Environmental Health Perspectives Vol. 49, pp. 223-231, 1983 In Vivo Metabolism of 3,2'*Dimethyl*4 Aminobiphenyl (DMAB) Bearing on Its Organotropism in the Syrian Golden Hamster and the F344 Rat. by Miriam Nussbaum,* Emerich S. Fiala,*t Bharati Kulkarni,* Karam EIBayoumy* and John H. Weisburger* The in vivo metabolism of tritiated DMAB was examined in male Syrian golden hamsters, which are susceptible to both urinary bladder and intestinal carcinogenesis by this agent and in male F344 rats in which intestinal tumors represent the main lesions. Evidence was obtained for the pres- ence of the N-hydroxy-N-glucuronide of DMAB as a major metabolite in hamster urine and bile and in rat bile but not urine. The routes of excretion of this metabolite, which may represent a transport form of the ultimate carcinogen, correlate well with the main tumor sites in the two species. Other metabolites partially identified were the sulfates and glucuronides of C-hydroxylated DMAB and C- hydroxylated-N-acetyl DMAB. Introduction During comparative carcinogenicity studies with various derivatives of 4-aminobiphenyl, Walpole et al. (1-3) observed that the introduction of a methyl group ortho to the amine function (3-methyl-4-amino- biphenyl) resulted in increased carcinogenicity to- wards the intestinal tract of rats. On the other hand, methyl substitution meta to the amine (2- methyl-4-aminobiphenyl) decreased the carcinogenic- ity and changed the organotropism, with the ap- pearance of liver rather than intestinal tumors. The introduction of a second methyl group at the 2' posi- tion to give 3,2'-dimethyl-4-aminobiphenyl (DMAB), enhanced the carcinogenicity toward the intestinal tract even further; moreover significant carcinoge- nicity appeared toward the ear duct, the salivary gland and other organs. Interestingly a methoxy group ortho to the amine produced a carcinogen with organotropism toward the urinary bladder. These relationships are summarized in Table 1. To *Naylor Dana Institute for Disease Prevention, American Health Foundation, Valhalla, NY 10595. tAuthor to whom correspondence should be addressed. explain the carcinogenicity of 4-aminobiphenyl, 3- methyl-4-aminobiphenyl and DMAB toward the small and large intestines, Walpole speculated (1) that the effective carcinogen of the amines was a metabolite excreted in the bile. Because of the great interest in animal models for colorectal cancer which would accurately reflect the disease in man, DMAB was utilized by Spjut et al. (4-6), and So and Wynder (7) and others (8) to study the induction and development of colon cancer in ro- dents. In experiments designed to test Walpole's suggestion that metabolites of DMAB acted topi- cally on the intestine, Navarrette-Reyna and Spjut (9) performed colostomies 4 cm above the rectum in rats. Following the SC administration of DMAB, they found that tumors were found exclusively in the intestine proximal to the colostomy. Other ex- periments by Cleveland et al. (10), involving the SC administration of DMAB to rats with surgically de- functionalized colon segments, similarly indicated that tumors appeared only in those segments which were in actual contact with the fecal stream. These experiments provided strong evidence that the in- duction of tumors in the intestine was related to the transport of some form of the carcinogen via the NUSSBA UM ET AL. Table 1. Incidence of tumors in albino Wistar rats.i' Incidence of tumors Total dose Intestine Bladder Mammary Salivary Ear duct L iver SC, G/KG NH2 3.6-5.8 7/14 - 3/12(0) - - H3 ( NH;! 