i
Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide
-
1 1 2014
-
-
Source: RSC Adv. 4(80):42445-42453
[PDF-735.05 KB]
Details:
-
Alternative Title:RSC Adv
-
Personal Author:
-
Description:An enzyme-linked immunosorbent assay (ELISA) was developed to detect organophosphorus pesticides using a phage-borne peptide that was isolated from a cyclic 8-residue peptide phage library. The IC| values of the phage ELISA ranged from 1.4 to 92.1 μg L| for eight organophosphorus pesticides (parathion-methyl, parathion, fenitrothion, cyanophos, EPN, paraoxon-methyl, paraoxon, fenitrooxon). The sensitivity was improved 120- and 2-fold compared to conventional homologous and heterologous ELISA, respectively. The selectivity of the phage ELISA was evaluated by measuring its cross-reactivity with 23 organophosphorus pesticides, among which eight were the main cross-reactants. The spike recoveries were between 66.1% and 101.6% for the detection of single pesticide residues of parathion-methyl, parathion and fenitrothion in Chinese cabbage, apple and greengrocery, and all of the coefficient of variation were less than or equal to 15.9%. Moreover, the phage ELISA results were validated by gas chromatography. The results indicate that isolating phage-borne peptides from phage display libraries is an alternative method for the development of a heterologous immunoassay and that the developed assay has a lower limit of detection than the chemically synthesized competitor assay.
-
Subjects:
-
Source:
-
Pubmed ID:26290688
-
Pubmed Central ID:PMC4539265
-
Document Type:
-
Funding:
-
Collection(s):
-
Main Document Checksum:
-
Download URL:
-
File Type:
Supporting Files
-
jpeg 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg 
gif 
xml 
jpeg 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg 
gif 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg 
jpeg 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg 
gif 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg 
gif 
jpeg 
jpeg 
gif 
jpeg 
gif
