Overv iew of Preventable Industrial Causes of Occupational Cancer Elizabeth Ward National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Department of Health and Human Services, Cincinnati, Ohio This paper summarizes what is known about preventable causes of occupational cancer, including single agents, complex mixtures, and broad occupational associations. Epidemiologic methods have been very successful in documenting cancer risks associated with single agents. Epidemiologic data are most conclusive when an exposure-response relationship can be demonstrated. Examples of agents for which epidemiologic studies provide evidence of an exposure-response relationship include benzene and (concurrent exposure to) ortho-toluidine and aniline. Vinyl chloride and bischloromethyl ether are examples of associations between single agents and rare histologic types of cancer. It is more difficult to conduct epidemiologic studies to identify cancer risks associated with complex mixtures. Studies of diesel exhaust and lung cancer and metal machining oils are cited as having employed advanced industrial hygiene and epidemiologic methods for studies of complex mixtures. Elevated cancer risks have also been identified in broad occupational groups, including painters and dry cleaners. Epidemiologic case-control studies are often used to detect such associations but are limited in their abilities to detect the causal agents. Major gaps exist in knowledge of occupational cancer risks .among women workers and workers of color. Because epidemiologic research measures illness and mortality that have already occurred, a positive study can be interpreted to represent a failure in prevention. The challenge we face in the next decade is to identify interventions earlier in the causal pathway (toxicologic testing, biomarkers of exposure or precancerous changes, institution of engineering and good industrial hygiene practices to reduce occupational exposure levels) so that occupational cancer can be prevented. - Environ Health Perspect 103(Suppl 8):197-203 (1995) Key words: carcinogen, standards, occupational health, epidemiology Introduction It is estimated that about 4% of all cancers are due to occupation (1), concentrated among lung and bladder cancer. A total of 10 to 20% of lung cancers (2) and 21 to 27% of bladder cancers (3,4) are estimated to be related to occupational exposure. This paper will summarize what is known about preventable causes of occupational cancer and highlight some critical gaps in knowledge. Knowledge of occupational cancer risks will be discussed in relation to This paper was presented at the President's Cancer Panel Conference on Avoidable Causes of Cancer held 7-8 April 1994 in Bethesda, Maryland. Manuscript received 9 March 1995; manuscript accepted 24 March 1995. Address correspondence to Elizabeth Ward, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Mail Stop R-13, 4676 Columbia Parkway, Cincinnati, OH 45226. Telephone: (513) 841-4203. Fax: (513) 841-4486. Abbreviations: BCME, bischloromethyl ether; Cl, confidence interval; U.S. EPA, U.S. Environmental Protection Agency; IARC, International Agency for Research on Cancer; NCI, National Cancer Institute; NCHS, National Center for Health Statistics; NTP, National Toxicology Program; NIOSH, National Institute for Occupational Safety and Health; OSHA, Occupational Safety and Health Administration; PCE, perchloroethylene; ppm, parts per million; PEL, per- missible exposure limit; REL, recommended expo- sure limit; RR, relative risk; SIR, standardized incidence ratio; SMR, standardized mortality ratio; pg/M3, micrograms per cubic meter. single agents, complex mixtures, and broad occupational categories. Sources of Data on Occupational Carcinogens The most comprehensive source of infor- mation about both occupational and non- occupational carcinogens is a series of monographs published by the International Agency for Research on Cancer (IARC) (5). These monographs are prepared with the help of international working groups of experts. IARC has published reviews on over 1000 substances. Each review contains a brief description of the chemical and physical properties of the agent, methods and volume of production, use patterns and occurrence, summaries of experimental carcinogenicity tests, a brief description of other relevant biological data (toxicity and genetic and related effects), summaries of case reports and epidemiologic studies of cancer in humans, and an evaluation of its carcinogenicity. IARC classifies agents (or exposure circumstances) according to their carcinogenicity, which ranges from car- cinogenic to humans (Group 1) to proba- bly not carcinogenic (Group 4). Among the 100 definite and probable carcinogens, approximately 40% involve primarily occupational exposures (6). Within the United States, the National Toxicology Program (NTP) publishes an annual report on carcinogens each year. This legislatively mandated document [Section 301(b) (4) of