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The stability of hexacosanoyl lysophosphatidylcholine in dried-blood spot quality control materials for X-linked adrenoleukodystrophy newborn screening
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Oct 12 2014
Source: Clin Biochem. 48(1-2):8-10. -
Alternative Title:Clin Biochem
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Description:Objectives
Newborn screening for X-linked adrenoleukodystrophy utilizes tandem mass spectrometry to analyze dried-blood spot specimens. Quality control materials (dried-blood spots enriched with hexacosanoyl lysophosphatidylcholine) were prepared and stored at different temperatures for up to 518 days to evaluate the stability of this biomarker for X-linked adrenoleukodystrophy.
Design and methods
Dried-blood spot storage included desiccant (45, 171, and 518 days) or omitted desiccant (53 days at >90% relative humidity). Specimens were stored for 171 and 518 days at −20 °C, 4 °C, ambient temperature, and 37 °C. Each weekday for 45 days, a bag of specimens stored at 4 °C was warmed to ambient temperature and one specimen was removed for storage at −80 °C. Specimens were analyzed by high-performance liquid-chromatography electrospray ionization tandem mass spectrometry and data was plotted as concentration (micromoles per liter) vs. time. Linear regression provided slope and y-intercept values for each storage condition.
Results
Small slope values (0.01 or less) and y-intercept values close to the enrichment indicated less than 11% loss of hexacosanoyl lysophosphatidylcholine under all storage conditions tested.
Conclusions
Quality control materials for X-linked adrenoleukodystrophy are stable for at least 1 year when stored with desiccant.
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Pubmed ID:25307302
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Pubmed Central ID:PMC4835029
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