Details:

Alternative Title:
Food Environ Virol
Personal Author:
Kahler, Amy M. ; Cromeans, Theresa L. ; Metcalfe, Maureen G. ; Humphrey, Charles D. ; Hill, Vincent R.
Kahler, Amy M. ; Cromeans, Theresa L. ; Metcalfe, Maureen G. ; Humphrey, Charles D. ; Hill, Vincent R. Less -
Description:
It is generally accepted that viral particles in source water are likely to be found as aggregates attached to other particles. For this reason, it is important to investigate the disinfection efficacy of chlorine on aggregated viruses. A method to produce adenovirus particle aggregation was developed for this study. Negative stain electron microscopy was used to measure aggregation before and after addition of virus particles to surface water at different pH and specific conductance levels. The impact of aggregation on the efficacy of chlorine disinfection was also examined. Disinfection experiments with human adenovirus 2 (HAdV2) in source water were conducted using 0.2 mg/L free chlorine at 5 °C. Aggregation of HAdV2 in source water (≥3 aggregated particles) remained higher at higher specific conductance and pH levels. However, aggregation was highly variable, with the percentage of particles present in aggregates ranging from 43 to 71 %. Upon addition into source water, the aggregation percentage dropped dramatically. On average, chlorination CT values (chlorine concentration in mg/L × time in min) for 3-log10 inactivation of aggregated HAdV2 were up to three times higher than those for dispersed HAdV2, indicating that aggregation reduced the disinfection rate. This information can be used by water utilities and regulators to guide decision making regarding disinfection of viruses in water.
Subjects:
[+]
Adenovirus Aggregation Article Chlorine Disinfection Water
Source:
Food Environ Virol. 8(2):148-155.
Pubmed ID:
26910058
Pubmed Central ID:
PMC4864101
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:6a7698beb397ee4eba5e67607eef9ed2d4bf395f5c1c63749fa8ea73b1971f91
Download URL:
https://stacks.cdc.gov/view/cdc/39452/cdc_39452_DS1.pdf
File Type:

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