1.2 12/23 - - - 3 23 - ICH3 H3 H 2 2.8-3.5 21/23 1/23 1,12(0) 5 23 4 23 0 CH3 NJ 2 2.4 - - - - 3 23 H3 Q Q N H Z 44 - 9/10 - "Data from Walpole et al. (1-3). bile and intestinal contents rather than by the blood stream. In the experiments of So and Wynder (7), an in- teresting species difference in the response to DMAB was observed: whereas in the rat the pre- dominant sites of tumors were the small and large intestine, in the hamster DMAB caused a high inci- dence of tumors in the urinary bladder. This species difference was confirmed by later work (11, 12) and is illustrated in Table 2. To attempt to elucidate the mechanisms of the contrasting organotropisms of DMAB in the rat and hamster, we began studies on the in vivo (13) as Table 2. Organotropism of 3.2-dimethyl-4-aminobiphenyl in male Syrian golden hamsters and F344 rats.a Incidence,% Organ Hamsters (25) Rats (26) Stomach, squamous papillomas 48 0 Urinary bladder, carcinoma 64 0 Small intestine, adenocarcinoma 28 35 Colon, adenocarcinoma 24 31 Ear duct 0 8 Lung 0 19 Skin 0 54 Subcutaneous tissue 0 23 Prostate, carcinoma in situ 0 19 aData of Fiala et al. (12). well as the in vitro metabolism of the carcinogen. The results of our continuing in vivo studies are presented here. Materials and Methods Animals Male outbred Syrian golden hamsters and male F344 rats were obtained from Charles River Breed- ing Laboratories. The animals were maintained on NIH-07 diet and water ad libitum. The approximate mean weight of the hamsters used for these studies was 175 g; that of the rats was 225 g. Chemicals DMAB HCl was obtained from Ash Stevens, De- troit, Mich. The material was custom tritiated by New England Nuclear Corp. For purification, 25-30 mCi of the tritiated DMAB was mixed with 0.5-1.0 g of unlabeled DMAB HCl in a small volume of ether. After conversion of the HC1 salt to the free base by addition of an excess of saturated NaHCO3 solution with stirring, the mixture was extracted several times with ether. The ether extracts were combined and passed through ClinElut columns (Fisher Scien- tific) to remove water and concentrated by rotary evaporation to yield a viscous oil. This was diluted with an equal volume of ether and applied to an 224 IN VI VO METABOLISM OF DMAB E.M. Merck size C Lobar silica gel column equili- brated with n-hexane- ethanol, 96:4 (v/v). The column was eluted with the same solvent at a rate of 0.5 mL/min. After determination of radioactivity, frac- tions containing DMAB were identified by serially spotting aliquots onto a TLC plate and spraying with Ehrlich reagent (14). The fractions showing a yellow color on the plate with the reagent were pooled and concentrated to yield a clear viscous oil which turned into a white solid on storage at 4?C. The radiochemical purity was > 97% by TLC on sil- ica gel [benzene- CHCl3- ethyl acetate- methanol, 70:15:15:3 (v/v); Rf = 0.63] and by HPLC [two Waters /Bondapak C18 columns in series eluted with methanol-H20, 75:25, (v/v); elution volume, 11.5 ml]. For use as chromatographic standards, the N-hy- droxy and C-nitroso derivatives were synthesized from DMAB by the method described by Hecht et al. (15). For enzymatic hydrolyses, E. coli P-glucuronidase (Sigma type IX) and Helix pomatia aryl sulfatase (Sigma type H-1) were used. All other chemicals and solvents were reagent or HPLC grade and distilled deionized H20 was used in all phases of these ex- periments. Collection of Bile Rats or hamsters were anesthetized and the com- mon bile duct was cannulated with polyethylene tubing, size No. 1. In early experiments, ether was used as the anesthetic. In later experiments, ether was used in combination with halothane for more uniform anesthesia. No differences in biliary excre- tion which could be ascribed to the method of anes- thesia were noted. In the rat, the cannula emerged from the animal by way of the posterior aspect of the hind leg. In the hamster, the gall bladder was li- gated before cannulation of the bile duct and the cannula was channeled subcutaneously to the inter- scapular area and exteriorized at that point. Triti- ated DMAB was suspended in corn oil and was in- jected SC at a dose level of 75 mg/kg immediately after the cannulation of the bile duct. Approxi- mately a 20 ,.Ci dose was given to each animal. The animals were placed in plastic restrainers and bile was collected in test tubes cooled to 0-40C. Collection of Urine Nonoperated animals were treated with tritiated DMAB as above and placed in stainless steel metab- olism cages with provisions for the separation of urine and feces. Urine