Effect of mucosal cytokine administration on selective expansion of vaginal dendritic cells to support nanoparticle transport
Published Date:Jun 27 2015
Source:Am J Reprod Immunol. 74(4):333-344.
Granulocyte-Macrophage Colony-Stimulating Factor
Mice, Inbred C57BL
Pubmed Central ID:PMC4599983
Funding:1DP2HD075703/DP/NCCDPHP CDC HHS/United States
DP2 HD075703/HD/NICHD NIH HHS/United States
T32AI07140/AI/NIAID NIH HHS/United States
The capacity of antigen-carrying vaccine nanoparticles administered vaginally to stimulate local immune responses may be limited by the relatively low numbers of antigen-presenting cells (APCs) in the genital mucosa. Because inflammation is associated with increased susceptibility to sexually transmitted infections, we sought to increase APC numbers without causing inflammation.
Method of Study
In this study, we evaluated intravaginal delivery of chemokines, growth factors, or synthetic adjuvants to expand APCs in reproductive tissues.
We found that granulocyte macrophage-colony stimulating factor (GM-CSF) stimulated expansion of CD11b+ dendritic cells within 24 h of intravaginal administration, with no effect on Langerhans cells or macrophages. Expansion of the CD11b+ DC population was not associated with increased inflammatory cytokine production, and these cells retained phagocytic function.
Our data suggest that non-inflammatory expansion of mucosal APCs by intravaginal GM-CSF could be used as an adjuvanting strategy to potentiate the genital immune response to nanoparticulate mucosal vaccines.
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