PLoS OneplosplosonePLoS ONE1932-6203Public Library of ScienceSan Francisco, USA219698703182181PONE-D-11-1090110.1371/journal.pone.0025127Research ArticleBiologyMicrobiologyVirologyAntiviralsMedicineClinical Research DesignClinical TrialsPhase IIIDrugs and DevicesBehavioral PharmacologyDrug DependenceRecreational Drug UseInfectious DiseasesViral DiseasesHIVHIV epidemiologyHIV preventionMental HealthPsychiatrySubstance AbusePublic HealthPreventive MedicineEnrollment Characteristics and Risk Behaviors of Injection Drug Users Participating in the Bangkok Tenofovir Study, ThailandBangkok Tenofovir Study ParticipantsMartinMichael12*VanichseniSuphak3SuntharasamaiPravan3SangkumUdomsak3ChuachoowongRutt1MockPhilip A.1LeethochawalitManoj4ChiamwongpaetSithisat4KittimunkongSomyot5van GriensvenFrits1McNichollJanet M.12PaxtonLynn2ChoopanyaKachit3for the Bangkok Tenofovir Study GroupThailand Ministry of Public Health – US Centers for Disease Control and Prevention Collaboration, Nonthaburi, ThailandCenters for Disease Control and Prevention, Atlanta, Georgia, United States of AmericaBangkok Tenofovir Study Group, Bangkok, ThailandBangkok Metropolitan Administration, Bangkok, ThailandThailand Ministry of Public Health, Nonthaburi, ThailandVermundSten H.EditorVanderbilt University, United States of America* E-mail: Znd9@cdc.gov

Conceived and designed the experiments: MM SV PS US RC PAM ML SC SK FvG JMM LP KC. Performed the experiments: MM SV PS US RC FvG JMM KC. Analyzed the data: MM PAM PS. Contributed reagents/materials/analysis tools: MM PAM ML SC KC. Wrote the paper: MM SV PS US RC PAM ML SC SK FvG JMM LP KC.

¶ Membership of the Bangkok Tenofovir Study Group is provided in the Acknowledgments.

2011289201169e2512721620112582011This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.2011Background

The Bangkok Tenofovir Study was launched in 2005 to determine if pre-exposure prophylaxis with tenofovir will reduce the risk of HIV infection among injecting drug users (IDUs). We describe recruitment, screening, enrollment, and baseline characteristics of study participants and contrast risk behavior of Tenofovir Study participants with participants in the 1999–2003 AIDSVAX B/E Vaccine Trial.

Methods

The Bangkok Tenofovir Study is an ongoing, phase-3, randomized, double-blind, placebo-controlled, HIV pre-exposure prophylaxis trial of daily oral tenofovir. The Tenofovir Study and the Vaccine Trial were conducted among IDUs at 17 drug-treatment clinics in Bangkok. Tenofovir Study sample size was based on HIV incidence in the Vaccine Trial. Standardized questionnaires were used to collect demographic, risk behavior, and incarceration data. The Tenofovir Study is registered with ClinicalTrials.gov, number-NCT00119106.

Results

From June 2005 through July 2010, 4094 IDUs were screened and 2413 enrolled in the Bangkok Tenofovir Study. The median age of enrolled participants was 31 years (range, 20–59), 80% were male, and 63% reported they injected drugs during the 3 months before enrollment. Among those who injected, 53% injected methamphetamine, 37% midazolam, and 35% heroin. Tenofovir Study participants were less likely to inject drugs, inject daily, or share needles (all, p<0.001) than Vaccine Trial participants.

Discussion

The Bangkok Tenofovir Study has been successfully launched and is fully enrolled. Study participants are significantly less likely to report injecting drugs and sharing needles than participants in the 1999–2003 AIDSVAX B/E Vaccine Trial suggesting HIV incidence will be lower than expected. In response, the Bangkok Tenofovir Study enrollment was increased from 1600 to 2400 and the study design was changed from a defined 1-year follow-up period to an endpoint-driven design. Trial results demonstrating whether or not daily oral tenofovir reduces the risk of HIV infection among IDUs are expected in 2012.

Introduction

HIV spread rapidly among injecting drug users (IDUs) in Bangkok in the late 1980s [1] and HIV prevalence has remained high, 30% to 50% [2], [3]. Safe and effective tools to prevent HIV infection among IDUs are urgently needed. Use of antiretroviral drugs before HIV exposure (pre-exposure prophylaxis) may protect people at high risk of HIV from infection and provide a new tool to reduce HIV transmission.

The Bangkok Tenofovir Study is an ongoing phase-3, randomized, double-blind, placebo-controlled, endpoint-driven HIV prevention trial that aims to determine if daily oral Tenofovir Disoproxil Fumarate (tenofovir) will reduce HIV transmission among IDUs. Tenofovir, a nucleotide reverse transcriptase inhibitor, is a potent antiretroviral with a long half-life allowing once-daily dosing [4]–[7]. Data from clinical trials among people infected with HIV have shown that tenofovir has a good safety profile [8], [9], a low potential to select for tenofovir resistance [10], [11], and co-administration of tenofovir with methadone and/or oral contraceptives does not alter the pharmacokinetics or pharmacodynamics of these drugs [12], [13]. Tenofovir was licensed for the treatment of HIV infection by the US Food and Drug Administration in 2001 and the Thailand Food and Drug Administration in 2006.

