02555625985N Engl J MedN. Engl. J. Med.The New England journal of medicine0028-47931533-440624131194461600010.1056/NEJMc1305535HHSPA727976ArticleSpread of Pacific Northwest Vibrio parahaemolyticus StrainMartinez-UrtazaJaimePh.D.European Center for Disease Prevention and Control, Stockholm, SwedenBaker-AustinCraigPh.D.craig.baker-austin@cefas.co.ukCentre for Environment, Fisheries and Aquaculture Science, Weymouth, United KingdomJonesJessica L.Ph.D.Food and Drug Administration, Dauphin Island, ALNewtonAnna E.M.P.H.Centers for Disease Control and Prevention, Atlanta, GAGonzalez-AvilesGladys D.M.Sc.Centers for Disease Control and Prevention, Atlanta, GADePaolaAngeloPh.D.Food and Drug Administration, Dauphin Island, AL610201517102013231020153691615731574To the Editor

Globally, Vibrio parahaemolyticus is a leading cause of seafood-associated gastroenteritis. Historically, the V. parahaemolyticus serotypes O4:K12 and O4:KUT, which have been shown to be more virulent than other pathogenic V. parahaemolyticus strains, have been unique to the Pacific Northwest region.1 These strains, which were first identified in the Pacific Northwest in 1988, caused large U.S. outbreaks in 1997 and 2004.2 The outbreaks were linked to consumption of Pacific shellfish, but these strains have not been linked to illness or isolated outside the Pacific Northwest until recently.

During the summer of 2012, outbreaks of V. parahaemolyticus infection caused by these serotypes occurred on the Atlantic coasts of the United States and Spain. The U.S. Centers for Disease Control and Prevention received reports of 28 cases of infection from 9 states, and regional health authorities in Galicia in northwest Spain received reports of 51 cases, of which 9 were subsequently confirmed by laboratory tests.

The U.S. outbreak was linked to consumption of shellfish harvested from Oyster Bay Harbor, New York, between April and August 2012. The Spanish outbreak occurred in August 2012 on a cruise ship in Galicia and was epidemiologically linked to cooked seafood cooled with ice produced from untreated local seawater.

Clinical isolates obtained from U.S. and Spanish patients were highly similar to Pacific Northwest strains on serologic tests, polymerase-chain-reaction assays for virulence factors, pulsed-field gel electrophoresis, and multilocus sequence typing (Table 1). The specificity of these subtyping methods used in combination indicates commonality among outbreak strains. The initial arrival and overall environmental distribution of, as well as the mechanism for the introduction of, the V. parahaemolyticus strains to the Atlantic coasts of the United States and Spain are unknown. However, importation and storage of live contaminated bivalve shellfish in local waters, ballast water movement,3 and long-distance oceanic transportation of strains into new regions4 are possible mechanisms. Both New York and Galicia have previously had outbreaks of V. parahaemolyticus infection caused by other strains, and both cities are near major ports. Both the U.S. and Spanish outbreaks corresponded temporally and spatially with higher-than-normal surface seawater temperatures, which have been associated with an increase in both the environmental prevalence of V. parahaemolyticus and illnesses.5

These strains were associated with illnesses on the U.S. Atlantic coast again this year; however, it is uncertain whether these strains will continue to persist or spread to other regions. Europe currently lacks systematic surveillance for noncholera vibrio species; this limits the identification of new strains and estimates of the burden of illness. We advocate continued and improved national and international collaboration and data sharing to help recognize and respond to future outbreaks of V. parahaemolyticus infection.

Disclosure forms provided by the authors are available with the full text of this letter at NEJM.org.

TurnerJWParanjpyeRNLandisEDPopulation structure of clinical and environmental Vibrio parahaemolyticus from the Pacific Northwest coast of the United StatesPLoS One201382e5572623409028McLaughlinJBDePaolaABoppCAOutbreak of Vibrio parahaemolyticus gastroenteritis associated with Alaskan oystersN Engl J Med20053531463147016207848DePaolaACapersGMMotesMLIsolation of Latin American epidemic strain of Vibrio cholerae O1 from US Gulf CoastLancet19923396241347133Martinez-UrtazaJHuapayaBGavilanRGEmergence of Asiatic Vibrio diseases in South America in phase with El NiñoEpidemiology20081982983718854707Martinez-UrtazaJBowersJCTrinanesJDePaolaAClimate anomalies and the increasing risk of Vibrio parahaemolyticus and Vibrio vulnificus illnessesFood Res Int20104317801790

Vibrio parahaemolyticus Strains from Outbreaks in 2012 in the United States and Spain and Closely Matching Pacific Northwest Isolates.*

StrainYear of Isolationand CharacterizationSourceLocationSerotype
F11–3A1988EnvironmentalWashington StateO4:K12
480571990ClinicalWashington StateO4:K12
102961997ClinicalWashington StateO4:K12
029–1(b)1997EnvironmentalOregonO4:K12
103291998ClinicalWashington StateO4:K12
CDC_2012V-11092012ClinicalOyster Bay Harbor, New YorkO4:K12
CDC_2012V-11082012ClinicalOyster Bay Harbor, New YorkO4:K12
CDC_M12–108 G2012ClinicalOyster Bay Harbor, New YorkO4:KUT
CDC_2012V-11312012ClinicalCaliforniaO4:KUT
CDC_2012V-11322012ClinicalCaliforniaO4:K12
CDC_2012V-11342012ClinicalOyster Bay Harbor, New YorkO4:KUT
G352012ClinicalSpainO4:K12
G362012ClinicalSpainO4:K12
G372012ClinicalSpainO4:K12

All isolates, which were positive for thermostable direct hemolysin and thermostable direct-related hemolysin, were multilocus sequence type 36.