Augmentation of Pulmonary Reactions to Quartz Inhalation by Trace Amounts of Iron-containing Particles Vincent Castranova,l Val Vallyathan,' Dawn M. Ramsey,2 Jeffrey L. McLaurin,2 Donna Pack,1 Steven Leonard,1 Mark W. Barger,1 Jane Y.C. Ma,1 Nar S. Dalai,3 and Alex Teass2 1Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia; 2Division of Biomedical and Behavioral Science, National Institute for Occupational Safety and Health, Cincinnati, Ohio; 3Department of Chemistry, Florida State University, Tallahassee, Florida Fracturing quartz produces silica-based radicals on the fracture planes and generates hydroxyl radicals (OH) in aqueous media. 'OH production has been shown to be directly associated with quartz-induced cell damage and phagocyte activation in vitro. This 'OH production in vitro is inhibited by desferrioxamine mesylate, an Fe chelator, indicating involvement of a Fenton-like reaction. Our objective was to determine if Fe contamination increased the ability of inhaled quartz to cause inflammation and lung injury. Male Fischer 344 rats were exposed 5 hr/day for 10 days to filtered air, 20 mg/m3 freshly milled quartz (57 ppm Fe), or 20 mg/m3 freshly milled quartz contaminated with Fe (430 ppm Fe). High Fe contamination of quartz produced approximately 57% more reactive species in water than quartz with low Fe contamination. Compared to inhalation of quartz with low Fe contamination, high Fe contamination of quartz resulted in increases in the following responses: leukocyte recruitment (537%), lavageable red blood cells (157%), macrophage production of oxygen radicals measured by electron spin resonance or chemiluminescence (32 or 90%, respectively), nitric oxide production by macrophages (71 %), and lipid peroxidation of lung tissue (38%). These results suggest that inhalation of freshly fractured quartz contaminated with trace levels of Fe may be more pathogenic than inhalation of quartz alone. - Environ Health Perspect 105(Suppl 5):1319-1324 (1997) Key words: Fenton reaction, silicosis, reactive oxygen species, inhalation, iron, nitric oxide, pulmonary inflammation, silica Introduction Occupational exposure to crystalline silica can result in the development of pulmonary fibrosis. Silicosis may develop rapidly (1-3 years) in the acute form of the disease. Acute silicosis is characterized by dyspnea, fatigue, cough, weight loss, alveolar This paper is based on a presentation at The Sixth International Meeting on the Toxicology of Natural and Man-Made Fibrous and Non-Fibrous Particles held 15-18 September 1996 in Lake Placid, New York. Manuscript received at EHP 26 March 1997; accepted 23 April 1997. Address correspondence to Dr. V. Castranova, PPRB/HELD/NIOSH, 1095 Willowdale Road (M/S 2015), Morgantown, WV 26505. Telephone: (304) 285-6056. Fax: (304) 285-5938. E-mail: vicl cdc.gov Abbreviations used: AM, alveolar macrophage(s); CL, chemiluminescence; ESR, electron spin reso- nance; 'OH, hydroxyl radical(s); 02-, superoxide anion; PBN, N-tert-butyl-phenylnitrone; PHPA, 4-hydroxyphenylacetic acid; RBC, red blood cell(s); 'Si, silicon radical; Si-O, silicon oxyl radical. lipoproteinosis, and diffuse fibrosis (1). In contrast, chronic silicosis develops over a period of 20 to 40 years. It is characterized by nodular lesions containing collagen in a spiral arrangement and may progress to massive fibrosis in which restrictive lung impairment is evident (2,3). Cellular injury and tissue damage are believed to be important steps in the devel- opment of silicosis (4). This damage may result directly from the cytotoxicity of quartz or indirectly from oxidant species produced by silica-activated pulmonary phagocytes (5). Such oxidants may indude superoxide anion (02-), hydrogen peroxide (H202), hydroxyl radical ('OH), nitric oxide (NO'), and peroxynitrite (6,7). Acute silicosis is associated with the generation of freshly fractured quartz dust in occupations such as sandblasting, rock drilling, tunneling, and silica milling. When crystalline quartz is fractured, silicon (CSi) and silicon oxyl radicals (Si-'O) respectively are formed on the fracture planes (8). On contact with aqueous