.7, 123. 4, 123.7, 127.2, 129.6, 130.8, 132.6, 133.5, 141.2, 146.3, 150.1, 168.3. HRMS (ESI) calculated for C₂₇H₃₉N₂O ([M+H]⁺): 407.3062, found: 407.3066.

Yield: 22 mg, 26%. HPLC purity: 98%. ¹H NMR (400 MHz, CDCl₃) δ 1.46–1.47 (m, 2H), 2.59–1.64 (m, 4H), 1.94–2.10 (m, 6H), 2.42–2.51 (m, 6H), 3.56 (s, 2H), 7.43 (d, J = 8.6 Hz, 2H), 7.47 (d, J = 8.1 Hz, 2H), 7.70 (d, J = 8.6 Hz, 2H), 7.84 (d, J = 8.1 Hz, 2H), 8.09 (s, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 24.1, 25.7, 40.4, 47.3, 54.5, 63.2, 120.5, 124.4, 126.7, 127.0, 129.5, 133.3, 135.6, 137.6, 142.8, 165.7. HRMS (ESI) calculated for C₂₆H₃₂N₃O ([M+H]⁺): 402.2545, found: 402.2551.

Yield: 14 mg, 17%. HPLC purity: 98%. ¹H NMR (400 MHz, CDCl₃) δ 1.20–1.40 (m, 7H), 1.55 (m, 4H), 1.68–1.80 (m, 5H), 2.37–2.43 (m, 4H), 3.50 (m, 3H), 7.14 (d, J = 6.5 Hz, 2H), 7.36 (d, J = 6.3 Hz, 2H), 7.50 (d, J = 6.5 Hz, 2H), 7.78 (d, J = 6.3 Hz, 2H). ¹³C NMR (100 MHz, CDCl₃) δ 23.8, 25.2, 26.0, 26.7, 34.4, 43.9, 54.3, 63.2, 120.6, 127.1, 129.7, 133.9, 135.7, 144.4, 166.3. HRMS (ESI) calculated for C₂₅H₃₃N₂O ([M+H]⁺): 377.2593, found: 377.2589.

Yield: 43 mg, 55%. HPLC purity: 99.6%. ¹H NMR (400 MHz, MeOD) δ 1.20–1.44 (m, 7H), 1.51–1.62 (m, 4H), 1.71 (d, J = 7.3 Hz, 1H), 1.83 (m, 4H), 2.47 (m, 5H), 3.62 (s, 2H), 7.17 (d, J = 8.4 Hz, 2H), 7.45 (dd, J = 7.5, 10.4 Hz, 1H), 7.54 (d, J = 8.4 Hz, 2H), 7.61 (d, J = 10.4 Hz, 1H), 7.66 (d, J = 7.5 Hz, 1H). ¹³C NMR (100 MHz, MeOD) δ 23.7, 25.2, 26.0, 26.7, 34.4, 44.0, 53.9, 55.3, 114.4, 114.6, 121.0, 122.7, 127.0, 127.2, 132.1, 135.6, 136.2, 136.3, 144.7, 160.0, 162.4, 165.4. HRMS (ESI) calculated for C₂₅H₃₂FN₂O ([M+H]⁺): 395.2499, found: 395.2501.

Yield: 22 mg, 28%. HPLC purity: 98.6%. ¹H NMR (400 MHz, MeOD) δ 1.25–1.35 (m, 11H), 1.38–1.40 (m, 4H), 1.72 (d, J = 6.9 Hz, 1H), 1.84 (m, 4H), 2.41–2.48 (m, 5H), 3.53 (q, J = 6.5 Hz, 1H), 7.18 (d, J = 8.4 Hz, 2H), 7.40 (d, J = 8.2 Hz, 2H), 7.55 (d, J = 8.4 Hz, 2H), 7.87 (d, J = 8.2 Hz, 2H). ¹³C NMR (100 MHz, MeOD) δ 18.5, 23.8, 25.2, 26.0, 26.7, 34.4, 44.0, 51.5, 65.3, 121.1, 126.9, 127.4, 128.1, 133.9, 135.8, 144.5, 166.9. HRMS (ESI) calculated for C₂₆H₃₅N₂O ([M+H]⁺): 391.2749, found: 391.2749.

