Lineage-Specific Real-Time RT-PCR for Yellow Fever Virus Outbreak Surveillance, Brazil

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Fischer, Carlo ; Torres, Maria C. ; Patel, Pranav ; Moreira-Soto, Andres ; Gould, Ernest A. ; Charrel, Rémi N. ; de Lamballerie, Xavier ; Nogueira, Rita Maria Ribeiro ; Sequeira, Patricia C. ; Rodrigues, Cintia D.S. ; Kümmerer, Beate M. ; Drosten, Christian ; Landt, Olfert ; Bispo de Filippis, Ana Maria ; Drexler, Jan Felix
• Description:
  The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We developed novel multiplex real-time reverse transcription PCRs that differentiate between vaccine and American wild-type YFV. We validated these highly specific and sensitive assays in an outbreak setting.
• Subjects:
  Disease Outbreaks Humans Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Species Specificity Yellow Fever Yellow Fever Vaccine Yellow Fever Virus
• Source:
  Emerg Infect Dis. 23(11):1867-1871.
• Pubmed ID:
  28949285
• Pubmed Central ID:
  PMC5652426
• Document Type:
  Journal Article
• Genre:
  Dispatch
• Place as Subject:
  Brazil
• Volume:
  23
• Issue:
  11
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:3775feeb938f3c304db6b9f0e579bb643f93610acb7ffef0d2f5d0aae4022582
• Download URL:
  https://stacks.cdc.gov/view/cdc/49595/cdc_49595_DS1.pdf
• File Type:
  [PDF - 1.63 MB ]