was collected at 0-40C in con- tainers to which 0.5 mL of 0.5M triethylamine-CO2 buffer, pH 8.0, was added to maintain alkaline pH. All animals had free access to food and water dur- ing either bile or urine collection. Sephadex LH-20 Chromatography For most of the experiments described here, a wet-packed 1.5 x 110 cm column of Sephadex LH- 20 (25-100 ,um) equilibrated with methanol- H20- 0.5M triethylamine-CO2 buffer, pH 8.0, 100:100:2 (vlv), was used. The column was eluted at a rate of 12 mLlhr, and 6 mL fractions were collected. The in- clusion of the triethylamine buffer in the eluant served not only to maintain moderately alkaline pH, but also appeared to increase resolution signifi- cantly, an effect which might be due in part to ion- pairing with the acid conjugated metabolites. The separation of conjugated urinary o-toluidine metabo- lites by this technique was reported by us earlier (16). Thin-Layer Chromatography Silica gel G or GF plates, 250 ,m, were obtained from Analtech, Newark, Del. For the separation of glucuronic and sulfuric acid conjugates, the plates were developed with n-butanol-acetic acid- water, 3:1:1 (vlv). We refer to this as system A. For the separation of DMAB, 3,2'-dimethyl-4-nitrosobiphenyl, and various aglycones from enzymatic hydrolyses,. the plates were developed with system B: benzene- chloroform-ethyl acetate-methanol, 70:15:15:3 (v/v). High Pressure Liquid Chromatography A Waters Model M600A HPLC pump with a U6K injector was used in conjunction with a Schoef- feVKratos SF 770 variable wavelength monitor. Mass Spectrometry Mass spectra were obtained with a Hewlett-Pack- ard 5980A mass spectrometer, equipped with a Model 5933A data system, at 70 eV with a source temperature of 175-180?C. Total Urinary and Biliary Radioactivity A comparison of the total amount of radioactivity excreted in a 24-hr period after dosing with 75 mg/ kg of tritiated DMAB by the rat and hamster via the bile and urine is shown in Figure 1. While the rat excreted approximately 6% of the dose in the urine and 12% in the bile, the hamster excreted ap- proximately 17% in the urine and 5% in the bile. In those cases where collections were extended to 48 hrs, the rats excreted an additional 5.6 + 2.6% of the dose in the urine, 10.9 ? 3.10% in the bile, and the hamsters excreted an additional 3.9 ? 1.6% in the urine and 3.1% (only one hamster with a bile fis- tula survived 48 hr) in the bile. 225 NUSSBA UM ET AL. w& 151 CO) 0 a use. o HAMSTER 10 (4) 5 RAT (6) ~~~~~~~HAMSTER FIGURE 1. Excretion of radioactivity in rat and hamster urine and bile in 24 hr following an SC injection of tritiated DMAB (75 mg/kg). Parentheses denote number of animals used. Separation of DMAB Metabolites Submission of the 0-24 hr samples of bile and urine of the two species to Sephadex LH-20 chroma- tography yielded the metabolite profiles shown in Figures 2 and 3. While only three major metabo- lites, designated a, P and y were resolved from rat bile, at least seven metabolite peaks were detected in rat urine (Fig. 2). Peaks P and y were present in both bile and urine, peak a, a major component was present only in the bile while peaks 6 and E and two other late-eluting minor peaks were present in significant amounts only in the urine. In the hamster (Fig. 3), peaks corresponding to the elution volumes of rat metabolites a, P and y were present in both bile and urine, and peak a was the major metabolite in both fluids. Sephadex LH-20 peaks a and P from rat bile, ham- ster bile or rat and hamster urine were positive for glucuronic acid by the naphthoresorcinol reaction, as described by Mead et al. (17), indicating glucu- ronic acid conjugates. TLC of combined concentrated fractions compris- ing a peak a on silica gel plates eluted with system A showed one radioactive peak near the solvent front which developed yellow color immediately with Ehrlich reagent but was negative to naphtho- resorcinol. However, the latter reagent showed a distinct blue zone on the plate at R_ = 0.42 which was devoid of radioactivity and which corresponded to free