Yield: 22 mg, 31%. HPLC purity: 99%. ¹H NMR (400 MHz, MeOD) δ 0.91–0.99 (m, 2H), 1.14–1.20 (m, 4H), 1.23–1.26 (m, 1H), 1.32–1.77 (m, 12H), 2.40 (m, 4H), 3.48 (q, J = 6.2 Hz, 2H), 3.50 (s, 2H), 6.11 (m, 1H), 7.38 (d, J = 8.1 Hz, 2H), 7.70 (d, J = 8.1 Hz, 1H). ¹³C NMR (100 MHz, MeOD) δ 24.2, 25.7, 26.2, 26.5, 33.2, 35.4, 37.1, 37.9, 54.4, 63.2, 126.7, 129.2, 133.6, 141.8, 167.3. HRMS (ESI) calculated for C₂₁H₃₂N₂O ([M+H]⁺): 329.2515, found: 329.2591.

Yield: 19 mg, 25%. HPLC purity: 95.3%. ¹H NMR (400 MHz, MeOD) δ 1.34–1.37 (m, 2H), 1.44–1.46 (m, 2H), 1.57–2.03 (m, 9H), 1.86–2.08 (m, 2H), 2.44 (m, 4H), 3.27 (d, J = 6.9 Hz, 2H), 3.55 (s, 2H), 7.37 (d, J = 8.2 Hz, 2H), 7.74 (d, J = 8.2 Hz, 2H). ¹³C NMR (100 MHz, CDCl₃) δ 23.7, 25.1, 26.6, 26.7, 32.7, 33.0, 33.2, 35.9, 44.6, 54.1, 63.0, 127.0, 129.8, 133.4, 140.2, 168.5. HRMS (ESI) calculated for C₂₀H₂₉F₂N₂O ([M+H]⁺): 351.2248, found: 351.2244.

Yield: 21 mg, 29%. HPLC purity: 96.6%. ¹H NMR (400 MHz, CDCl₃) δ 1.28–1.34 (m, 5H), 1.39–1.41 (m, 5H), 1.54–1.60 (m, 5H), 1.74–1.75 (m, 2H), 1.91–1.94 (m, 2H), 2.37–2.45 (m, 4H), 3.26–3.31 (m, 1H), 3.47–3.50 (m, 1H), 3.63 (d, J = 2.3 Hz, 4H), 6.62 (bs, 1H), 7.40 (d, J = 8.1 Hz, 2H), 7.72 (d, J = 8.1 Hz, 2H). ¹³C NMR (100 MHz, CDCl₃) δ 19.0, 24.0, 24.3, 25.7, 25.9, 32.2, 40.0, 51.5, 64.9, 66.4, 77.8, 126.8, 128.0, 133.3, 147.0, 167.3. HRMS (ESI) calculated for C₂₂H₃₅N₂O₂ ([M+H]⁺): 359.2699, found: 359.2695.

Yield: 40 mg, 52%. HPLC purity: 95%. ¹H NMR (400 MHz, CDCl₃) δ 1.37–1.41 (m,8H), 1.56–1.66 (m, 14H), 2.34–2.42 (m, 4H), 3.46–3.57 (m, 2H), 3.68–4.10 (m, 2H), 4.33 (t, J = 3.6 Hz, 1H), 4.34–4.35 (m, 1H), 6.56 (m, 1H), 7.39 (d, J = 8.1 Hz, 2H), 7.73 (d, J = 8.1 Hz, 2H). ¹³C NMR (100 MHz, CDCl₃) δ 19.1, 23.7, 24.0, 24.4, 25.1, 26.0, 34.6, 36.5, 41.9, 51.5, 64.9, 66.3, 74.2, 109.9, 126.8, 127.9, 132.8, 167.5. HRMS (ESI) calculated for C₂₃H₃₅N₂O₃ ([M+H]⁺): 387.2648, found: 387.2649.