the Public Health Service Act] contains "a list of all substances which are known to be carcinogens or may reason- ably be anticipated to be carcinogens and to which a significant number of persons residing in the United States are exposed." It also contains information on the extent and nature of exposure and each standard established by a Federal agency with respect to the substance. The Seventh Annual Report on Carcinogens listed 24 substances known to be carcinogenic and approxi- mately 140 substances that are reasonably anticipated to be carcinogens (7). In the United States, the agencies man- dated to ensure the health and safety of the working population are the Occupational Safety and Health Administration (OSHA) within the Department of Labor, and the National Institute for Occupational Safety and Health (NIOSH) within the Centers for Disease Control and Prevention. OSHA, the agency charged with promul- gating and enforcing occupational health standards, has standards for 24 carcinogens (Table 1). These standards specify not only the permissible exposure limit (PEL) for the concentration of the substance in air but also other requirements for labeling, personal protective equipment, and med- ical screening. OSHA standards require Environmental Health Perspectives 197 E. WARD Table 1. Potential occupational carcinogens recognized by NIOSH. Acetaldehyde 2-Acetylaminogluorene (O)a Acrylamide Acrylamide Acrylonitrile (0) Aldrin 4-Aminodiphenyl (0) Amitrole Aniline and homologs o-Anisidine p-Anisidine Arsenic, inorganic (0) Arsine Asbestos (0) Asphalt fumes Benzene (0) Benzidine (0) Benzidine-based dyes Beryllium Butadiene tert-Butyl chromate; class, chromium, Cadmium dust and fume (0) Captafol Captan Carbon tetrachloride Chlordane Chlorinated camphene Chlorodiphenyl (42% chlorine); class, polychlorinated biphenyls Chlorodiphenyl (54% chlorine); class, polychlorinated biphenyls Chloroform Chloromethyl methyl ether (0) bis(Chloromethyl) ether (0) P-Chloroprene Chromium, hexavalent [Cr(VI)] Chromyl chloride; class, chromium, hexavalent Chrysene Coal tar pitch volatiles; class, coal tar products Coke oven emissions (0) DDT (dichlorodiphenyltrichloroethane) Di-2-ethylhexyl phthalate (DEHP) 2,4-Diaminoanisoleo O-Dianisidine-based dyes 1,2-Dibromo-3-chloropropane (0) Dichloroacetylene (DBCP) p- Dichlorobenzene 3,3' -Dichlorobenzidine (0) Dichloroethyl ether 1 ,3-Dichloropropene Dieldrin Diesel exhaust Diglycidyl ether (DGE); class, glycidyl ethers 4-Dimethylaminoazobenzene (0) Dimethyl carbamoyl chloride 1,1-Dimethylhydrazine; class, hydrazines Dimethyl sulfate Dinitrotoluene Dioxane Environmental tobacco smoke Epichlorohydrin Ethyl acrylate Ethylene dibromide Ethylene dichloride Ethylene oxide (0) Ethyleneimine (0) Ethylene thiourea Formaldehyde (0) Gallium arsenide 8O, Occupational Safety and Health Administration. Gasoline Heptachlor Hexachlorobutadiene Hexachloroethane Hexamethyl phosphoric triamide (HMPA) Hydrazine Kepone Methoxychlor Methyl bromide; class, monohalomethanes Methyl chloride Methylhydrazine Methyl iodide; class, monohalomethanes Methyl hydrazine; class, hydrazines 4,4'-Methylenebis(2-chloroaniline) (MBOCA) Methylene chloride 4,4'-Methylenedianiline (MDA) (0) a-Naphthylamine (0) 3-Naphthylamine (0) Nickel, metal, soluble, insoluble, and inorganic; class, nickel, inorganic Nickel carbonyl Nickel sulfide roasting 4-Nitrobiphenyl (0) p-Nitrochlorobenzene 2-Nitronaphthalene 2-Nitropropane N-Nitrosodimethylamine (0) Pentachloroethane; class, chloroethanes N-Phenyl-o-naphthylamine; class, 0-naphthylamine Phenyl glycidyl ether; class, glycidyl ethers Phenylhydrazine; class, hydrazines Propane sultone 3-Propiolactone (0) Propylene dichloride Propylene imine Propylene oxide Radon Rosin core solder, pyrolysis products (i.e., formaldehyde, acetaldehyde, malonaldehyde) Silica, crystalline cristobalite Silica, crystalline quartz Silica, crystalline tripoli Silica, crystalline tridymite Silica, fused Soapstone, total dust silicates Tremolite silicates 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) (dioxin) 1,1 ,2,2-Tetrachloroethane Tetrachloroethylene Titanium dioxide o-Tolidine-based dyes o-Tolidine Toluene diisocyanate (TDI) Toluenediamine (TDA) o-Toluidine p-Toluidine 1,1,2-Trichloroethane; class, chloroethanes Trichloroethylene 1 ,2,3-Trichloropropane Uranium soluble compounds Vinyl bromide; class, vinyl halides Vinyl chloride (0) Vinyl cyclohexene dioxide Vinylidene chloride (1,1-dichloroethylene); class, vinyl halides Welding fumes, total particulates Wood dust Zinc chromate; class, chromium companies to reduce exposures to specified levels. In addition to the comprehensive standards for the 24 carcinogens, OSHA may have PELs limiting occupational exposure to potential carcinogens based on other health effects. NIOSH, the agency charged with con- ducting occupational health research and issuing recommendations, has identified 131 substances as potential occupational carcinogens (Table 1). Many