Several lines of evidence suggest that pre-exposure prophylaxis with tenofovir will reduce HIV transmission among IDUs. Studies of macaques have shown that tenofovir can prevent or delay infection with simian immunodeficiency virus and humanized derivatives called SHIV [14]–[17] and the use of antiretroviral drugs reduces the risk that HIV-infected pregnant women will transmit HIV to their newborns [18] and that health care workers will become infected following occupational exposures to HIV [19], [20].

Following consultations with IDUs and representatives of organizations working with communities at risk for HIV infection, we began preparations for an HIV pre-exposure prophylaxis trial in 2004 [21]. The findings of two longitudinal studies conducted among IDUs in Bangkok informed the design of the study. The first was a preparatory cohort study [22] that enrolled 1209 IDUs at drug-treatment clinics managed by the Bangkok Metropolitan Administration (BMA), the city government of Bangkok, during 1995–1996 and followed them for 3 years. The second study was the 1999–2003 AIDSVAX B/E Vaccine Trial [23] conducted among 2546 IDUs in the same BMA drug-treatment clinics. The vaccine did not prevent HIV infection, but IDUs showed a continued willingness to participate in research and 90% completed the study. HIV incidence remained stable during follow-up at 3.4 per 100 person-years.

Based on community interest in promising HIV prevention interventions, ongoing high HIV incidence among IDUs [23], and evidence suggesting pre-exposure prophylaxis with tenofovir would prevent HIV infection [14]–[20], a protocol to evaluate tenofovir among IDUs was developed and submitted for regulatory review. Regulatory approvals were obtained in May 2005 and the Bangkok Tenofovir Study was launched in June 2005.

Since the study began several other pre-exposure prophylaxis trials have provided results that support the rationale for the Bangkok Tenofovir Study. A trial among women in South Africa found use of tenofovir vaginal gel reduced HIV acquisition by 39% [24] and a trial among men who have sex with men found use of daily oral truvada (tenofovir+emtricitabine) reduced HIV incidence by 44% [25]. Although a trial comparing daily oral truvada to placebo among women in several African countries was stopped in 2010 because interim data showed that it was unlikely the study would demonstrate lower HIV infection rates among women receiving truvada [26], a trial among heterosexual men and women in Botswana found that participants randomized to receive daily truvada were 63% less likely to become HIV infected than participants receiving placebo [27] and a trial among heterosexual HIV serodiscordant couples in Kenya and Uganda found that daily tenofovir reduced HIV acquisition 62% and daily truvada 73% compared to placebo [28].

Overall, these studies provide evidence that pre-exposure prophylaxis with tenofovir or truvada is safe and can reduce the risk of sexual transmission of HIV among heterosexual couples and men who have sex with men. The results do not provide information about the efficacy of tenofovir or truvada to prevent parenteral transmission of HIV among IDUs. In order to determine if daily oral tenofovir can reduce the risk of HIV infection among IDUs, we are moving forward to complete the Bangkok Tenofovir Study.

The research team became aware during the first year of the study that participant risk behavior differed from Vaccine Trial participants, suggesting HIV incidence would be lower than expected. Here, we describe Bangkok Tenofovir Study recruitment, screening, and enrollment, and contrast risk behavior of Tenofovir Study participants with Vaccine Trial participants. These findings led the research team to increase Bangkok Tenofovir Study enrollment and change to an endpoint-driven study design.

Methods

The protocol for this trial and supporting CONSORT checklist are available as supporting information; see Checklist S1 and Protocol S1.

Community engagement

In preparation for the study, the research team met with IDUs and representatives of organizations working with communities at risk for HIV infection, to describe the project; distribute draft protocols, consent forms, and education materials; and to gather input for trial materials and procedures. Focus group discussions were conducted to assess IDU willingness to join an HIV pre-exposure prophylaxis study, understanding of clinical trial design and procedures, and concerns about the use of tenofovir. A community relations committee made up of at least one IDU from each of the 17 BMA clinics was formed to provide ongoing community input during the trial. The committee meets with the research team every 2 months to discuss a broad range of issues that impact IDUs, clinic staff, and the study.

Study setting and design

The study is being conducted at 17 BMA drug-treatment clinics in the densely populated urban communities of Bangkok. The clinics offer a range of services including HIV counseling and testing, risk-reduction counseling, social and welfare services, health education, primary medical care and referrals, methadone treatment, condoms, and bleach to clean injection equipment with demonstrations of appropriate use. These services are free of charge. Thailand's narcotics law prohibits the distribution of needles to inject illicit drugs and needles are not provided in the clinics; however, sterile needles and syringes are available to the public over the counter at low cost (5 to 10 baht/0.12 to 0.25 USD) in pharmacies in Bangkok.

We based sample size calculations for the Tenofovir Study on HIV incidence among IDUs in the 1999–2003 Vaccine Trial [23]. We estimated the efficacy of tenofovir to prevent HIV infection was 67% and designed the study to have 80% power to demonstrate at least 10% tenofovir efficacy with a one-sided type 1 error of 2.5%. In order to meet these criteria, we planned to enroll 1600 participants and follow each participant for 12 months. During the first year of the Tenofovir Study we discovered that participant reports of injecting and needle sharing were less than observed in the Vaccine Trial, suggesting HIV incidence would be lower than expected. Based on the original Tenofovir Study design, a lower than expected HIV incidence would have resulted in fewer endpoints (i.e., incident HIV infections), a larger confidence interval around the efficacy estimate, and a diminished likelihood the trial would determine if daily oral tenofovir reduced the risk of HIV infection among IDUs. In response, the Bangkok Tenofovir Study enrollment target was increased from 1600 to 2400 and the study design was changed from a defined 1-year follow-up period to an endpoint (incident HIV infection)-driven design. These changes provide 82% power to demonstrate at least 10% tenofovir efficacy with a one-sided type 1 error of 2.5%.