Yield: 33 mg, 35%. HPLC purity: 99%. ¹H NMR (400 MHz, CDCl₃) δ 1.46 (m, 2H), 1.61 (m, 4H), 1.79–1.83 (m, 6H), 1.92 (m, 6H), 2.11 (m, 3H), 2.41 (m, 4H), 3.55 (s, 2H), 7.35 (d, J = 8.2 Hz, 2H), 7.43 (d, J = 7.6 Hz, 2H), 7.60 (d, J = 8.2 Hz, 2H), 7.81 (d, J = 7.6 Hz, 2H), 8.02 (s, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 24.2, 25.8, 28.9, 35.9, 36.7, 43.2, 54.4, 63.2, 120.1, 125.4, 127.0, 129.4, 133.8, 135.4, 142.4, 147.7, 165.6. HRMS (ESI) calculated for C₂₉H₃₇N₂O ([M+H]⁺): 429.2906, found: 429.2908.

General Yield: 30 mg, 32%. HPLC purity: 99%. ¹H NMR (400 MHz, CDCl₃) δ 1.79 (m, 26H), 1.92 (m, 6H), 2.12 (m, 3H), 2.47 (m, 4H), 3.57 (s, 2H), 3.73 (m, 4H), 7.35 (d, J = 7.6 Hz, 2H), 7.44 (d, J = 7.2 Hz, 2H), 7.59 (d, J = 7.6 Hz, 2H), 7.82 (d, J = 7.2 Hz, 2H), 7.99 (s, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 28.9, 35.9, 36.7, 43.2, 53.5, 62.8, 66.8, 120.1, 125.4, 127.1, 129.4, 134.1, 135.4, 141.7, 147.8, 165.5. HRMS (ESI) calculated for C₂₈H₃₅N₂O₂ ([M+H]⁺): 431.2699, found: 431.2702.

Yield: 18 mg, 21%. HPLC purity: 97%. ¹H NMR (400 MHz, CDCl₃) δ 1.48–1.65 (m,6H), 2.09–2.16 (m, 4H), 2.47 (m, 4H), 3.34 (m, 4H), 3.61 (s, 2H), 6.95 (d, J = 8.1 Hz, 2H), 7.47 (d, J = 7.3 Hz, 2H), 7.57 (d, J = 8.1 Hz, 2H), 7.82 (d, J = 7.3 Hz, 2H), 7.93 (s, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 24.0, 25.5, 33.3, 33.6, 33.8, 47.1, 54.3, 62.9, 117.4, 121.7, 127.0, 129.6, 130.9, 134.0, 147.2, 165.4. HRMS (ESI) calculated for C₂₄H₃₀F₂N₃O ([M+H]⁺): 414.2357, found: 414.2354.

Yield: 21 mg, 28%. HPLC purity: 98.9%. ¹H NMR (400 MHz, CDCl₃) δ 0.99 (d, J = 5.9 Hz, 3H), 1.36–1.45 (m, 3H), 1.67–1.70 (m, 2H), 2.47 (m, 4H), 2.68 (t, J = 10.8 Hz, 2H), 2.98 (d, J = 10.8 Hz, 2H), 3.57 (s, 2H), 3.73 (m, 4H), 7.32 (d, J = 8.7 Hz, 1H), 7.44 (d, J = 7.6 Hz, 2H), 7.76 (s, 1H), 7.82 (d, J = 7.6 Hz, 2H), 7.89 (d, J = 8.7 Hz, 1H), 8.20 (s, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 22.0, 30.6, 34.7, 53.5, 54.4, 62.8, 66.8, 119.1, 122.3, 124.6, 125.0, 127.1, 127.3, 127.6, 129.4, 133.4, 134.2, 142.1, 150.0, 165.7. HRMS (ESI) calculated for C₂₅H₃₁F₃N₃O₂ ([M+H]⁺): 462.2368, found: 462.2366.