of these substances were identified by NIOSH as potential occupational carcinogens based on animal data alone. NIOSH recom- mends that exposure to these substances be reduced to the lowest feasible concentra- tion (8). While NIOSH-recommended exposure limits (RELs) are not legally enforceable, they serve as technology- forcing goals in reducing exposure. Occupational cancer epidemiology in the United States is conducted by a num- ber of Federal agencies, including NIOSH, the National Cancer Institute (NCI), and the National Institute for Environmental Health Sciences (NIEHS), as well as aca- demic institutions. Many of these agencies also have toxicology programs that evaluate chemicals for carcinogenicity. Although animal studies are critically important in the identification of potential occupational carcinogens and the interpretation of epi- demiologic study results, this paper will focus on data from epidemiologic studies. Single Agents as Preventable Causes of Occupational Cancer Identification of single agents as pre- ventable causes of occupational cancer is most frequently done by retrospective cohort studies. These studies require the identification of sizable populations exposed historically to the agent of interest in the absence of other potential carcino- gens. The populations are followed for can- cer mortality and/or incidence, allowing a 20- to 40-year latency period for cancers to develop. The confirmation of an agent as an occupational carcinogen is most persuasive when an exposure-response relationship is demonstrated. Exposure-response may be evaluated in the overall cohort, or in a nested case-control study when it is more efficient to characterize exposure in only a portion of the cohort. Data can be used in risk assessments to estimate the reduction in risk associated with a decrease in exposure. An example of a study demonstrating an exposure-response relationship is a Environmental Health Perspectives 198 PREVENTABLE CAUSES OF OCCUPATIONAL CANCER study of rubber hydrochloride workers potentially exposed to benzene at two plants (9,10). A total of 1165 white men with at least 1 ppm-day exposure to benzene between January 1, 1940, and December 31, 1965, were included in the study. Air monitoring data for benzene were available from the two plants as early as 1946. Based on these data, a matrix was developed linking each job title with an estimate of the time-weighted average ben- zene exposure. Linking this matrix with the individual job histories allowed calculation of the cumulative lifetime exposure (ppm- years) for each individual. Table 2 summa- rizes the results of the standardized mortality ratio (SMR) analysis, in which a pattern of higher risk with higher cumula- tive exposure was observed. This data set was used by OSHA in 1987 to demon- strate that the 1 ppm PEL for benzene would prevent a significant risk of material health impairment (11). In studies in which historical industrial hygiene data are unavailable, duration of exposure is sometimes used as a surrogate for dose. In a study of bladder cancer inci- dence among workers exposed concurrently to the aromatic amines ortho-toluidine and aniline in a rubber chemicals manufactur- ing plant, no historical industrial hygiene data were available (12). Investigators used duration of employment as a surrogate for dose, and found a striking relationship (Table 3). Strong associations also have been estab- lished for certain chemicals that cause rare types of cancer. For example, workers exposed to vinyl chloride have died from liver cancer at a rate seven times that expected; most of the deaths were due to angiosarcoma of the liver (13), a histologic type that accounts for under 1% of liver neo- plasms diagnosed in the United States (14). The carcinogenicity of bischloromethyl ether (BCME) was first recognized by the occurrence of three cases of small cell lung cancer among 45 workers from the same Table 2. Standardized mortality ratios (SMRs) for leukemia among workers exposed to benzene in a rubber hydrochloride plant. Benzene exposure, ppm/year 0.001-40 40-200 200-400 >400 Total Observed deaths 2 2 2 3 9 Expected deaths 1.83 0.62 0.17 0.04 2.66 SMR 1.09 3.22 11.86 66.37 3.37 Table 3. Standardized incidence ratios (SIRs) for bladder cancer among workers exposed to ortho-toluidine and aniline in a rubber chemicals manufacturing plant. Years in Number of Observed Expected department persons cases cases SIR < 5 584 0 0.75 - 5-9.99 51 1 0.11 8.8 > 10 73 6 0.22 27.2 Total 708 7 1.08 6.5 Adapted from Ward et al. (12). facility in a Philadelphia chemical plant (15). Summarizing the data to date, Steenland et al. (2) found that in a total of 3332 workers exposed to BCME studied worldwide, 98 lung cancer cases were observed and 24.1 were expected (SMR = 4.1). Studies have typically exhibited a marked dose response and a histologic specificity for small cell tumors (2). Because it is possible to develop evi- dence for a causal relationship