glucuronic acid. This suggested that a glucu- ronic acid conjugate in peak a was hydrolyzed by the acid TLC solvent. To determine whether or not this was the case, a portion of the combined, concentrated a peak from co o U- n 0 z 0 U- C-, cc RAT URINE RAT BILE 60 120 180 240 300 360 ml FIGURE 2. Sephadex LH-20 profiles of DMAB metabolites in rat urine and rat bile collected for 24 hr following tritiated DMAB administration. rat bile was adjusted to pH 5.0 with sodium acetate buffer and incubated at 370C. Aliquots were taken periodically and submitted to reverse-phase HPLC using methanol-water, 75:25 (vlv), as eluant. With increasing time of incubation, radioactivity in the most polar peak gradually decreased with corre- sponding increases in radioactivity in peaks at elu- tion volumes of 8.5 and 16.5 mL (Fig. 4). The elution volumes of these peaks corresponded exactly to those of DMAB and 3,2'-dimethyl-4-nitrosobiphenyl standards, respectively. At short times of incuba- tion, e.g., 30-60 minutes, a radioactive peak ap- peared at an elution volume of 7.0 mL which corre- sponded to N-hydroxy-DMAB. This peak disap- peared after longer times of incubation, presumably due to further oxidation to the nitroso form. After 3 hr of incubation, the mixture was extracted with ether and the ether extract submitted to TLC using 226 IN VIVO METABOLISM OF DMAB HAMSTER URINE 30 20 10 I 020- 2 0 I A. HAMSTER BILE 10 GLUCURONIDE CS) O MIN n N-HYDROXY DMAB 30 MIN \ DMAB NITROSO DMB 90MIN *4 8 12 16 60 120 180 240 300 ml FIGURE 3. Sephadex LH-20 profiles of DMAB metabolites in hamster urine and hamster bile collected for 24 hr follow- ing tritiated DMAB administration. solvent system B. Two major radioactive ultravio- let-absorbing bands were observed at Rf = 0.63, corresponding to DMAB, and at Rf = 0.79, corre- sponding to 3,2'-dimethyl-4-nitrosobiphenyl. These bands were eluted and submitted to mass spectrom- etry (Fig. 5) whereby their identity was confirmed. The time course of hydrolysis of the a peak from rat bile at pH values of 5.0, 6.2 and 7.8 is shown in Figure 6. At pH 7.8 very little hydrolysis occurred over the 3-hr period, but hydrolysis was rapid at pH 6.2 and more so at pH 5.0. At the latter pH approxi- mately 80% of the a peak was hydrolyzed within 3 hr. Two alternatives concerning the origin of the DMAB and the nitroso derivative were considered. It was possible that the a peak obtained by Sepha- dex LH-20 chromatography was not homogeneous and contained the N-glucuronide of DMAB as well ELUTION VOLUME, ml FIGURE 4. HPLC analyses of rat bile a peak incubated for varying times at pH 5 and 370C. as the N-glucuronide of N-hydroxy-DMAB. Such N- glucuronides are well known to be hydrolyzed under mildly acidic conditions (18-21). In the pres- ence of oxygen, the N-hydroxy-DMAB aglycone would be rapidly oxidized to the nitroso form. On the other hand, it was possible that the a peak con- sisted entirely of the N-hydroxy-N-glucuronide of DMAB. After hydrolysis, the N-hydroxy aglycone could conceivably disproportionate to DMAB and the nitroso product. To determine which was the case, the a peaks ob- tained from rat bile and hamster bile and urine were submitted to HPLC using a .Bondapak C18 col- umn eluted with 30:70 methanol-water, 0.005M in so- dium phosphate buffer, pH 8.0. In all cases, two radioactive peaks, designated al, and a2 and eluting at approximately 15 and 18 mL, respectively, were obtained, which were positive for glucuronic acid. The HPLC resolution of the a peak obtained from rat bile is shown in Figure 7. Upon mild acid hydro- lysis (pH 5) under aerobic conditions, the a, peak to K 3&I 0 0 U- 0 z P 0 49 IL 227 cc it I M. I NUSSBA UM ET AL. CH3 CH3 100, 80 60, 40, 20 I 20 40 60 80 100 120 140 160 100m 60 197 40, 1S no 220 2428 = mie m/e FIGURE 5. Mass spectra of aglycones recovered from mild acid (pH 5) hydrolysis of Sephadex LH-20 peak a. yielded DMAB as the only component (Fig. 8, upper trace) whereas hydrolysis and HPLC of a2 yielded 3,2'-dimethyl-4-nitrosobiphenyl as the major compo- nent and a small amount of DMAB (Fig. 8, lower trace). This indicates that the Sephadex LH-20 a peak was in fact composed of two glucuronic acid conjugates: the N-glucuronide of DMAB (a1) and the N-hydroxy-N-glucuronide of DMAB (a2). The approx- imate ratio of a2 to a1 was 5:1 in rat bile, 1:3 in ham- ster bile and 1:2 in hamster urine, as determined by HPLC. Sephadex LH-20 peak P was present in the urines and biles of both hamsters and rats (Figs. 2 and 3). On TLC with solvent system A, a naphthoresorcin- ol-positive, radioactive band with an Rf = 0.74 was noted in all four cases. A strong immediate reaction with Ehrlich reagent spray indicated the presence of a free amine group. Following P-glucuronidase hy- drolysis, the aglycone was recovered by ether ex- traction and purified by TLC with solvent system B (Rf = 0.46). The purified aglycone yielded a mass spectrum (Fig. 9) which is compatible with that of a 228 IN VIVO METABOLISM OF DMAB (I) >-I 0 0 n 5.0 E 6.2 c V z 2 B- 7.8 0 4 TIME, min FIGURE 6. Time course of hydrolysis of Sephadex LH-20 peak a at pH 5, pH 6.2 and pH 7.8. CH, CH, CH, =C ON CH, CH, NH, OC, 4 8 12 16 20 ELUTION VOLUME, ml FIGURE 8. HPLC of pH 5 hydrolysis products of a, and a2. A pBondapak C,8 column was eluted with 75% methanol. 100 80 60 40 20- 16 20 FIGURE 7. Resolution of Sephadex LH-20 peak a (rat bile) into glucuronides (a, and a2) by HPLC. A ,uBondapak C,, column was eluted with 30% methanol, 0.005 M sodium phosphate, pH 8.0. ring hydroxylated metabolite of DMAB. Sephadex LH-20 peak y, present in both rat urine and bile and in hamster urine was positive for glu- curonic acid. After P-glucuronidase hydrolysis of y obtained from rat urine, TLC in system B gave a major radioactive zone with an Rf of 0.27. Mass spectral analysis of the aglycone yielded distinct peaks at nme = 255 (M+), 213, 198, 181 and 163. We infer that peak y represents the glucuronide of N- acetyl ring-hydroxylated DMAB. Sephadex LH-20 peak 6, a major metabolite in rat urine and possibly a minor metabolite in rat bile and hamster urine gave an Rf of 0.60 on TLC using solvent system A. The metabolite was negative for glucuronic acid and gave a yellow color with Ehrlich reagent which developed only after a period of time. The greater elution volume of 6 compared to the glucuronides a and P on Sephadex LH-20, which 10 8l 6 04 2 CH3 CH3 OH 2 OH 20 40 60 80 100 120 140 160 10. 213 0 18 198 I6 . L0 200 . 2,0 240 2 28 0 160 ISO 200 2i 2' 26'0 2' 30 m/e FIGURE 9. Mass spectrum of the aglycone of Sephadex LH-20 peak P. A ring hydroxylated metabolite is indicated; the position of the hydroxyl group cannot be determined by mass spectral analysis alone. effects separations mainly by molecular sieving, suggested that peak 6 might be a sulfuric acid conjugate. In fact after incubation with aryl sulfatase at 370C for 4 hr at 370C, more than 80% of the total radioactivity was extractable into ether. The concentrated ether extract gave a single radioactive band on TLC with system B with an R1 of 0.27. Submission of the eluted aglycone to mass spectrometry gave a spectrum essentially identical to that obtained using the aglycone of peak y. Thus peak 6 represents the sulfate ester of N-acetyl-C- hydroxy DMAB. E c C4 z 0 0 4 4 8 12 ELUTION VOLUME, ml ., I . , ., , ,1,, . ,.1 1 1. -.I. I I L11 1. l 229 NUSSBA UM ET AL. Discussion Among the arylamine carcinogens, DMAB is of interest because it induces colon tumors in rats and hamsters (1-7, 11), mammary tumors in female rats (8) and urinary bladder tumors in hamsters (7, 11, 12). Thus, depending on the animal, DMAB provides a good experimental model for three major sites of human cancers. Since it contrasts with other colon carcinogens such as 1,2-dimethylhydrazine whose activated metabolites reach the colon mucosa via the blood rather than the fecal stream (22), as is the case with DMAB (9, 10), clarification of the mode of action of these carcinogens may yield mutually com- plementary information that can be relevant to the as yet unknown etiology of the disease in man. The distinct differences (excepting the overlap in the small and large intestines) in the organotropism of DMAB in the rat and hamster presumably result from differences in the metabolism and disposition of the carcinogen. In this respect, it is of interest that the rat, in which intestinal tumors are the ma- jor lesions, excretes a greater proportion of DMAB metabolites in the bile than in the urine (Fig. 1). In contrast, the hamster, which is sensitive to the de- velopment of urinary bladder tumors in response to DMAB, excretes a greater portion of the metabo- lites in the urine than in the bile. A still better correlation exists between the pres- ence of the metabolite which we have identified, al- beit through indirect methods, as the N-hydroxy- N-glucuronide of DMAB in the two physiological fluids and the sites of tumor formation in the two species. Thus this metabolite is present in both the bile and urine of hamsters, and is present in rat bile but not rat urine (Figs. 2 and 3). Because of its insta- bility, the amount of the N-hydroxy-N-glucuronide is difficult to quantitate accurately, however we esti- mate that 4.5% of the DMAB dose is excreted in this form in rat bile, 1.2% is excreted in hamster bile and 4.2% is excreted in hamster urine in the first 24-hr period after dosing. From the work of Radomski et al. (20, 23, 24) and Kadlubar et al. (19, 25, 26) it appears that the pres- ence of the N-hydroxy-N-glucuronide of DMAB in hamster urine could be directly related to the induc- tion of urinary bladder tumors in this species. N-Hy- droxy-N-glucuronides of arylamines such as 4-amino- biphenyl or 2-naphthylamine are regarded (26) as transport forms and proximate carcinogens which release the ultimate carcinogen, the N-hydroxy aglycone, upon mild acid (pH 5-6) conditions such as exist in the urinary bladder of some species (25), or upon hydrolysis with P-glucuronidase (19, 23). In five separate determinations, we have found that the pH of normal, 24 hr hamster urine to vary from pH 6.2 to 6.6. Under these conditions the N-glucuronides of DMAB and N-hydroxy-DMAB may be extensively hydrolyzed (Fig. 8). The absence of bladder tumors in the rat in response to DMAB is likely due to the preferential excretion of the N-hydroxy-N-glucuro- nide in the bile rather than the urine in this species. Extensive investigations (27, 28) into the relative excretion of organic anions into the urine and bile of rats have determined that a rather sharp molecular weight threshold for biliary excretion in this species exists; compounds below the molecular weight of approximately 350 are excreted almost entirely in the urine. Above 350 molecular weight the extent of biliary excretion increases steeply as a function of the molecular weight. Thus in the rat, the extents of biliary excretion of the N-hydroxy-N-glucuronide of DMAB (molecular weight 388) would be greater than the corresponding metabolite of 3-methyl-4- aminobiphenyl (molecular weight 374) which in turn would be greater than that of N-hydroxy-N- glucuronide of 4-aminobiphenyl (molecular weight 360). Interestingly, this correlates well with the carcinogenicity of the parent amines for the colon (Table 1). The excretion of the N-hydroxy-N- glucuronide of DMAB in both hamster urine and bile could be due to a higher molecular weight threshold for biliary excretion in this species. It is obvious that besides molecular weight other factors, such as perhaps the degree of N-hydroxyla- ion and the reactivity of the ultimate carcinogen, must also play a role in determining the organotrop- ism and carcinogenicity of the 4-aminobiphenyl derivatives, since the 2-methyl and the 3-methoxy substituted compounds were observed to induce tumors only in the liver and bladder of rats, respec- tively (Table 1). Hecht et al. (15) have demonstrated that DMAB is significantly more mutagenic in the Ames assay than is 2'-methyl-4-aminobiphenyl and that the same relationship holds for the correspond- ing N-oxidized derivatives. With respect to the carcinogenicity of DMAB to the colon, bacterial enzymes may play a major role in the further activation of the N-hydroxy-N-glucu- ronide. Analogous metabolites of 2-naphthylamine and AB have been found to be easily hydrolyzed to the aglycones by E. coli p-glucuronidase (19); more- over Reddy and Watanabe (8) have demonstrated that germ-free status significantly reduces the inci- dence of DMAB induced intestinal tumors in F344 rats. Up to now relatively little information has been available as to the metabolism of DMAB (5, 29). However, Gorrod (30), in unpublished work, has de- tected the N-glucuronide of DMAB in rat bile and has obtained evidence for the oxidation of both the 3 and 2' methyl groups as well as for hydroxylation 230 IN VIVO METABOLISM OF DMAB 2:1 at the 4' position. Work on the more exact function of the N-hydroxy-N-glucuronide in intestinal and uri- nary bladder cancer as well as on the complete char- acterization of the biliary and urinary DMAB me- tabolites resolved by our Sephadex LH-20 method is currently in progress in this laboratory. This study was supported in part by NIOSH Grant OH- 00611 and by NCI Grant 15400 through the National Large Bowel Cancer Project. The advice of Dr. S. Hecht in parts of this study is gratefully acknowledged. We thank Mr. Chang Choi for expertly performing the bile duct cannulations and Joseph Camanzo and Ruth Young for the mass spectral analyses. REFERENCES 1. Walpole, A. L., Williams M. H. C., and Roberts, D. C. The carcinogenic action of 4-aminodiphenyl and 3:2'-dimethyl- 4-aminodiphenyl. Brit. J. Ind. Med. 9: 255-263 (1952). 2. Walpole, A. L., and Williams, M. H. C. Aromatic amines as carcinogens in industry. Brit. Med. Bull. 14: 141-145 (1958). 3. Walpole, A. L. In: Berliner Symposium uber Fragen der Carcinogenese. Akadamie Verlag, Berlin 1960, pp. 9-11. 4. Spjut, H. J. Experimental induction of tumors of the large bowel of rats. Cancer 28: 29-37 (1970). 5. Spjut, H. J., and Noall, M. W. Colonic neoplasms induced by 3,2'-dimethyl-4-aminobiphenyl. In: Carcinoma of the Colon and Antecedent Epithelium (W. J. Burdette, Ed.), C. C. Thomas, Springfield, IL 1970, pp. 280-288. 6. Spjut, H. J., and Spratt, J. S., Jr. Endemic and morpho- logic similarities existing between spontaneous colonic neoplasms in man and 3,2'-dimethyl-4-aminobiphenyl in- duced colonic neoplasms in rats. Ann. Surg. 161: 309-324 (1965). 7. So, B. T., and Wynder, E. L. Induction of hamster tumors of the urinary bladder by 3,2'-dimethyl-4-aminobiphenyl. J. Natl. Cancer Inst. 48: 1733-1735 (1972). 8. Reddy, B. S., and Watanabe, K. Effect of intestinal micro- flora on 3,2'-dimethyl-4-aminobiphenyl-induced carcinogen- esis in F344 rats. J. Natl. Cancer Inst. 61: 1269-1271 (1978). 9. Navarette-Reyna, A., and Spjut, H. J. The effect of colos- tomy on experimentally produced neoplasms of the colon of the rat. Federation Proc. 25: 2 (1966). 10. Cleveland, J. C., Litvak, S. F., and Cole, J. W. Identifica- tion of the route of action of the carcinogen 3,2'-dimethyl- 4-aminobiphenyl in the induction of intestinal neoplasia. Cancer Res. 27: 708-714 (1967). 11. Williams, G. M., Chandrasekaran, V., Katayama, S., and Weisburger, J. H. Carcinogenicity of 3-methyl-2-naphthyl- amine and 3,2'-dimethyl-4-aminobiphenyl to the bladder and gastrointestinal tract of the Syrian golden hamster with atypical proliferative enteritis. J. Natl. Cancer Inst. 67: 481-488 (1981). 12. Fiala, E. S., Weisburger, J. H., Katayama, S., Chandrasek- aran, V., and Williams, G. M. The effect of disulfiram on the carcinogenicity of 3,2'-dimethyl-4-aminobiphenyl in Syrian golden hamsters and rats. Carcinogenesis 2: 965- 969 (1981). 13. Fiala, E. S., Nussbaum, M., and Weisburger, J. H. Biliary metabolites of 3,2'-dimethyl-4-aminobiphenyl (DMAB) in the F344 rat, Proc. Am. Assoc. Cancer Res. 21: 119 (1980). 14. Zweig, G., and Sherma, J. (Eds). CRC Handbook on Chro- matography, Vol. II, CRC Press, 1972, p. 147. 15. Hecht, S. S., El-Bayoumy, K., Tulley, L., and LaVoie, E. Structure-mutagenicity relationships of N-oxidized deriv- atives of aniline, o-toluidine, 2'-methyl-4-aminobiphenyl and 3,2'-dimethyl-4-aminobiphenyl. J. Med. