Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide

Details

• Alternative Title:
  RSC Adv
• Personal Author:
  Hua, Xiude ; Liu, Xiaofeng ; Shi, Haiyan ; Wang, Yanru ; Kim, Hee Joo ; Gee, Shirley J. ; Wang, Minghua ; Liu, Fengquan ; Hammock, Bruce D.
• Description:
  An enzyme-linked immunosorbent assay (ELISA) was developed to detect organophosphorus pesticides using a phage-borne peptide that was isolated from a cyclic 8-residue peptide phage library. The IC| values of the phage ELISA ranged from 1.4 to 92.1 μg L| for eight organophosphorus pesticides (parathion-methyl, parathion, fenitrothion, cyanophos, EPN, paraoxon-methyl, paraoxon, fenitrooxon). The sensitivity was improved 120- and 2-fold compared to conventional homologous and heterologous ELISA, respectively. The selectivity of the phage ELISA was evaluated by measuring its cross-reactivity with 23 organophosphorus pesticides, among which eight were the main cross-reactants. The spike recoveries were between 66.1% and 101.6% for the detection of single pesticide residues of parathion-methyl, parathion and fenitrothion in Chinese cabbage, apple and greengrocery, and all of the coefficient of variation were less than or equal to 15.9%. Moreover, the phage ELISA results were validated by gas chromatography. The results indicate that isolating phage-borne peptides from phage display libraries is an alternative method for the development of a heterologous immunoassay and that the developed assay has a lower limit of detection than the chemically synthesized competitor assay.
• Subjects:
  Article Enzyme-linked Immunosorbent Assay Multi-analyte Assay Organophosphorus Pesticides Phage-borne Peptide
• Source:
  RSC Adv. 4(80):42445-42453
• Pubmed ID:
  26290688
• Pubmed Central ID:
  PMC4539265
• Document Type:
  Journal Article
• Funding:
  P42 ES004699/ES/NIEHS NIH HHSUnited States/ ; U50 OH007550/OH/NIOSH CDC HHSUnited States/
• Volume:
  4
• Issue:
  80
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:25caa5bcdae85c4f534da5d90ba70f71e53ca10a50b5090a4ba095dac10ef67b
• Download URL:
  https://stacks.cdc.gov/view/cdc/42066/cdc_42066_DS1.pdf
• File Type:
  [PDF - 735.05 KB ]