between the specific agent and the cancer excess, single- agent carcinogens can be effectively con- trolled. Twenty-three of the 24 carcinogens regulated by OSHA, including benzene, asbestos, BCME, and vinyl chloride, are single-agent exposures. Complex Mixtures as Preventable Causes of Occupational Cancer Many occupational exposures involve complex mixtures rather than chemically specific substances. Diesel exhaust is an example of a complex mixture that is car- cinogenic in animals (16) and is consid- ered a probable human carcinogen by IARC and NIOSH. Levels of occupational exposure are difficult to quantify because until recently there was no air sampling method specific to diesel exhaust (17). In 1988, a review of the carcinogenicity of diesel exhaust concluded that the epidemi- ologic evidence was limited by the diffi- culty in defining and quantifying exposure, the relatively short time between initial exposures and analysis of risk in some stud- ies, and the need to control for cigarette smoking (18). Recent epidemiologic studies have improved on earlier research. Smoking data have been collected and an attempt has been made to measure diesel exposure cur- rently and then extrapolate back to histori- cal levels. For example, Steenland et al. (19) conducted a nested case-control study of lung cancer deaths within the Teamsters Union. Interviews with next of kin were conducted to determine smoking history; both Teamster Union records and next-of- kin interviews were used to determine work history. Odds ratios for jobs with diesel exposure were compared with those without. Long-term (>35 years) truck dri- vers of primarily diesel trucks had an odds ratio of 1.89 (95% confidence interval [CI] = 1.04-3.42); individuals whose main job was truck mechanic had an odds ratio of 1.69 (95% CI=0.92-3.09), which was not related to length of exposure. An industrial hygiene survey (17,20) used ele- mental carbon as a marker of diesel exhaust. This survey estimated that air con- centrations of elemental carbon in the work area of mechanics averaged 26.6 Pg/m3, while air concentrations in the cabs of long-haul trucks averaged 5.1 pg/m3. Background levels in residential areas average 1.1 pg/m3. There are approximately 1.5 million U.S. workers exposed to diesel exhaust, principally in the trucking and construction industry. Steenland et al. (2) estimated that approximately 800 of the 100,000 lung cancer cases diagnosed among U.S. males each year are attributable to occupational diesel exposure. Diesel exposures among long-haul truck drivers, the largest occupa- tional group exposed to diesel emissions, are being reduced by U.S. Environmental Protection Agency (U.S. EPA) regulations for allowable truck emissions. However, truck mechanics and railroad workers may continue to have substantial exposures. Another example of complex mixtures that have been recognized as potentially carcinogenic are cutting and lubricating oils. These products are thought to be responsible for the excesses of cancer of the scrotum, bladder, and digestive tract that have been observed among workers in the machining and metalworking occupations. In a recent example, a case-control study of occupational causes of bladder cancer found an elevated risk among drill press operatives (Table 4) that was related to length of exposure (3). In 1981, NIOSH estimated that there were 6 million workers in nonagricultural industries exposed to Table 4. Number cases and controls and relative risks (RR), according to duration of employment in the occu- pation of drill press operator, white males. Duration, Trend year Cases Controls RR test, p < 5 22 33 1.0 0.008 5-9 14 14 1.8 10+ 12 9 2.4 From Silverman et al. (3). Volume 103, Supplement 8, November 1995 Adapted from Rinsky et al. (10). 199 E. WARD mineral oils, 2 million to lubricating oils, 1 million to cutting oils, and 1 million to motor oils. The complexity and diversity of cutting oils, however, make it difficult to determine the actual carcinogenic exposure. A major epidemiologic study was con- ducted to examine the risks associated with the three major classes of machining fluids: straight oils (cutting oils), which are naph- thenic or paraffinic mineral oils; soluble oils, which contain emulsifying agents to suspend the oil drops in water; and syn- thetic oils, in which synthetic chemicals are substituted for oils (21). This cohort mor- tality study included more than 45,000 workers from three plants, almost 1 million person-years of follow-up, and over 10,000 deaths. An analysis of three subcohorts ever exposed to straight oils (n= 13,967), solu- ble oils (n=23,488) and synthetic fluids (n=8446) suggested associations of expo- sure to straight oil with rectal, laryngeal, and prostatic cancers. For each of these cancers, the SMR for those ever exposed to straight oils was elevated, there was some indication in the poisson modeling of a trend in risk with increasing duration of exposure, and the