Yield: 71 mg, 44%. HPLC purity: 96%. ¹H NMR (400 MHz, CDCl₃) δ 1.25–1.27 (m, 2H), 1.60–1.61 (m, 4H), 2.46 (m, 4H), 3.70 (s, 2H), 3.92 (s, 3H), 6.93 (m, 1H), 7.55 (d, J = 3.2 Hz, 1H), 7.70 (d, J = 8.7 Hz, 2H), 7.84–7.92 (m, 1H), 8.03–8.06 (m, 2H). ¹³C NMR (100 MHz, CDCl₃) δ 24.1, 25.9, 52.0, 54.3, 57.9, 119.0, 125.6, 126.0, 129.2, 130.8, 136.8, 141.9, 150.1, 166.5. HRMS (ESI) calculated for C₁₉H₂₃N₂O₃S ([M+H]⁺): 359.1429, found: 359.1425.

Yield: 51 mg, 33%. HPLC purity: 98.8%. ¹H NMR (400 MHz, CDCl₃) δ 1.40–1.44 (m, 2H), 1.56–1.60 (m, 4H), 2.44 (m, 4H), 3.67 (s, 2H), 6.87 (d, J = 3.7 Hz, 1H), 7.54 (d, J = 8.6 Hz, 2H), 7.59 (d, J = 3.7 Hz, 1H), 7.74 (d, J = 8.6 Hz, 2H), 8.27 (s, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 24.1, 25.9, 54.3, 57.9, 119.9, 122.7, 125.4, 125.9, 126.0, 126.2, 129.3, 136.9, 140.9, 150.3, 160.6. HRMS (ESI) calculated for C₁₈H₂₀F₃N₂OS ([M+H]⁺): 369.1248, found: 369.1242.

Yield: 66 mg, 47%. HPLC purity: 99%. ¹H NMR (400 MHz, CDCl₃) δ 1.46–1.47 (m, 2H), 1.58–1.63 (m, 4H), 2.40 (m, 4H), 3.16 (t, J = 8.3 Hz, 2H), 3.53 (s, 2H), 3.90 (s, 3H), 4.16 (t, J = 8.3 Hz, 2H), 7.42 (d, J = 8.1 Hz, 2H), 7.51 (d, J = 8.1 Hz, 2H), 7.86 (bs, 1H), 7.89 (s, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 29.1, 30.7, 32.4, 57.1, 59.1, 67.6, 129.8, 131.0, 132.1, 133.8, 134.2, 138.6, 140.1, 146.5, 151.9, 152.1, 171.0, 173.8. HRMS (ESI) calculated for C₂₃H₂₇N₂O₃ ([M+H]⁺): 379.2022, found: 379.2016.

Yield: 49 mg, 60%. HPLC purity: 99.8%. ¹H NMR (400 MHz, CDCl₃) δ 1.46–1.49 (m, 2H), 1.58–1.63 (m, 4H), 2.41 (m, 4H), 3.17 (t, J = 8.4, 2H), 3.54 (s, 2H), 4.15 (t, J = 8.4, 2H), 7.42–7.44 (m, 4H), 7.51 (d, J = 8.1 Hz, 2H). ¹³C NMR (100 MHz, CDCl₃) δ 24.2, 25.9, 27.8, 50.6, 50.9, 54.5, 63.4, 116.7, 121.9, 122.9, 124.9, 125.6, 127.0, 129.3, 133.0, 134.8, 141.8, 145.7, 169.4. HRMS (ESI) calculated for C₂₂H₂₄F₃N₂O ([M+H]⁺): 389.1841, found: 389.1836.

