Incomplete influenza A virus genomes occur frequently but are readily complemented during localized viral spread

Jacobs, Nathan T.; Onuoha, Nina O.; Antia, Alice; Steel, John; Antia, Rustom; Lowen, Anice C.

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CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners. As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.

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Incomplete influenza A virus genomes occur frequently but are readily complemented during localized viral spread

August 06 2019
By Jacobs, Nathan T. ; Onuoha, Nina O. ; Antia, Alice ; ...
Source: Nat Commun. 10

[PDF-1.34 MB]

English

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Alternative Title:

Nat Commun
Personal Author:

Jacobs, Nathan T. ; Onuoha, Nina O. ; Antia, Alice ; Steel, John ; Antia, Rustom ; ... More +

Jacobs, Nathan T. ; Onuoha, Nina O. ; Antia, Alice ; Steel, John ; Antia, Rustom ; Lowen, Anice C. Less -
Description:

Segmentation of viral genomes into multiple RNAs creates the potential for replication of incomplete viral genomes (IVGs). Here we use a single-cell approach to quantify influenza A virus IVGs and examine their fitness implications. We find that each segment of influenza A/Panama/2007/99 (H3N2) virus has a 58% probability of being replicated in a cell infected with a single virion. Theoretical methods predict that IVGs carry high costs in a well-mixed system, as 3.6 virions are required for replication of a full genome. Spatial structure is predicted to mitigate these costs, however, and experimental manipulations of spatial structure indicate that local spread facilitates complementation. A virus entirely dependent on co-infection was used to assess relevance of IVGs in vivo. This virus grows robustly in guinea pigs, but is less infectious and does not transmit. Thus, co-infection allows IVGs to contribute to within-host spread, but complete genomes may be critical for transmission.
Subjects:

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Animals Defective Viruses Disease Models, Animal Dogs Evolution, Molecular Female Genome, Viral Guinea Pigs HEK293 Cells Humans Influenza, Human Influenza A Virus, H3N2 Subtype Likelihood Functions Madin Darby Canine Kidney Cells Models, Biological RNA, Viral Single-Cell Analysis Viral Load Virion Virus Replication Virus Shedding
Source:

Nat Commun. 10
Pubmed ID:

31387995
Pubmed Central ID:

PMC6684657
Document Type:

Journal Article
Funding:

R01 AI099000/AI/NIAID NIH HHS/United States ; U19 AI117891/AI/NIAID NIH HHS/United States ; HHSN272201400004C/AI/NIAID NIH HHS/United States

R01 AI099000/AI/NIAID NIH HHS/United States ; U19 AI117891/AI/NIAID NIH HHS/United States ; HHSN272201400004C/AI/NIAID NIH HHS/United States Less -
Volume:

10
Collection(s):

CDC Public Access
Main Document Checksum:

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urn:sha256:b6b012b32be0db3f5f4270ddf805fc0b70ab0087a4a205e206e1f746a197e5c1
Download URL:

https://stacks.cdc.gov/view/cdc/89359/cdc_89359_DS1.pdf
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