Regulatory review

The study protocol, informed consent documents, questionnaires, and education materials were reviewed and approved by the BMA Ethical Review Committee, the Thailand Ministry of Public Health Ethical Review Committee, and an Institutional Review Board of the US Centers for Disease Control and Prevention. The US Office for Human Research Protections approved the follow-up of incarcerated participants.

An independent Data and Safety Monitoring Board conducts annual safety reviews and one interim efficacy review. A clinical research organization is employed to provide independent oversight and assure compliance with international guidelines for good clinical practice. Gilead (Gilead Sciences, Inc., Foster City, California) provides tenofovir and placebo free of charge but has not been involved in the design or conduct of the study, data analysis, or the presentation of results.

Recruitment

Research staff placed posters and brochures describing the study in the drug-treatment clinics, provided presentations about the study at IDU drop-in centers, and were available at the clinics to discuss the study with individuals interested in joining the trial. Potential participants received an explanation of study objectives and design, eligibility criteria, and study activities and procedures.

Eligibility evaluation

HIV-uninfected individuals aged 20 to 60 years who reported injecting drugs during the previous 12 months were candidates for the study. Table 1 lists eligibility criteria. We excluded people with chronic HBV infection because of concerns about reactivation (i.e., flares) of hepatic disease if use of tenofovir was stopped. HBV vaccine is provided to enrolled participants who have no serologic evidence of active or chronic Hepatitis B infection. We excluded women who were pregnant or breast feeding and required women to agree to abstain from sexual intercourse or use contraception (i.e., oral, injection, or barrier) during the study because large well-controlled studies of tenofovir had not been conducted among pregnant or breast-feeding women. Contraceptives are provided to participants free of charge.

10.1371/journal.pone.0025127.t001Eligibility requirements for the Bangkok Tenofovir Study.
In order to enroll potential participants must:
•Be 20 to 60 years-old
•Report injection drug use in the 12 months before screening
•Possess documentation of a Thai National Identification number
Have the following laboratory results from a blood or oral fluid specimen collected in the 2 weeks before enrollment:
•A non-reactive HIV oral fluid test
•Hemoglobin ≥9 gm/dL
•Alanine aminotransferase (ALT) ≤102 U/L
•Aspartate aminotransferase (AST) ≤95 U/L
•Total bilirubin ≤1.5 mg/dL
•Amylase ≤144 U/L
•Phosphorus ≥2.2 mg/dL
•Negative hepatitis B surface antigen
•Calculated creatinine clearance ≥60 mL/min by the Cockcroft-Gault formula where creatinine clearance in mL/min = Male: (140−age in years)×(wt in kg)/72×(serum creatinine in mg/dL)Female: (140−age in years)×(wt in kg)×0.85/72×(serum creatinine in mg/dL)
•Pass the Bangkok Tenofovir Study comprehension test
•Be willing and able to provide informed consent for study participation
•Be available and committed to daily or monthly follow-up for at least 12 months
In addition:
•Women must not be pregnant or breastfeeding and must be willing to abstain from sexual intercourse or use contraception during the trial (i.e., oral, injection, or barrier)
•A volunteer may be excluded if s/he has a history of significant renal, liver, or bone disease or other clinical condition or prior therapy that, in the judgment of the study physician, would make the subject unsuitable for the study
•A volunteer will be excluded if s/he is participating in another HIV prevention, drug, or vaccine trial

Eligible volunteers completed a comprehension test to assess understanding of key trial concepts. Volunteers meeting all eligibility criteria were enrolled after providing written informed consent.

Enrollment and randomization

Enrolled participants were randomly assigned in a 1∶1 ratio to receive daily oral tenofovir 300 mg or placebo. The randomization list, created using a computerized random-number generator, was shared with Gilead who prepared and labeled bottles with a randomization number. When an eligible participant completed the consent process, study staff assigned them the next sequential randomization number. Participants, study staff, monitors, and other staff involved in the trial are blinded to drug assignment for the duration of the study.

Research staff collected baseline demographic and incarceration information using interviewer-administered questionnaires. Participants chose daily clinic visits with directly observed taking of study drug (DOT) or monthly visits without DOT. At enrollment and each monthly follow-up visit, participants are assessed for adverse events, oral fluid is collected for HIV testing (OraQuick Rapid HIV-1/2 Antibody Test, assembled in Thailand for Orasure Technologies, Inc, Bethlehem, Pennsylvania) and urine for pregnancy testing (OneStep urine test, ULTI Med Products, Ahrensburg, Germany); adherence is assessed using an audio computer-assisted self-interview (ACASI) and pill counts; and adherence and HIV risk-reduction counseling are provided. Reactive oral fluid HIV tests are confirmed using two different enzyme-immunoassays on blood (EIA) (Genetic Systems HIV-1/HIV-2 EIA, Washington, USA) and Western blot (Bio-Rad, Redmond, Washington).