Yield: 54 mg, 66%. HPLC purity: 98%. ¹H NMR (400 MHz, DMSO-d₆) δ 1.40–1.48 (m, 2H), 1.60–1.65 (m, 4H), 2.44 (m, 4H), 3.18 (t, J = 8.3, 2H), 3.57 (s, 2H), 4.16 (t, J = 8.3, 2H), 7.28 (d, J = 8.4 Hz, 2H), 7.44 (d, J = 8.1 Hz, 2H), 7.51 (d, J = 8.3 Hz, 2H). ¹³C NMR (100 MHz, DMSO-d₆) δ 24.1, 25.7, 28.0, 50.7, 54.4, 63.2, 113.8, 120.8, 122.7, 125.0, 125.4, 127.0, 129.4, 129.9, 135.0, 136.2, 141.2, 143.3, 169.2; HRMS (ESI) calculated for C₂₂H₂₄F₃N₂O ([M+H]⁺): 389.1841, found: 389.1840.

Yield: 35 mg, 45%. HPLC purity: 99%. ¹H NMR (400 MHz, MeOD) δ 1.48–1.49 (m, 2H), 1.59–1.65 (m, 4H), 2.47 (m, 4H), 3.11 (t, J = 8.3 Hz, 2H), 3.59 (s, 2H), 4.10–4.14 (m, 2H), 7.01–7.03 (m, 1H), 7.16 (d, J = 8.4 Hz, 2H), 7.46 (d, J = 8.2 Hz, 1H), 7.52 (d, J = 8.2 Hz, 2H). ¹³C NMR (100 MHz, MeOD) δ 23.7, 25.1, 27.3, 54.0, 63.0, 124.0, 125.6, 126.8, 129.9, 131.1, 132.4, 135.2, 139.8, 169.6. HRMS (ESI) calculated for C₂₁H₂₄ClN₂O ([M+H]⁺): 355.1577, found: 355.1573.

Yield: 39 mg, 39%. HPLC purity: 99%. ¹H NMR (400 MHz, CDCl₃) δ 1.47–1.48 (m, 2H), 1.60–1.66 (m, 4H), 2.44 (m, 4H), 3.08 (t, J = 8.0 Hz, 2H), 3.57 (s, 2H), 4.13 (t, J = 8.0 Hz, 2H), 6.70 (m, 1H), 7.12 (t, J = 7.1 Hz, 1H), 7.44 (d, J = 7.0 Hz, 2H), 7.50 (d, J = 7.0 Hz, 2H). ¹³C NMR (100 MHz, CDCl₃) δ 24.1, 25.7, 27.5, 51.4, 54.4, 63.2, 105.4, 110.2, 125.2, 127.0, 127.5, 129.4, 135.2, 140.9, 143.9, 160.9, 163.3, 169.1. HRMS (ESI) calculated for C₂₁H₂₄FN₂O ([M+H]⁺): 339.1873, found: 339.1869.

Yield: 18 mg, 24%. HPLC purity: 99%. ¹H NMR (400 MHz, MeOD) δ 1.32 (c, 6H), 1.49–1.50 (m, 2H), 1.62–1.65 (m, 4H), 2.48 (m, 4H), 3.59 (s, 2H), 3.84 (s, 2H), 7.11–7.27 (m, 3H), 7.48 (d, J = 7.9 Hz, 2H), 7.55 (d, J = 7.8 Hz, 2H), 8.05 (m, 1H). ¹³C NMR (100 MHz, MeOD) δ 23.7, 25.1, 26.5, 54.0, 62.8, 121.9, 124.5, 126.8, 129.7, 135.5, 140.1, 140.9, 142.0, 169.5; HRMS (ESI) calculated for C₂₃H₂₉N₂O ([M+H]⁺): 349.2280, found: 349.2280.