media, these surface radicals generate 'OH (9). The Fe chelator desferrioxamine sig- nificantly reduces this 'OH production by freshly fractured quartz in water (10). This suggests that trace contamination of quartz with ferrous Fe can enhance 'OH genera- tion via a Fenton-like reaction as follows: Si-'O+ H20 ->SiOH + 'OH Si-'O+ 'OH -*SiOOH SiOOH + H20 -SiOH + H202 Fe2+ + H202 -*Fe3+ +'OH+OH- In vitro, freshly fractured quartz causes greater peroxidation of membrane lipids, greater membrane leakage, and a greater decrease in cell viability than aged quartz. Freshly fractured quartz is also a more potent stimulant of reactive species pro- duction by alveolar macrophages (AM) in vitro (9,11). In comparison to aged quartz, inhalation of freshly milled quartz results in a significantly enhanced cytotoxicity (lipid peroxidation of lung tissue and lavage levels of red blood cells [RBC], pro- tein, and lysosomal enzymes) and elevated inflammation (lavageable neutrophils, gen- eration of oxidant species from AM, and histological scoring of lung infiltrates) (12,13). Evidence suggests that a direct relationship exists between 'OH generation and cytotoxicity, as the production of both 'OH- and quartz-induced biological reac- tions decrease in a similar fashion with time after fracturing (9,11). Because Fe enhances 'OH generation by freshly fractured quartz and a relation- ship exists between 'OH production and the cytotoxic potency of quartz, the ques- tion was whether trace amounts of Fe would increase the pulmonary responses to inhaled quartz. To resolve this question, we conducted an inhalation exposure in which rats were exposed to filtered air, freshly milled quartz with low Fe contami- nation, and freshly milled quartz contain- ing 7.5 times more Fe contamination. Low and high Fe contamination of quartz was achieved by altering the length of the poly- ethylene delivery tube of the stainless steel screw feeder. The following pulmonary responses to quartz inhalation (20 mg/m3, 5 hr/day, 10 days) were monitored 1 day Environmental Health Perspectives * Vol 105, Supplement 5 * September 1997 1319 CASTRANOVA ET AL. postexposure: lavageable RBC, AM, and leukocytes; production of reactive species from AM; and lipid peroxidation of lung tissue. Methods Experimental Design Male Fischer 344 rats (7 weeks of age) were divided into three groups of 20 rats each (filtered air controls, freshly milled quartz with low Fe contamination, and freshly milled quartz with high Fe contamination) and housed in two 10 m3 Hinners-type walk-in chambers (quartz exposed) or a 5 m3 Hinners-type chamber (air control). Freshly milled quartz was generated in an air jet mill (Trost Gem-T Research Model Type 1047 [Garlock Inc., Newtown, Pa] fitted with a polyurethane liner; P-jet pres- sure of 70 psi and an 0-jet pressure of 100 psi). Low and high Fe contamination of quartz, i.e., quartz with low Fe contamina- tion and quartz with high Fe contamina- tion were achieved by adjusting the length of the screw feeder delivery tube (6.35 vs 15 cm to produce low vs high Fe contami- nation, respectively). Rats were exposed 5 hr/day for 10 days and were sacrificed 1 day postexposure for determination of biological responses. Details of the quartz dose, particle diameter, and trace metal contamination for the exposure groups are given in Table 1. Note that both quartz inhalation groups were exposed to particles of the same size (2.0-2.1 pm) and at the same dose (21-19 mg/m3). The major dif- ference between the two quartz aerosols was their trace metal content (57 ppm Fe for low Fe contamination and 430 ppm for high Fe contamination). Generation of Freshly Milled Silica Freshly fractured a-quartz was prepared from a stock IOTA lot 11383 quartz sam- ple obtained from Unimin Corporation (Spruce Pine, NC). This stock quartz had a mass mean diameter of 191 pim and was semiconductor grade in purity, i.e., greater than 99% pure crystalline silica (SiO2) as determined by X-ray diffraction analysis under scanning electron microscopy and optical microscopic examination under polarized light. Freshly fractured a-quartz was prepared as follows: stock of quartz dust was supplied by an AccuRate Series 100 dry material feeder (AccuRate, Whitewater, WI) with a 6.4-mm diameter stainless steel helix and a polyethylene delivery tube to a Trost Gem-T Research Model Type 1047 jet mill (Garlock, Newtown, PA) fitted with a polyurethane liner and stainless steel jets and operated with dry, charcoal-scrubbed compressed air at a P-jet pressure of 480 kPa (70 psi) and an 0-jet pressure of 690 kPa (100 psi). The difference in trace metal content of the two quartz aerosols is due to stain- less steel contamination from the dry material feeder. The longer the stock quartz remained in contact with the stain- less steel helix, the higher the resulting contamination. Shortening the length of the polyethylene delivery tube shortened the contact time, thus lowering the stain- less steel content of dust going to the jet mill. Inhalation Exposure The air supply to the animal chambers was rough filtered; chilled or heated to 16?C; reheated to 23?C (feedback control from the chambers); passed through a high effi- ciency particle filter, a charcoal bed, and a medium efficiency filter; and rehumidified with a steam humidifier. Air flow through the animal chambers was 12 air changes/hr and pressure in the chambers was held to 0.25 cm of water less than in the labora- tory. The aerosol of freshly milled a-quartz was passed directly from the air jet mill via 16 and 19 mm (inside diameter) tubing Table 1. Characteristics of exposure aerosols. Group SI dose,a mg/m3 Particle diameter,b Pm Iron,c ppm Air control 0 Si, low Fe contamination 21 ? 0.1 2.0 (1.9) 57 ?33 Si, high Fe contamination 19 ? 0.1 2.1 (2.0) 430 ? 73 "Silica concentration within the exposure chambers was determined gravimetrically from filter samples. Mean ? SD (n= 54). bMass median aerodynamic diameter was determined using a nine-stage Andersen cascade impactor ACFM particle-sizing sampler (Andersen Samplers, Atlanta, GA). Median value (geometric SD) (n=3). cTrace Fe contamination was determined by proton-induced X-ray emission spectroscopy performed by Element Analysis Corporation (Lexington, KY). Mean ? SD (n= 10). Other trace contaminants were Si with low Fe contamination:16 ppm chromium, 0.6 ppm copper, 1.2 ppm manganese, 7.2 ppm nickel, 1.3 ppm zinc, and 11 mg/g carbon. Carbon was measured by Galbraith Laboratories (Knoxville, TN) (n=5). Si with high Fe contamination:107 ppm chromium, 2.0 ppm copper, 11 ppm manganese, 47 ppm nickel, 0.7 ppm zinc, and 7.9 mg/g carbon. through a BGI Aero-2 cyclone (BGI Inc., Waltham, MA) to the mixing chamber in the air duct 46 cm upstream of the exposure chamber. To aid mixing of the two streams, the juncture was 3 cm down- stream of an orifice plate and 20 cm upstream of a circular baffle centered in the duct. The static charge on the dust was neutralized by a Static Control Services Model PFC-20 pulse flow controller (Static Control Service, Palm Springs, CA) with a Model AN-6 ionizing air nozzle located about 5 cm upstream of the mixing chamber. Exposures were conducted dur- ing the animals' dark cycles (i.e., during their active periods). Preliminary experi- ments with a fog generator revealed that baffling resulted in single-pass flow of air through the animal cages within the exposure chambers. Temperature (1 8.5-24.2?C), humidity (37-68%), and ammonia levels in the chambers were monitored continuously. Filter samples of the chamber atmospheres were obtained continuously to determine dust concentration by gravimetric analysis. Additional filter samples were obtained to monitor trace contaminants, dust activity (i.e., H202production), surface radicals by electron spin resonance (ESR) spec- troscopy, and particle size by scanning electron microscopy. The mass median aerodynamic diameter of the quartz parti- cles was determined using a nine-stage Andersen cascade impactor ACFM parti- cle-sizing sampler (Andersen Samplers, Atlanta, GA). Measurement of Surfac Rmadicals Surface reactivity of freshly milled and aged quartz was determined by monitoring surface free radicals using ESR spec- troscopy. ESR spectra were obtained with a Bruker Model ER300 