Details:

Alternative Title:
RSC Adv
Publisher's site:
http://dx.doi.org/10.1039/C4RA07059C
Personal Author:
Hua, Xiude ; Liu, Xiaofeng ; Shi, Haiyan ; Wang, Yanru ; Kim, Hee Joo ; ... More +
Hua, Xiude ; Liu, Xiaofeng ; Shi, Haiyan ; Wang, Yanru ; Kim, Hee Joo ; Gee, Shirley J. ; Wang, Minghua ; Liu, Fengquan ; Hammock, Bruce D. ; Less -
Description:
An enzyme-linked immunosorbent assay (ELISA) was developed to detect organophosphorus pesticides using a phage-borne peptide that was isolated from a cyclic 8-residue peptide phage library. The IC50 values of the phage ELISA ranged from 1.4 to 92.1 μg L(-1) for eight organophosphorus pesticides (parathion-methyl, parathion, fenitrothion, cyanophos, EPN, paraoxon-methyl, paraoxon, fenitrooxon). The sensitivity was improved 120- and 2-fold compared to conventional homologous and heterologous ELISA, respectively. The selectivity of the phage ELISA was evaluated by measuring its cross-reactivity with 23 organophosphorus pesticides, among which eight were the main cross-reactants. The spike recoveries were between 66.1% and 101.6% for the detection of single pesticide residues of parathion-methyl, parathion and fenitrothion in Chinese cabbage, apple and greengrocery, and all of the coefficient of variation were less than or equal to 15.9%. Moreover, the phage ELISA results were validated by gas chromatography. The results indicate that isolating phage-borne peptides from phage display libraries is an alternative method for the development of a heterologous immunoassay and that the developed assay has a lower limit of detection than the chemically synthesized competitor assay.
Subject:
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Article
Enzyme-linked Immunosorbent Assay
Multi-analyte Assay
Organophosphorus Pesticides
Phage-borne Peptide

Source:
RSC Adv. 4(80):42445-42453. ;
Pubmed ID:
26290688
Pubmed Central ID:
PMC4539265
Document Type:
Journal Article ;
Funding:
P42 ES004699/ES/NIEHS NIH HHS/United States ; U50 OH007550/OH/NIOSH CDC HHS/United States ;
P42 ES004699/ES/NIEHS NIH HHS/United States ; U50 OH007550/OH/NIOSH CDC HHS/United States ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:25caa5bcdae85c4f534da5d90ba70f71e53ca10a50b5090a4ba095dac10ef67b
File Type:

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