Yield: 29 mg, 39%. HPLC purity: 99%. ¹H NMR (400 MHz, MeOD) δ 1.06 (m, 4H), 1.49–1.50 (m, 2H), 1.60–1.65 (m, 4H), 2.47 (m, 4H), 3.58 (s, 2H), 4.06 (s, 2H), 6.78 (d, J = 8.3 Hz, 1H), 7.04–7.13 (m, 2H), 7.47 (d, J = 8.1 Hz, 2H), 7.54 (d, J = 8.1 Hz, 2H), 8.20 (m, 1H). ¹³C NMR (100 MHz, MeOD) δ 15.8, 22.4, 23.7, 25.1, 53.9, 58.7, 62.8, 116.7, 118.4, 124.5, 126.1, 126.6, 129.7, 135.7, 137.8, 139.8, 142.6, 169.7. HRMS (ESI) calculated for C₂₃H₂₆N₂O ([M+H]⁺): 347.2123, found: 347.2120.

Yield: 34 mg, 42%. HPLC purity: 98%. ¹H NMR (400 MHz, CDCl₃) δ 1.43–1.44 (m, 5H), 1.61–1.62 (m, 4H), 2.43–2.49 (m, 4H), 3.18 (t, J = 8.2 Hz, 2H), 3.52 (m, 1H), 4.18 (t, J = 8.1 Hz, 2H), 7.28 (d, J = 7.0 Hz, 1H), 7.44 (d, J = 8.1 Hz, 2H), 7.51 (d, J = 8.1 Hz, 2H). ¹³C NMR (100 MHz, CDCl₃) δ 19.2, 24.4, 26.0, 28.1, 29.8, 51.8, 65.5, 121.6, 125.8, 127.8, 128.9, 130.4, 135.7, 137.1, 144.1, 170.2. HRMS (ESI) calculated for C₂₃H₂₆F₃N₂O ([M+H]⁺): 403.1997, found: 403.1993.

Yield: 31 mg, 40%. HPLC purity: 98%. ¹H NMR (400 MHz, CDCl₃) δ 1.31 (s, 9H), 1.42–1.44 (m, 5H), 1.58–1.63 (m, 4H), 2.38–2.46 (m, 4H), 3.12 (t, J = 8.2 Hz, 2H), 3.48–3.51 (m, 2H), 4.11 (bs, 2H), 7.26 (d, J = 8.9 Hz, 1H), 7.40 (d, J = 8.1 Hz, 2H), 7.50 (d, J = 8.1 Hz, 2H), 8.2 (bs, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 19.0, 24.4, 26.0, 28.4, 31.5, 34.4, 50.6, 51.4, 65.0, 121.8, 124.1, 127.1, 127.9, 132.1, 135.6, 145.8, 168.8. HRMS (ESI) calculated for C₂₆H₃₅N₂O ([M+H]⁺): 391.2749, found: 391.2743.

Yield: 40 mg, 51%. HPLC purity: 98%. ¹H NMR (400 MHz, CDCl₃) δ 0.95 (s, 9H), 1.43–1.44 (m, 5H), 1.58–1.62 (m, 4H), 2.41–2.46 (m, 4H), 2.94–2.97 (m, 1H), 3.50 (quintet, J = 6.4 Hz, 2H), 4.07 (t, J = 10.0 Hz, 2H), 7.08 (m, 2H), 7.28 (d, J = 4.6 Hz, 1H), 7.41 (d, J = 7.8 Hz, 2H), 7.49 (d, J = 7.8 Hz, 2H). ¹³C NMR (100 MHz, CDCl₃) δ 18.8, 18.9, 24.4, 26.0, 26.9, 34.3, 50.3, 51.4, 64.9, 123.1, 127.0, 127.5, 128.0, 134.0, 135.3, 143.2, 146.0, 168.4. HRMS (ESI) calculated for C₂₆H₃₅N₂O ([M+H]⁺): 391.2749, found: 391.2748.

