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Efficient and quantitative high-throughput transfer RNA sequencing
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9 2015
Source: Nat Methods. 12(9):835-837 -
Alternative Title:Nat Methods
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Description:Despite its biological importance, tRNA has not been adequately sequenced by standard methods because of its abundant post-transcriptional modifications and stable structure, which interfere with cDNA synthesis. We achieved efficient and quantitative tRNA sequencing in HEK293T cells by using engineered demethylases to remove base methylations and a highly processive thermostable group II intron reverse transcriptase to overcome these obstacles. Our method, DM-tRNA-seq, should be applicable to investigations of tRNA in all organisms.
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Pubmed ID:26214130
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Pubmed Central ID:PMC4624326
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