spectrometer (Braker Instrument Corp., Billerica, MD) interfaced with an ASPECT 3000 micro- computer (Braker Instrument Corp.). The filter samples of dust were rolled, placed into a quartz ESR sample tube, and inserted into the center of the ESR cavity. All measurements were conducted at room temperature. The following settings were used: microwave power= 13 dB, microwave frequency= 9.53 GHz, modu- lation frequency= 100 kHz, modulation amplitude = 1 G, and receiver phase= 0 or 180. Silicon-based radical sites were char- acterized by ESR spectra centered around g= 2.0015. Surface activity was deter- mined from peak height and expressed as signal height per milligram of quartz. Environmental Health Perspectives * Vol 105, Supplement 5 * September 1997 1 320 TRACE IRON-ENHANCED RESPONSE TO QUARTZ INHALATION Measurement of Silica Reactive Products Generation of reactive products by freshly fractured quartz in aqueous media was determined by monitoring H202 produc- tion fluorometrically. The peroxidase-cat- alyzed oxidation of 4-hydroxyphenylacetic acid (PHPA) by H202 yields a strongly flu- orescent dimer of PHPA (14). In our appli- cation, the quartz-laden filter was placed into 10 ml of buffer solution (5 mM KNaHPO4; pH 7) and sonicated for 1 min. The filter was then rinsed with deionized water to remove residual dust. The suspen- sion was agitated at 25?C for 30 min to allow efficient transfer of H202 into the solution. The supernatant was made 0.024 mM in PHPA and 8.9 activity U/ml in per- oxidase and stored in the dark for 30 min to allow for reaction. The pH was then adjusted to greater than 10 and analysis was carried out with a flow injection sys- tem and a fluorescence detector (excitation 320 nm, emission 420 nm). The system was calibrated with standard solutions of H202 and measurements were corrected for sample blank and volume dilution. Detemination of Lavagable Cells Animals were anesthetized and lungs were lavaged 10 times with 8-ml aliquots of Ca2+, Mg2+-free phosphate-buffered medium (145 mM NaCl, 5 mM KCl, 1.9 mM NaH2PO4, 9.35 mM Na2HPO4, and 5.5 mM glucose; pH 7.4). Lavage fluid was centrifuged, and cells were collected, washed, and resuspended in HEPES- buffered medium (145 mM NaCl, 5 mM KCl, 10 mM HEPES, 5.5 mM glucose, and 1 mM CaCI2; pH 7.4). Cell counts were obtained using an electronic cell counter. Cell differentials were determined using a cell sizing attachment and charac- teristic cell volumes to count RBC, leuko- cytes (lymphocytes and granulocytes), and AM (15). Measurement of Alveolar Macpha Activity Chemiluminescence (CL) generated from pulmonary phagocytes was measured at 37?C in the presence of 8 pg% luminol using a Berthold LB953 luminometer (Wallac, Gaithersburg, MD). AM (7.5 x 105 cells) were suspended in 0.75 ml of HEPES-buffered medium and preincu- bated at 37?C for 10 min prior to being placed into the luminometer. Zymosan- stimulated CL (counts per minute with zymosan minus counts per minute at rest) was measured in the presence of 2 mg/ml unopsonized zymosan added just before measuring CL. Note that granulocytes do not respond to unopsonized zymosan (15). NO synthase-dependent CL (counts per minute with zymosan minus counts per minute with zymosan plus L-NAME [Nw-nitro-L-arginine methyl ester]) was determined as the amount of zymosan- stimulated CL that was inhibitable by 1 mM L-NAME added during the preincubation period. Oxygen radical generation from alveolar phagocytes was measured with an electron spin resonance spectrometer using a radical spin trap. AM (5 x 105 cells/ml) obtained from the three groups of animals were incubated with 0.1 M N-tert-butyl-phenyl- nitrone (PBN) for 1 hr at 37?C in an oscil- lating water bath. Control experiments were carried out without cells and without PBN. The reaction was terminated by the addition of 5 ml chloroform:methanol (2:1) and total lipids were extracted. The chloroform was evaporated in a boiling water bath and the lipid was reconstituted in 0.5 ml chloroform and transferred to nuclear magnetic resonance quartz tubes. On the basis of a method of Vallyathan and colleagues (16), ESR measurements of the chloroform extracts were made within 1 hr using a Varian E-109 ESR spectrophotometer (Varian Associates, Palo Alto, CA) operating at X-band (9.7 GHz) at the following spectrometer set- tings: receiver gain, 3.2 x 105; microwave power, 50 mW; modulation amplitude, 2 G; scan time, 100 sec; time constant, 1 sec; magnetic field, 3417? 