Yield: 34 mg, 44%. HPLC purity: 99%. ¹H NMR (400 MHz, CDCl₃) δ 1.41–1.42 (m, 2H), 1.52–1.58 (m, 4H), 2.37 (m, 4H), 3.50 (s, 2H), 7.39 (d, J = 8.2 Hz, 2H), 7.64 (d, J = 8.6 Hz, 1H), 7.85 (d, J = 8.2 Hz, 2H), 8.57 (dd, J = 2.2, 8.6 Hz, 1H), 8.76 (d, J = 2.2 Hz, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 23.9, 25.3, 54.4, 63.3, 120.9, 127.5, 127.8, 129.7, 132.7, 138.3, 141.3, 142.2, 142.5, 167.1. HRMS (ESI) calculated for C₁₉H₂₁F₃N₃O ([M+H]⁺): 364.1637, found: 364.1642.

Yield: 24 mg, 17%. HPLC purity: 98%. ¹H NMR (400 MHz, CDCl₃) δ 1.38–1.44 (m, 2H), 1.55–1.59 (m, 4H), 2.03–2.09 (m, 2H), 2.35 (m, 4H), 2.90 (t, J = 6.5 Hz, 2H), 3.46 (s, 2H), 3.88 (s, 3H), 3.92 (t, J = 6.5 Hz, 2H), 6.81 (d, J = 8.5 Hz, 1H), 7.25 (d, J = 8.1 Hz, 2H), 7.32 (d, J = 8.1 Hz, 2H), 7.52 (dd, J = 1.6, 8.4 Hz, 1H), 7.85 (s, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 23.7, 24.2, 25.8, 27.0, 44.9, 51.9, 54.4, 63.2, 124.8, 125.5, 127.0, 128.5, 128.8, 129.3, 130.0, 130.6, 134.2, 143.6, 166.6, 170.6. HRMS (ESI) calculated for C₂₄H₂₉N₂O₃ ([M+H]⁺): 393.2178, found: 393.2175.

Yield: 4 mg, 5%. HPLC purity: 95%. ¹H NMR (400 MHz, MeOD) δ 1.51–1.52 (m, 2H), 1.62–1.68 (m, 4H), 2.51 (m, 4H), 3.66 (d, 2H), 6.81 (d, J = 3.8 Hz, 1H), 7.59–7.63 (m, 4H), 7.76 (d, J = 8.0 Hz, 2H), 7.81 (d, J = 8.2 Hz, 1H), 8.64 (s, 1H). ¹³C NMR (100 MHz, MeOD) δ 23.6, 25.1, 54.0, 62.7, 107.7, 113.0, 120.0, 121.3, 129.0, 129.6, 130.4, 133.4, 135.1, 142.5, 167.1. HRMS (ESI) calculated for C₂₂H₂₂FN₂O ([M+H]⁺): 387.1684, found: 387.1689.

Yield: 27 mg, 34%. HPLC purity: 99.6%. ¹H NMR (400 MHz, CDCl₃) δ 1.44–1.47 (m, 2H), 1.58–1.63 (m, 4H), 2.46 (m, 4H), 3.25 (t, J = 8.4 Hz, 2H), 3.70 (s, 2H), 3.90 (s, 3H), 4.44 (t, J = 8.4 Hz, 2H), 6.91 (d, J = 3.7 Hz, 1H), 7.49 (d, J = 3.7 Hz, 1H), 7.88 (s, 1H), 7.92 (d, J = 8.4 Hz, 1H), 7.78 (d, J = 8.4 Hz, 1H). ¹³C NMR (100 MHz, CDCl₃) δ 24.1, 25.9, 28.2, 50.9, 51.9, 54.3, 57.9, 117.0, 125.6, 126.0, 129.9, 130.6, 131.9, 137.2, 147.3, 149.2, 162.0, 166.7. HRMS (ESI) calculated for C₂₁H₂₅N₂O₃S ([M+H]⁺): 358.1586, found: 358.1588.