Determination of a Viral Load Threshold To Distinguish Symptomatic versus Asymptomatic Rotavirus Infection in a High-Disease-Burden African Population

Bennett, A.; Bar-Zeev, N.; Jere, K. C.; Tate, J. E.; Parashar, U. D.; Nakagomi, O.; Heyderman, R. S.; French, N.; Iturriza-Gomara, M.; Cunliffe, N. A.

doi:10.1128/JCM.00875-15

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Determination of a Viral Load Threshold To Distinguish Symptomatic versus Asymptomatic Rotavirus Infection in a High-Disease-Burden African Population

Published Date:

Apr 08 2015

Publisher's site:

http://dx.doi.org/10.1128/JCM.00875-15

Source:

J Clin Microbiol. 2015; 53(6):1951-1954.

[PDF-331.15 KB]

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Alternative Title:

J Clin Microbiol

Personal Author:

Bennett, A. ; Bar-Zeev, N. ; Jere, K. C. ; Tate, J. E. ; Parashar, U. D. ; ... More ▼

Description:

We evaluated quantitative real-time PCR to establish the diagnosis of rotavirus gastroenteritis in a high-disease-burden population in Malawi using enzyme immunoassay as the gold standard diagnostic test. In 146 children with acute gastroenteritis and 65 asymptomatic children, we defined a cutoff point in the threshold cycle value (26.7) that predicts rotavirus-attributable gastroenteritis in this population. These data will inform the evaluation of direct and indirect rotavirus vaccine effects in Africa.

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Document Type:

Journal Article

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CDC Public Access

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Determination of a Viral Load Threshold To Distinguish Symptomatic versus Asymptomatic Rotavirus Infection in a High-Disease-Burden African Population

Determination of a Viral Load Threshold To Distinguish Symptomatic versus Asymptomatic Rotavirus Infection in a High-Disease-Burden African Population

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