At enrollment, months 1, 2, 3, and every 3 months thereafter, blood is collected for hematologic, hepatic, and renal safety assessment. Participants complete a risk questionnaire assessing drug use, incarceration, and sexual activity during the previous 3 months using an ACASI at enrollment and every 3 months thereafter.

To compensate participants for their time, effort, and travel, they receive 350 baht (∼10 US dollars) for each monthly study visit. Participants on the DOT schedule receive 70 baht (∼2 US dollars) each day they come to clinic and participants who come all 7 days in a week receive 350 baht for that week.

AIDSVAX B/E Vaccine Trial

The AIDSVAX B/E Vaccine Trial was a phase-3, randomized, double-blind, placebo-controlled study conducted among IDUs at BMA drug-treatment clinics during 1999–2003. HIV-uninfected individuals aged 20 to 60 years who reported injecting drugs during the previous 12 months were eligible for the trial. Pregnant or breastfeeding women were excluded. There were no blood chemistry or hematologic eligibility requirements. Detailed descriptions of the study have been published [23], [29]–[33]. Briefly, 2546 IDUs enrolled and were randomly assigned (1∶1) to receive either AIDSVAX B/E or placebo at months 0, 1, 6, 12, 18, 24, and 30. An interviewer-administered questionnaire was used at baseline and every 6 months to assess drug use, incarceration, and sexual activity during the previous 6 months.

Statistical analysis

Vaccine Trial and Tenofovir Study participant enrollment demographic and risk characteristics were compared using chi-square for categorical variables and t-test or Wilcoxon rank sum test for continuous variables. Adjusted odds ratios and 95% confidence intervals (CI) were estimated using logistic regression; factors significant (p<0.1) in univariate analyses were retained in the multivariable model. In order to evaluate injection of heroin, midazolam, and methamphetamine, we included these variables in the multivariable model and did not include ‘injected any drugs’.

We used SAS version 9.2 (SAS Institute, Cary, North Carolina) for statistical analyses.

ResultsBaseline characteristics

From June 2005 through July 2010, 4094 people were evaluated for enrollment (Figure 1). Their median age was 32 years (mean 33.3; range, 19–60), 82% were male and 11% were HIV infected. HIV infection was the most common reason screened volunteers were not able to enroll, followed by elevated AST or ALT (10%), and current or chronic hepatitis B infection (6%).

10.1371/journal.pone.0025127.g001Number of injection drug users screened and enrolled in the Bangkok Tenofovir Study in Bangkok, Thailand, 2005–2010.

Among the 2413 (59%) IDUs who enrolled, the median age was 31 years (mean 32.4; range, 20–59), 80% were male, 48% had a primary school (grade 6) or less education, and 22% were in a methadone treatment program (Table 2). Most participants reported they had been incarcerated in the past: 1905 (79%) in a police holding cell, 1403 (58%) in prison, and 1386 (57%) in both. Participants reporting incarceration in police holding cells had been incarcerated a median of 3 times (range, 1–60); in prisons a median of 2 times (range, 1–20).

10.1371/journal.pone.0025127.t002Results of bivariate and multivariate analysis comparing baseline demographic characteristics and risk activities reported by injection drug users participating in the 1999–2003 AIDSVAX B/E Vaccine Trial and the ongoing Bangkok Tenofovir Study, Thailand.
VaccineTenofovir
N = 2546N = 2413ORAdjusted OR
VariableNo. (%)No. (%)(95% CI)P value(95% CI)P value
SexMale2377 (93.4)1924 (79.7)0.3 (0.2–0.3)<0.0010.4 (0.3–0.5)<0.001
Female169 (6.6)489 (20.3)1.01.0
Age (years)Mean28.832.41.0 (1.0–1.1)<0.0011.1 (1.1–1.1)<0.001
Education≤Primary (year 6)833 (32.7)1154 (47.8)1.9 (1.7–2.1)<0.0011.1 (0.9–1.3)0.39
Secondary or more1713 (67.3)1259 (52.2)1.01.0
Ever been in jail (holding cell)Yes1943 (76.3)1905 (79.0)1.2 (1.0–1.3)0.031.3 (1.0–1.8)0.05
No603 (23.7)508 (21.0)1.01.0
Ever been in prisonYes1278 (50.2)1403 (58.1)1.4 (1.2–1.5)<0.0011.0 (0.8–1.2)0.86
No1268 (49.8)1010 (41.9)1.01.0
Reported risks*N = 2546N = 2405
In methadone treatmentYes2150 (84.4)523 (21.8)0.05 (0.04–0.06)<0.0010.1 (0.1–0.2)<0.001
No396 (15.6)1882 (78.2)1.0
Injected any drugsYes2389 (93.8)1506 (62.6)0.1 (0.1-0.1)<0.001Not included
No157 (6.2)899 (37.4)1.0
Injected heroinYes2355 (92.5)526 (21.9)0.02 (0.02–0.03)<0.0010.05 (0.04–0.06)<0.001
No191 (7.5)1879 (78.1)1.01.0
Injected methamphetamineYes388 (15.2)801 (33.3)2.8 (2.4–3.2)<0.0012.7 (2.1–3.3)<0.001
No2158 (84.8)1604 (66.7)1.01.0
Injected midazolamYes236 (9.3)558 (23.2)3.0 (2.5–3.5)<0.0017.7 (5.9–10.0)<0.001
No2310 (90.7)1847 (76.8)1.01.0
Injection frequencyDaily936 (36.8)204 (8.5)0.2 (0.1–0.2)<0.0010.5 (0.4–0.7)<0.001
<Daily1610 (63.2)2201 (91.5)1.01.0
Shared needlesYes790 (31.0)435 (18.1)0.5 (0.4–0.6)<0.0010.6 (0.5–0.8)<0.001
No1756 (69.0)1970 (81.9)1.01.0
In jail (police holding cell)Yes360 (14.1)552 (23.0)1.8 (1.6–2.1)<0.0011.4 (1.0–1.8)0.03
No2186 (85.9)1853 (77.0)1.0
In prisonYes188 (7.4)389 (16.2)2.4 (2.0–2.9)<0.0011.2 (0.8–1.6)0.45
No2358 (92.6)2016 (83.8)1.0
Sexual intercourse with more than one partnerYes306 (12.0)529 (22.0)2.1 (1.8–2.4)<0.0012.0 (1.5–2.5)<0.001
No2240 (88.0)1876 (78.0)1.01.0

