Characterization of 137 genomic DNA reference materials for 28 pharmacogenetic genes: A GeT-RM collaborative project

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• Alternative Title:
  J Mol Diagn
• Personal Author:
  Pratt, Victoria M. ; Everts, Robin E. ; Aggarwal, Praful ; Beyer, Brittany N. ; Broeckel, Ulrich ; Epstein-Baak, Ruth ; Hujsak, Paul ; Kornreich, Ruth ; Liao, Jun ; Lorier, Rachel ; Scott, Stuart A. ; Smith, Chingying Huang ; Toji, Lorraine H. ; Turner, Amy ; Kalman, Lisa V.
• Description:
  Pharmacogenetic testing is increasingly available from clinical laboratories. However, only a limited number of quality control and other reference materials are currently available to support clinical testing. To address this need, the Centers for Disease Control and Prevention-based Genetic Testing Reference Material Coordination Program, in collaboration with members of the pharmacogenetic testing community and the Coriell Cell Repositories, has characterized 137 genomic DNA samples for 28 genes commonly genotyped by pharmacogenetic testing assays (CYP1A1, CYP1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A4, CYP3A5, CYP4F2, DPYD, GSTM1, GSTP1, GSTT1, NAT1, NAT2, SLC15A2, SLC22A2, SLCO1B1, SLCO2B1, TPMT, UGT1A1, UGT2B7, UGT2B15, UGT2B17, and VKORC1). One hundred thirty-seven Coriell cell lines were selected based on ethnic diversity and partial genotype characterization from earlier testing. DNA samples were coded and distributed to volunteer testing laboratories for targeted genotyping using a number of commercially available and laboratory developed tests. Through consensus verification, we confirmed the presence of at least 108 variant pharmacogenetic alleles. These samples are also being characterized by other pharmacogenetic assays, including next-generation sequencing, which will be reported separately. Genotyping results were consistent among laboratories, with most differences in allele assignments attributed to assay design and variability in reported allele nomenclature, particularly for CYP2D6, UGT1A1, and VKORC1. These publicly available samples will help ensure the accuracy of pharmacogenetic testing.
• Subjects:
  Article Base Sequence Carrier Proteins Cell Line Cytochrome P-450 Enzyme System Drug-Related Side Effects And Adverse Reactions Genetic Testing Genotype Genotyping Techniques Glucuronosyltransferase Glutathione Transferase High-Throughput Nucleotide Sequencing Humans Pharmacogenetics Polymorphism, Single Nucleotide Sequence Analysis, DNA

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• Source:
  J Mol Diagn. 18(1):109-123.
• Pubmed ID:
  26621101
• Pubmed Central ID:
  PMC4695224
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States ; K23 GM104401/GM/NIGMS NIH HHS/United States ; U01 HG007762/HG/NHGRI NIH HHS/United States ; U01HG007762/HG/NHGRI NIH HHS/United States
• Place as Subject:
  United States
• Volume:
  18
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:dcbd32db62c2724917cdb72322bb91fcb8d59f91f7282c5f7e1d89cf7fc3a9f7
• Download URL:
  https://stacks.cdc.gov/view/cdc/37129/cdc_37129_DS1.pdf
• File Type:
  [PDF - 327.49 KB ]