association was consis- tent across plants (Table 5) (22). No can- cer excesses were associated with soluble oil exposure. There was some evidence of an association between pancreatic cancer and exposure to synthetic fluids. In a separate set of analyses, investigators examined risk of several cancers in relation to quantitative estimates of cumulative exposure to specific types of machining fluids; some analyses were limited to grinding occupations. These analyses demonstrated an exposure- response relationship for exposure to straight oils and laryngeal cancer, for grind- ing operations and esophageal cancer, and for exposure to straight oils and rectal cancer. The rate ratio for rectal cancer increased in a monotonic fashion up to 2.9 (95% CI=1.3-5.0) in the highest exposure category. This study was unique both in terms of its massive size and extensive exposure char- acterization. The exposure characterization required that type of machining fluid used be assigned to each job-department-plant- calendar-year combination after review of plant purchasing and industrial hygiene records and interviews with plant personnel. Estimates of total machining fluid aerosol were based on current measurements of total aerosol collected by the investigators in assembly, machining, and grinding oper- ations in the three study plants and histori- cal air sampling data collected by the company as well as on extensive interviews with plant personnel (23). One of the rea- sons it was possible to conduct such a detailed exposure assessment was that the study was jointly sponsored by the General Motors Corporation and the United Autoworkers Union and therefore investi- gators had full access to company records, facilities, and knowledgeable personnel. In summary, studies of complex mix- tures may require more refined methods of exposure assessment than studies of single agents. In the diesel studies, a method of monitoring for a component of diesel exhaust not present in cigarette smoke, manufacturing emissions, or wood smoke was developed. In the machining fluids study, a detailed historical reconstruction was used to characterize exposure to different types of machining fluids. Development of better exposure assess- ment techniques is at the cutting edge of occupational epidemiology. Table 5. Relationship between duration of exposure to straight-oil and risk of rectal, laryngeal, and prostatic cancer. Exposure to Number of deaths Cause of death straight-oil, years due to cause of death Rate ratio 95% Cl Rectal cancer 16 1.0 >0-0.99 5 0.93 0.34-2.56 1.00-2.49 6 1.20 0.47-3.08 2.50-7.49 9 1.64 0.72-3.76 > 7.50 21 3.17 1.62-6.24 Laryngeal cancer 13 1.0 > 0-0.99 6 1.26 0.48-3.32 1.00-7.49 8 1.02 0.41-2.49 >7.50 11 2.02 0.86-4.75 Prostatic cancer - 64 1.0 >0-0.99 16 0.83 0.48-1.43 1.00-2.49 21 1.27 0.77-2.09 2.50-7.49 24 1.26 0.78-2.03 > 7.50 40 1.52 1.01-2.29 Cl, confidence interval. Adapted from Tolbert et al. (22). In addition to their utility in mixed- exposure situations, sophisticated exposure assessment techniques are needed to deter- mine a quantitative exposure-response relationship for single-agent studies. For example, Greife et al. (24) used 2350 air- sampling data points collected at 21 com- panies, to develop and validate a statistical model to estimate ethylene oxide exposure for a retrospective cohort mortality study in the medical supplies industry. Stewart et al. (unpublished data) developed a complex exposure assessment strategy for a cohort mortality study of workers exposed to acrylonitrile. The study included eight plants that had started production between 1952 and 1965, but seven of the eight started taking air samples only in the late 1970s, and even after that time there were no air sampling data available for most of the jobs. A variety of techniques were used, including a ratio method, which estimated exposure for unmonitored jobs by assuming that the ratios of similar jobs at different plants would be similar, and a homoge- neous exposure group method based on combining jobs with similar exposures. Occupational Groups at High Risk for Cancer In addition to identification of carcinogenic agents, associations between employment in particular occupations/industries and cancer have been identified by mortality surveillance projects and by case-control interview studies. A large mortality surveil- lance database has been created by NIOSH, NCI, and the National Center for Health Statistics (NCHS) by funding state health departments to code industry and occupation on death certificates. A total of 28 states are included for 2 years or more from 1979 to 1990, yielding a total of over 5,000,000 records (25). This data- base is available on public use tapes, not only to Federal agencies, but also to non- government researchers. In recent years, NIOSH researchers have utilized this data- base to examine cancer mortality among women (25) and occupational risk factors for breast cancer (26), and NCI researchers have used it to examine cancer mortality among farmers (27). In case-control studies, the occupa- tional histories of persons with cancer are compared with the occupational histories of persons without cancer. Usually the information on occupations and exposures is gathered by interview with the patient (or control) or the next of kin. The disad- vantages of these types of studies are that Environmental Health Perspectives 200 PREVENTABLE CAUSES OF OCCUPATIONAL CANCER the information on occupational exposures is usually less specific than in cohort stud- ies, and if the cases and controls are derived from the general population, it will be dif- ficult to detect associations with rare expo- sures or occupations. The advantages of case-control studies are that nonoccupa- tional risk factors such as smoking can be taken into account and entire lifetime occupational histories can be considered. Often the results of case-control studies are used to suggest cohort studies to per- form. Much of our knowledge about risks associated with particular occupations is derived from surveillance efforts and case-control interview studies. Painters have been shown to have increased risks of lung cancer and cancers of the esophagus, stomach, and bladder in many studies, while excesses of leukemia and cancers of the buccal cavity and larynx were observed less consistently (28). In 1989, IARC concluded that there is suffi- cient evidence for the carcinogenicity of occupational exposure as a painters. Thousands of chemical compounds are used in paint products as pigments, exten- ders, binders, solvents, and additives, some of which are recognized to be potential human carcinogens(28). Painters are employed in numerous industries, includ- ing heavy machine manufacturing, con- struction, automobile refinishing, and fine arts (28). In some of these applications, worker exposure may be controlled by standards related to specific components of the paint or solvents used (for example, chromium compounds, lead, toluene). Because there is little epidemiologic data to associate the elevated cancer risks in painters to specific exposures, it is not known whether standards put in place in the last 10 to 20 years will protect painters from the elevated cancer risks experienced by earlier cohorts. It is estimated that 500,000 individuals are employed as painters in the United States. Workers in the dry-cleaning industry are also known to have elevated cancer risks. Unlike many broad occupational associations, the risks of dry cleaning have been documented primarily by cohort studies. Studies have reported elevated rates for urinary tract (29-31), bladder (32,33), esophageal (29,33), pancreatic (32,34), colon (33), and lymphatic (29) cancers among dry-cleaning workers. In most of these studies, it could not be determined to which specific solvent or solvents each worker was exposed. Historically, solvents used in dry cleaning include carbon tetrachloride, petroleum solvents (Stoddard solvent), trichloroethylene, and per- chloroethylene (tetrachloroethylene, PCE). Approximately 500,000 people work in the dry-cleaning industry in the United States. Perchloroethylene is used by over 90% of all dry-cleaning plants (35). IARC has concluded that there is sufficient evi- dence for the carcinogenicity of per- chloroethylene in animals (5) based on positive bioassay results in two species of animals. Stoddard solvent, a petroleum- based mixture of alkane and aromatic hydrocarbons, is used by about 10% of all dry-cleaning plants. The OSHA PEL for both solvents is 100 ppm as an 8-hr time- weighted average exposure. NIOSH con- siders perchloroethylene a potential occupational carcinogen and recommends that exposure be reduced to the lowest fea- sible levels. The NIOSH-recommended exposure level (REL) for Stoddard solvent is 67 ppm. NIOSH investigators have identified an excess of esophageal cancer among a small cohort (n=625) of dry-cleaning workers exposed only to perchloroethylene (33). The SMR for esophageal cancer among workers with > 5 years of exposure and >20 years of latency was 7.17 (95% CI = 1.92-19.8). NIOSH and NCI researchers recently have conducted a nationwide search for epidemiologic study cohorts exposed only to perchloroethylene or Stoddard solvent. To summarize, occupational groups at high risk for cancer are readily identified by surveillance and case-control studies and may in addition be documented by cohort studies, particularly of unions representing occupational groups. It has been difficult to take preventive action because the specific causal agents are not known. Summary and Recommendations Although the cancer policies of OSHA and NIOSH do not require adequate epidemi- ologic evidence to regulate an occupational carcinogen, in the past human studies have been the most potent drivers for regulatory action. Increasingly, NIOSH and OSHA, as well as the U.S. EPA and other regulatory agencies, have emphasized the importance of animal studies in making regulatory decisions. Techniques of risk assessment have developed substantially over the past decade, and OSHA has used risk assess- ments based on animal data in developing several recent regulatory actions (butadi- ene, methylene chloride) (36,37). Some may question the use of animal studies in identifying potential human carcinogens (38), but a recent review article demon- strated that 25 to 30% of agents, sub- stances, or chemicals that have been causally or strongly associated with cancer in humans were first identified as being carcinogenic in experimental animals (39). Moreover, it is hoped that increased under- standing of the molecular mechanisms of cancer induction will allow the design of even more predictive animal models in the future (39). A related area of research that may reduce the gap between experimental mod- els and identification of cancer risks in human populations is molecular cancer epidemiology. This has been defined to include the estimation of internal dose through biological monitoring of the con- centration of chemicals or their metabolites in blood, urine, or other tissue; the estima- tion of biologically effective dose through measurement of the amount of carcinogen that has interacted with cellular macromole- cules including DNA and protein adducts; the detection of early biologic effects such as sister chromatid exchange, DNA hyper- ploidy, or oncogene activation; and the identification of genetic factors in suscepti- bility (40). Such measures of exposure, sus- ceptibility, and cellular damage may be helpful in correlating carcinogenic effects between species, investigating chemical- specific exposures and effects in mixed- exposure situations, and identifying biological changes in populations whose average or maximum latency is too short for cancer to be manifest. Eventually stud- ies may be designed to demonstrate a direct relationship between specific indicators of cellular damage and increased cancer risk, thus increasing the value of the indicator in detecting potential human carcinogens. Nonetheless, traditional epidemiologic studies will continue to be important in identifying and confirming occupational cancer risks. Traditional techniques of occupational epidemiology (retrospective cohort study with retrospective exposure assessment) are very effective in dealing with single-agent exposures. Creative epi- demiologic and industrial hygiene tech- niques have been utilized to investigate occupational risks associated with complex mixtures and to determine quantitative estimates of exposure-response. These efforts tend to be very costly because they require large populations and extensive exposure assessment activities. In contrast, although much is known about associations Volume 103, Supplement 8, November 1995 201 E. WARD between particular occupations and cancer risk, there has been less progress in identi- fying the specific causal agents. New methods are currently being developed to allow the identification of causal agents via case-control interview studies. Identifi- cation of the causal agents would facilitate prevention by allowing intervention to be targeted at the exposures of concern. The cancer risks associated with broad occupa- tional categories are important because they affect a large number of people. In addition to epidemiologic and industrial hygiene research to identify the causal agents responsible for elevated can- cer risks in certain occupations or associ- ated with exposure to complex mixtures, there is a great need for information about occupational cancer risks among women and minority workers (41,42). Studies of occupational cancer risks among female workers are particularly needed to examine risks of hormonally related cancer. Few studies of breast cancer, the most common incident cancer among U.S. women, have addressed occupational and environmental chemical exposures, and many cancer studies of industrial cohorts have excluded women (43). Although there is no reason to believe that minority and nonminority workers might have a differential responses to occupational carcinogens, there is sub- stantial evidence for differential assignment of minority and nonminority persons to higher exposure jobs within the same industry which results ultimately in differ- ential mortality patterns. For example, an observation of higher lung cancer risk among black steelworkers was one of the factors leading to the identification of coke oven emissions as potent human carcinogens (44). In addition to the importance of occu- pational cancer research to the prevention of cancer associated with occupation, occupational studies are important in the identification of carcinogens that may increase cancer in the general population. Many chemicals for which the cancer risks are best studied in the occupational environment are of concern because of their presence in consumer products or in the environment. 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