Baseline risk behavior data were available on 2405 (99.7%) of enrolled participants. At enrollment, 1506 (63%) participants reported they had injected drugs and 435 (18%) had shared needles during the 3 months before enrollment. Among those who injected, 801 (53%) injected methamphetamine, 558 (37%) midazolam, 526 (35%) heroin, 124 (8%) other sedative-hypnotics, and 55 (4%) other drugs. A substantial proportion of those who injected drugs reported injecting more than one drug: 203 (14%) injected methamphetamine and midazolam, 186 (12%) methamphetamine and heroin, 184 (12%) heroin and midazolam, and 69 (5%) heroin, methamphetamine, and midazolam.

Among the 610 (25%) participants who reported incarceration during the 3 months before enrollment, 552 (90%) had been in a police holding cell, 389 (64%) in prison, and 331 (54%) in both. Of those who spent time in holding cells, 40 (7%) reported injecting drugs and 36 (6%) reported sexual intercourse in the cells. Of those who had been in prison, 33 (8%) reported injecting drugs and 35 (9%) reported sexual intercourse in prison.

At enrollment, 682 (28%) participants reported they had not had sexual intercourse with a same sex or opposite sex partner during the previous 3 months; 1194 (50%) reported intercourse with one partner; and 529 (22%) with more than one partner. Among enrolled participants, 1044 (43%) reported sexual intercourse with a partner with whom they lived, 78 (8%) using a condom every time; 913 (38%) reported intercourse with at least one casual (i.e., non-live-in) partner, 299 (37%) of the 806 male participants used a condom every time. A data entry problem limited assessment of condom use with casual partners to men. Among the 1916 male participants who completed a risk assessment at enrollment, 91 (5%) reported sexual intercourse with at least one male partner and 44 (48%) of these men used a condom every time. Most, 73 (80%), of these men also reported sexual intercourse with women.

Bivariate and multivariable analyses comparing baseline characteristics of Vaccine Trial and Tenofovir Study participants are shown in Table 2. Tenofovir Study participants are considerably less likely to inject drugs than Vaccine Trial participants (odds ratio 0.1, 95% CI 0.1-0.1, p<0.001). Multivariable analyses shows that Tenofovir Study participants are more likely to be female and older than Vaccine Trial participants (both, p≤0.001). Tenofovir Study participants are less likely to have injected heroin but more likely to have injected midazolam or methamphetamine than Vaccine Trial participants (all, p<0.001). Tenofovir Study participants are less likely to inject daily (p<0.001) or share needles (p = <0.001) but more likely to report sexual intercourse with more than one partner (p<0.001) than Vaccine Trial participants.

Discussion

The Bangkok Tenofovir Study, an ongoing HIV pre-exposure prophylaxis trial among IDUs, has been successfully launched and is fully enrolled. Participant reports of injection drug use and needle sharing are significantly less than expected, suggesting HIV incidence will be lower than incidence estimates used in the design of the study. To improve the likelihood the trial achieves its primary objective, to determine if daily oral tenofovir prevents HIV infection, we increased the enrollment target from 1600 to 2400 and changed participant follow-up from a defined 1-year follow-up period to an endpoint-driven design.

The lower level of risk behavior reported by Tenofovir Study participants compared to Vaccine Trial participants is likely due to several reasons. Risk-reduction counseling, methadone treatment, and other services offered by the BMA drug-treatment clinics may have reduced risk behavior among IDUs attending the clinics. Tenofovir Study eligibility requirements including blood chemistry and hematologic assessments and the exclusion of individuals with chronic hepatitis B infection may have led to the enrollment of a population of participants who inject drugs and share needles less frequently than participants in the Vaccine Trial. Changes in drugs injected by Vaccine Trial participants were recognized during the 1999–2003 trial [32], [33]. A decrease in heroin use, which is usually injected, and more methamphetamine and midazolam use, which can be taken orally or inhaled, may be contributing to lower levels of injection drug use and needle sharing. These changes coincided with the Thai Government's ‘War on Drugs’. The drug war was launched in 2003 to decrease the supply of illicit drugs [34], [35]. During 2003, the supply of heroin in Bangkok decreased and the price increased four-fold from approximately 2500 to 10,000 Thai baht (60 to 250 USD) per 1000 mg [32]. The price of methamphetamine and midazolam increased as well, but remained more affordable: methamphetamine 150–250 baht (4 to 6 USD) per tablet and midazolam 40–60 baht (1 to 1.5 USD) per tablet. The changes in drug use are likely due in part to the changes in drug supply and cost.

It has taken 5 years to enroll 2413 IDUs in the Tenofovir Study compared to 18 months to enroll 2546 IDUs in the Vaccine Trial. More stringent Tenofovir Study inclusion criteria likely contributed to slower enrollment. There is also evidence that the number of IDUs in Bangkok has decreased in recent years. Researchers using capture-recapture methodology estimated there were 36,600 active opiate users in Bangkok in 1991 [36]. A population assessment done in 2003 using respondent-driven sampling methodology estimated there were 3595 IDUs in Bangkok [37]. Each methodology has limitations but the 10-fold difference suggests the population of IDUs in Bangkok has decreased.

Incarceration is a common experience among IDUs in Bangkok [29] with almost 80% of study participants reporting a history of incarceration and 25% incarcerated during the 3 months before enrollment. Previous studies have demonstrated an association between incarceration and HIV infection [29], [33], [38] and information about this association has been included in participant risk-reduction counseling.

Tenofovir Study participants report modest levels of sexual activity, with 78% reporting no sexual intercourse or intercourse with only one partner during the 3 months before enrollment. Study participants are, however, more likely to report sexual intercourse than Vaccine Trial participants. This change in sexual behavior may be related to decreasing heroin use and increasing methamphetamine use, warranting additional research [39], [40]. Previous studies among IDUs in Bangkok found no association between sexual activity and HIV infection [22], [33]. It will be important to monitor participant sexual activity to see if this remains true.

There are a number of limitations to this analysis including the use of self-reports. Self-reporting of stigmatized or illegal behavior is problematic and under-reporting of these activities is possible [41]. At baseline, Vaccine Trial participants reported risk behaviors for the previous 6 months using a standardized interviewer-administered questionnaire, while Tenofovir Study participants reported risk behaviors for the previous 3 months using ACASI. Studies suggest that ACASI provides an acceptable and more accurate method of collecting health risk behavior data than face-to-face interviews [42]–[44]. Tenofovir Study participants may more accurately report risk behaviors because of the shorter recall period (3 months in the Tenofovir Study, 6 months in the Vaccine Trial) and the use of ACASI. On the other hand, Vaccine Trial participants had a longer time at risk and may have been more willing to report risk behavior that took place more than 3 months ago [42]. The use of different data collection tools and time frames limits the comparability of risk behavior data from the two trials.

The Bangkok Tenofovir Study is fully enrolled. The Data Safety and Monitoring Board has recommended trial continuation following annual safety reviews and an interim efficacy review in 2009. Trial results demonstrating whether or not daily oral tenofovir is safe to use among HIV-uninfected IDUs and if tenofovir reduces the risk of HIV infection are expected in 2012.

Supporting Information

CONSORT Checklist.

(DOC)

Click here for additional data file.

Trial Protocol.

(DOC)

Click here for additional data file.

We thank the study participants, clinic staff, and the members of the Bangkok Tenofovir Study Group.

The Bangkok Tenofovir Study Group

Principal Investigator: Kachit Choopanya; Clinic Coordination Team: Suphak Vanichseni, Pravan Suntharasamai, Udomsak Sangkum, Boonrawd Prasittipol; Bangkok Metropolitan Administration: Sithisat Chiamwongpaet, Kraichak Kaewnil, Montira Thongsari, Manoj Leethochawalit, La-Ong Srisuwanvilai, Rapeepan Anekvorapong, Kovit Yongvanitjit; Thailand Ministry of Public Health: Somyot Kittimunkong; Thailand Ministry of Public Health – US Centers for Disease Control and Prevention Collaboration: Pasakorn Akarasewi, Benjamaporn Chaipung, Nartlada Chantharojwong, Thitima Cherdtrakulkiat, Wannee Chonwattana, Rutt Chuachoowong, Pitthaya Disprayoon, Timothy Holtz, Wanna Leelawiwat, Robert Linkins, Michael Martin, Janet McNicholl, Philip Mock, Supawadee Na-Pompet, Tanarak Plipat, Boonyos Raengsakulrach, Pairote Tararut, Punneeporn Wasinrapee, Dararat Worrajittanon; US Centers for Disease Control and Prevention: Roman Gvetadze, Lynn Paxton.

Competing Interests: The authors have declared that no competing interests exist.

Funding: Sponsor (US Centers for Disease Control and Prevention) staff had a role in study design, the collection, analysis, and interpretation of data, writing the report, and in the decision to submit the paper for publication.

References WenigerBGLimpakarnjanaratKUngchusakKThanprasertsukSChoopanyaK 1991 The epidemiology of HIV infection and AIDS in Thailand. AIDS 5 Suppl. 2 S71 S85 1845063 National AIDS Prevention and Alleviation Committee UNGASS Country Progress Report, Thailand. Thailand Ministry of Public Health, Department of Disease Control. Available: http://data.unaids.org/pub/report/2008/thailand_2008_country_progress_report_en.pdf Accessed 2011 May 26 WHO Epidemiological Fact Sheet on HIV and AIDS, Thailand, 2008 update. Available: http://apps.who.int/globalatlas/predefinedReports/EFS2008/full/EFS2008_TH.pdf Accessed 2011 May 26 De ClercqESakumaTBabaMPauwelsRBalzariniJ 1987 Antiviral activity of phosphonylmethoxyalkyl derivatives of purine and pyrimidines. Antiviral Res 8 261 272 3451698 De ClercqE 2003 Clinical Potential of the Acyclic Nucleoside Phosphonates Cidofovir, Adefovir, and Tenofovir in Treatment of DNA Virus and Retrovirus Infections. Clin Microbiol Rev 16 569 596 14557287 GallantJEDeresinskiS 2003 Tenofovir disoproxil fumarate. Clin Infect Dis 37 944 950 13130407 Gilead Sciences Viread (tenofovir disoproxil fumarate) tablets [full prescribing information] 2010 Foster City, California Gilead Sciences Available: http://www.gilead.com/pdf/viread_pi.pdf. Accessed 2011 May 26 GallantJEStaszewskiSPozniakALDeJesusESuleimanJM 2004 Efficacy and safety of tenofovir DF vs stavudine in combination therapy in antiretroviral-naïve patients: a 3-year randomized trial. JAMA 292 191 201 15249568 GallantJEDeJesusEArribasJRPozniakALGazzardB 2006 Tenofovir DF, emtricitabine, and efavirenz vs. zidovudine, lamivudine, and efavirenz for HIV. N Engl J Med 354 251 260 16421366 WainbergMAMillerMDQuanYSalomonHMulatoAS 1999 In vitro selection and characterization of HIV-1 with reduced susceptibility to PMPA. Antivir Ther 4 87 94 10682153 MargotNAEnejosaJChengAKMillerMDMcCollDJ 2009 Development of HIV-1 drug resistance through 144 weeks in antiretroviral-naïve subjects on emtricitabine, tenofovir disoproxil fumarate, and efavirenz compared with lamivudine/zidovudine and efavirenz in study GS-01-934. J Acquir Immune Defic Syndr 52 209 21 19644384 KearneyBPIsaacsonESatreJChengAK 2003 Tenofovir DF and oral contraceptives: Lack of a pharmacokinetic drug interaction [Abstract A-1618]. Intersci Conf Antimicrob Agents Chemother. Available: http://gateway.nlm.nih.gov/MeetingAbstracts/ma?f=102265007.html. Accessed 2011 August 26 SmithPKearneyBPLiawSCloenDBullockJM 2004 Effect of Tenofovir Disoproxil Fumarate on the Pharmacokinetics and Pharmacodynamics of Total, R-, and S-Methadone. Pharmacotherapy 24 970 977 15338845 TsaiCCFollisKESaboABeckTWGrantRF 1995 Prevention of SIV infection in macaques by (R)-9-(2-phosphonylmethoxypropyl) adenine. Science 270 1197 1199 7502044 SubbaraoSOttenRARamosAKimCJacksonE 2006 Chemoprophylaxis with tenofovir disoproxil fumarate provided partial protection against infection with simian human immunodeficiency virus in macaques given multiple virus challenges. J Infect Dis 194 904 911 16960777 van RompayKKAKearneyBPSextonJJColonRLawsonJR 2006 Evaluation of oral tenofovir disoproxil fumarate and topical tenofovir GS-7340 to protect infant macaques against repeated oral challenges with virulent simian immunodeficiency virus. J Acquir Immune Defic Syndr 43 6 14 16810108 García-LermaJGOttenRAQariSHJacksonECongM-e 2008 Prevention of Rectal SHIV Transmission in Macaques by Daily or Intermittent Prophylaxis with Emtricitabine and Tenofovir. PLoS Med 5 e28 doi:10.1371/journal.pmed.0050028 18254653 Panel on Treatment of HIV-Infected Pregnant Women and Prevention of Perinatal Transmission. 1 117 Recommendations for Use of Antiretroviral Drugs in Pregnant HIV-1-Infected Women for Maternal Health and Interventions to Reduce Perinatal HIV Transmission in the United States. May 24, 2010 Available: http://www.aidsinfo.nih.gov/contentfiles/PerinatalGL.pdf Accessed 2011 May 26 CardoDMCulverDHCiesielskiCASrivastavaPUMarcusR 1997 A case-control study of HIV seroconversion in health care workers after percutaneous exposure. N Engl J Med 337 1485 90 9366579 CDC 2005 Updated U.S. Public Health Service Guidelines for the Management of Occupational Exposures to HIV and Recommendations for Postexposure Prophylaxis. MMWR 54 RR-9 1 17 ChuachoowongRMartinMPrasitipholBVanichseniSSuntharasamaiP 2008 Involving communities to inform an HIV prevention trial in Bangkok [Abstract WEPE0262]. The 17th International AIDS Conference 2008, Mexico City. Available: http://www.aids2008.org/Pag/Abstracts.aspx?AID=4362. Accessed 2011 May 26 VanichseniSKitayapornDMastroTDMockPARakthamS 2001 Continued high HIV­1 incidence in a vaccine trial preparatory cohort of injection drug users in Bangkok, Thailand. AIDS 15 397 405 11273220 PitisuttithumPGilbertPGurwithMHeywardWMartinM 2006 Randomized, double-blind, placebo-controlled efficacy trial of a bivalent recombinant glycoprotein 120 HIV-1 vaccine among injection drug users in Bangkok, Thailand. J Infect Dis 194 1661 71 17109337 KarimQAKarimSSAFrohlichJAGroblerACBaxterC 2010 Effectiveness and Safety of Tenofovir Gel, an Antiretroviral Microbicide, for the Prevention of HIV Infection in Women. Science 326 1168 1174 GrantRMLamaJRAndersonPLMcMahanVLiuAY 2010 Preexposure Chemoprophylaxis for HIV Prevention in Men Who Have Sex with Men. NEJM 363 2587 99 21091279 FHI FEM-PrEP Project. Available: http://www.fhi360.org/en/Research/Projects/FEM-PrEP.htm. Accessed 2011 August 22 ThigpenMCKebaabetswePMSmithDKSegolodiTMSoudFA 2011 Daily oral antiretroviral use for the prevention of HIV infection in heterosexually active young adults in Botswana: results from the TDF2 study. [Abstract WELBC01]. The 6th International AIDS Society Conference. Available: http://pag.ias2011.org/abstracts.aspx?aid=4631. Accessed 2011 August 22 BaetenJ 2011 Antiretroviral Pre-Exposure Prophylaxis for HIV-1 prevention among heterosexual African men and women: the Partners PrEP Study [Abstract MOAX0106]. The 6th International AIDS Society Conference. Available: http://pag.ias2011.org/flash.aspx?pid=886. Accessed 2011 August 22 SuntharasamaiPMartinMVanichseniSvan GriensvenFMockPA 2009 Factors associated with incarceration and incident HIV infection among injection drug users participating in an HIV vaccine trial in Bangkok, Thailand, 1999–2003. Addiction 104 235 242 19149819 VanichseniSTapperoJWPitisuttithumPKitayapornDMastroTD 2004 Recruitment, screening and characteristics of injection drug users participating in the AIDSVAX® B/E HIV vaccine trial, Bangkok, Thailand. AIDS 18 311 6 15075550 van GriensvenFKeawkungwalJTapperoJWSangkumUPitisuttithumP 2004 Lack of increased HIV risk behavior among injection drug users participating in the AIDSVAX B/E HIV vaccine trial in Bangkok, Thailand. AIDS 18 295 301 15075548 van GriensvenFPitisuttithumPVanichseniSWichienkuerPTapperoJW 2005 Trends in the injection of midazolam and other drugs and needle sharing among injection drug users enrolled in the AIDSVAX B/E HIV-1 vaccine trial in Bangkok, Thailand. Int J Drug Policy 16 171 5 MartinMVanichseniSSuntharasamaiPMockPAvan GriensvenF 2010 Drug use and the risk of HIV infection amongst injection drug users participating in an HIV vaccine trial in Bangkok, 1999–2003. Int J Drug Policy 21 296 301 20079620 Human Rights Watch 2004 Not Enough Graves: The War on Drugs, HIV/AIDS, and Violations of Human Rights. Human Rights Watch Report; 16:No. 8(C). Available: http://www.hrw.org/reports/2004/thailand0704/index.htm Accessed 2011 May 26 VongchakTKawichaiSShermanSCelentanoDDSirisanthanaT 2005 The influence of Thailand's 2003 ‘war on drugs’ policy on self-reported drug use among injection drug users in Chiang Mai, Thailand. International Journal of Drug Policy 16 115 121 MastroTDKitayapomDWenigerBGVanichseniSLaosunthomV 1994 Estimating the Number of HIV-Infected Injection Drug Users in Bangkok: A Capture-Recapture Method. Am J Public Health 84 1094 1099 8017531 WattanaWvan GriensvenFRhucharoenpornpanichOManopaiboonCThienkruaW 2007 Respondent-driven sampling to assess characteristics and estimate the number of injection drug users in Bangkok, Thailand. Drug and Alcohol Dependence 90 228 233 17507180 ChoopanyaKDes JarlaisDCVanichseniSKitayapornDMockPA 2002 Incarceration and risk for HIV infection among injection drug users in Bangkok. JAIDS 29 86 94 11782595 MirinSMMeyerREMendelsonJHEllingboeJ 1980 Opiate use and sexual function. Am J Psychiatry 137 909 15 6774622 DaniellHW 2002 Narcotic-induced hypogonadism during therapy for heroin addiction. J Addict Dis 21 47 53 12296501 KoningsEBantebyaGCaraëlMBagendaDMertensT 1995 Validating population surveys for the measurement of HIV/STD prevention indicators. AIDS 9 375 82 7794542 van GriensvenFNaoratSKilmarxPHJeeyapantSManopaiboonC 2006 Palmtop-assisted Self-Interviewing for the Collection of Sensitive Behavioral Data: Randomized Trial with Drug Use Urine Testing. Am J Epidemiol 163 271 8 16357109 Des JarlaisDCPaoneDMillikenJTurnerCFMillerH 1999 Audio-computer interviewing to measure risk behaviour for HIV among injecting drug users: a quasi-randomised trial. Lancet 353 1657 61 10335785 MetzgerDSKoblinBTurnerCNavalineHValentiF 2000 Randomized controlled trial of audio computer-assisted self-interviewing: utility and acceptability in longitudinal studies. Am J Epidemiol 152 99 106 10909945