50 G. ESR spectra were recorded and integrated for three scans as a measure of reactive oxygen species generated from phagocytes. Scaling and analysis of the spectra were made using the EPR (U.S. EPR, Clarksville, MD) DAP 2.0 program. Measurement of the Lung Lipid Peroxidation Lipid peroxidation of lung tissue from the three groups of animals was monitored by measuring thiobarbituric acid reactive sub- stances generated during incubation of lung slices for 1 hr in a buffered medium without any other stimulation, according to the method of Hunter and co-workers (17). Thin lung tissue slices (300-450 mg) were incubated in phosphate-buffered medium at pH 7.4 for 1 hr at 37?C in a shaking water bath. The reaction was ter- minated by the addition of 0.3 ml 5 N HCI and 0.625 ml 40% trichloroacetic acid. After mixing, 0.625 ml thiobarbituric acid was added to the reaction mixture, mixed, and heated in a water bath at 95?C for 20 min. The thiobarbituric acid- reactive substances developed a color that was measured after cooling at 540 nm and compared with malondialdehyde reaction with thiobarbituric acid. Malondialdehyde production was calculated from a standard graph that was made using the same reagents and known concentrations of mal- ondialdehyde. Control experiments were carried out without lung tissues, with inac- tivated lung tissue, and in the presence of an antioxidant (butyl hydroxy toluene) to inhibit lipid peroxidation. Statstical Ana is Data are means ? standard errors of 4 to 8 values determined from separate rats. Data were analyzed by analysis of variance to determine significance at p < 0.05. Results Freshly fracturing quartz in a jet mill breaks Si-O bonds and generates 'Si and Si-O' radicals on the fracture planes. As shown in Figure 1, trace contamination with stainless steel particles has no effect on the genera- tion of these surface radicals. Indeed, there is no difference in the intensity of the ESR signal produced by quartz with low Fe con- tamination compared with that produced by quartz with high Fe contamination after jet milling (intensity ratio of 1 and 0.93 for Si [low Fe contamination] and Si [high Fe contamination], respectively). In contrast, once placed in aqueous media, quartz with high Fe contamination produces more reactive species than quartz 0.8 - 'a a 0.6- cc cn ue X 0.4 co X 0.2- = 0.0 - Figure 1. 'Si and Si-0' radicals on the fracture planes of quartz after milling. Surface radicals were measured by ESR. Quartz with low Fe contamination and quartz with high Fe contamination contain 57 and 430 ppm Fe particles, respectively. Data represent signal heights (means ? SEM from four chamber samples). Environmental Health Perspectives * Vol 105, Supplement 5 * September 1997 1321 CASTRANOVA ET AL. with low Fe contamination (Figure 2). These data suggest that trace contamination of quartz with Fe particles (430 ppm) is sufficient to allow Fenton-like reactions to occur and result in enhanced generation of reactive species by freshly fractured quartz. Bronchoalveolar lavage indicates that inhalation of quartz with high Fe contami- nation causes significantly more damage to the alveolar air-blood barrier than an equal exposure to quartz with low Fe con- tamination, as measured by the lavage yield of RBC (Figure 3). Freshly milled silica contaminated with high Fe is also more inflammatory than milled silica with low Fe contamination, as judged by the infiltration of leukocytes (lymphocytes and granulocytes) into the airspaces of the lung (Figure 3). The activity of AM harvested from quartz-exposed rats was determined by monitoring: zymosan-induced chemilumi- nescence, NO-dependent chemilumines- cence, and the production of oxygen radicals using ESR and a spintrap. Inhalation of quartz contaminated with stainless steel Fe particles, i.e., quartz with high Fe contamination, significantly increases zymosan-stimulated chemilumi- nescence from AM above that seen after exposure to quartz with low Fe contamina- tion (Figure 4). Similar Fe enhancement is observed when NO-dependent (L-NAME- inhibitable) macrophage chemilumines- cence is monitored in response to zymosan (Figure 5) and when measuring the gen- eration of oxygen radicals by alveolar phagocytes (Figure 6). The enhanced production of reactive species in response to inhalation of quartz with high Fe contamination compared to quartz with low Fe contamination results in a greater level of lipid peroxidation in lung tissue exposed to high Fe-contaminated quartz (Figure 7). This increased lung dam- age may explain the breakdown of the alve- olar air-blood barrier suggested in Figure 3, i.e, the increase in lavageable RBC. 0 250- *0 a 200 CE ,~~~ ._ _ =a - X c ~~~~150- C.) 10 0 , E _ UC~~~~T 50- co 0 0- z, high Fe .. N- 250- * C 0 T - -a 4? 150 = C.0 0 WCD C.0 0 X A. AA=.- 0 Figure 2. Production of reactive species by freshly fractured quartz in aqueous medium. Quartz with low Fe contamination and quartz with high Fe contamina- tion contain 57 and 430 ppm Fe particles, respectively. Data represent means ? SEM from eight chamber sam- ples. *Significant increase above quartz with low Fe contamination (p<0.05). Figure 4. Zymosan-stimulated chemiluminescence from rat alveolar macrophages harvested by bron- choalveolar lavage 1 day after quartz exposure (20 mg/m3, 5 hr/day for 10 days). Quartz with low Fe cont- amination and quartz with high Fe contamination con- tain 57 and 430 ppm Fe particles, respectively. Data are means ?SEM from four rats. *Significant increase above quartz with low Fe contamination (p< 0.05). Figure 6. Generation of oxygen radicals produced by rat alveolar phagocytes harvested by bronchoalveolar lavage 1 day after quartz exposure (20 mg/m3, 5 hr/day for 10 days). Oxygen radicals are measured by ESR using a spin trap. Quartz with low Fe contamination and quartz with high Fe contamination contain 57 and 430 ppm Fe particles, respectively. Data are means ? SEM from four rats. - AM _ RBC _ Leukocytes n - -I | Filtered air (luhrtz, low Fe Quartz, high Fe Figure 3. AM, RBC, and leukocytes (lymphocytes and granulocytes) harvested by bronchoalveolar lavage 1 day after quartz exposure (20 mg/m3 , 5 hr/day for 10 days). Quartz with low Fe contamination and quartz with high Fe contamination contain 57 and 430 ppm Fe particles, respectively. Data are means ? SEM from eight rats. *Significant increase above quartz with low Fe contamination (p<0.05). Figure 5. NO-dependent chemiluminescence from rat AM harvested by bronchoalveolar lavage 1 day after quartz exposure (20 mg/m3, 5 hr/day for 10 days). Quartz with low Fe contamination and quartz with high Fe contamination contain 57 and 430 ppm Fe particles, respectively. Data are means ? SEM from four rats. *Significant increase above quartz with low Fe contam- ination (p<0.05). Figure 7. Lipid peroxidation of lung tissue measured as malondialehyde production 1 day after quartz expo- sure (20 mg/m3, 5 hr/day for 10 days). Quartz with low Fe contamination and quartz with high Fe contamina- tion contain 57 and 430 ppm Fe particles, respectively. Data are means ? SEM from four rat lungs. *Significant increase above quartz with low Fe contamination) (p<0.05). Environmental Health Perspectives * Vol 105, Supplement 5 * September 1997 0.15- a Cu ._ Ca 0. <, '4 o.lo- C D a E *o 0 , E 0.05- -o S0 0. Ca cd T 0.00 'L- T 900- 800- o, 700 C 600 0 m 0 500- - 400 CD * 200- 1 00- 250- * Cu co 200- 0 - '- 150- _ uOq -? 100 _) 0 ' 0 Cc Cu" CD 50- z I T 250 - 200- C 0 co -0+ 150- .0 0. "C.) 50 - 0- u o " .1 1 322 TRACE IRON-ENHANCED RESPONSE TO QUARTZ INHALATION Discussion The results of this investigation indicate that pulmonary responses 1 day after inhalation exposure to a-quartz are rather small when the quartz used is of semicon- ductor purity and efforts are employed to minimize trace metal contamination by the dust generation system. The simple increase in the length of the delivery tube of the screw feeder is sufficient to increase the Fe contamination of silica from 57 to 430 ppm. Such trace Fe contamination increases the generation of reactive species by silica in aqueous media by 52%. Trace Fe contami- nation also results in augmentation of pul- monary responses to quartz inhalation (Table 2). That is, in comparison to quartz with low Fe contamination, quartz with high Fe contamination increases leukocyte recruitment by 537%, lavagable RBC by 157%, phagocyte production of oxygen rad- icals by 32%, AM zymosan-stimulated chemiluminescence by 90%, NO-depen- dent chemiluminescence from AM by 71%, and lung lipid peroxidation by 38%. Ghio et al. (18) have proposed that silica-induced lung disease is mediated by surface coordination with Fe and the sub- sequent generation of 'OH via the Fenton reaction. Indeed, silica-Fe complexation has been demonstrated in rat lungs after intratracheal instillation of silica (19). In vitro treatment of silica with desferrioxam- ine mesylate (an Fe chelator) decreases sil- ica-induced oxidation of lung surfactant and the silica-stimulated respiratory burst of AM (20,21). Similarly, desferrioxamine decreases the ability of freshly fractured quartz to generate 'OH in aqueous media (10). Such pulmonary production of 'OH has been demonstrated in quartz-exposed rats and has been associated with lung inflammation (22). The results of the cur- rent investigation support the hypothesis Table 2. Increased activity of quartz with high Fe con- tamination. Percent increase quartz, high Fe: Parameter quartz, low Fe Quartz reactive species 52 Lavage RBC 157 Lavage leukocytes 537 AM zymo-stim CL 90 AM NO'-dependent CL 71 AM oxygen radicals 32 Lung lipid peroxidation 38 Zymo-stim, zymosan-stimulated. that trace Fe contamination can augment the generation of oxidants by quartz and enhance its inflammatory and cytotoxic potency in the lung. In addition, our results extend the hypothesis by demon- strating that this augmentation is possible with particulate Fe contamination as might be experienced in occupational settings such as rock drilling or sandblasting. In contrast to the above hypothesis that Fe contamination increases the cytox- icity of quartz, Nolan et al. (23) have reported that Fe chloride solutions decrease the in vitro hemolytic potential of quartz by neutralizing the negative surface charge of quartz. Similarly, Fe chloride solutions can decrease quartz-induced hydrogen peroxide secretion from AM in vitro (24). Particulate Fe is also protective against quartz-induced pulmonary inflam- mation (neutrophil recruitment) after intratracheal instillation of carbonyl Fe plus quartz (24). This apparent conflict is resolved by an analysis of the Fe dose required to neutralize the negative surface charge of quartz and result in significant detoxification. In solution, as much as 1 M Fe chloride is required to eliminate the negative surface charge of quartz, whereas as a particulate, Fe must be 12-fold in excess of quartz for depression of toxicity to become apparent (24). In contrast, quartz-Fe complexes in the lung are asso- ciated with only trace levels of Fe (19). Furthermore, the current investigation clearly demonstrates that trace contamina- tion of quartz with Fe particles (430 ppm) enhances pulmonary inflammation and lung damage. In this study, the reactivity of the freshly fractured quartz was associated with the level of Fe contamination. However, it must be noted that the two quartz aerosols investigated in this study really differed in their contamination by stainless steel partides. Thus, as demonstrated in Table 1, amounts of numerous trace metals (chromium, copper, manganese and nickel) as well as Fe were elevated in the more toxic quartz sample. Emphasis has been placed on Fe because of the extensive literature associating Fe with the generation of *OH via the Fenton reaction. However, evidence exists that chromium (Cr3+) can also gener- ate 'OH in the presence of H202(25). In addition, nickel (Ni2+) has been reported to generate 'OH from hydroperoxides but only in the presence of oligopeptides such as glutathione (26). 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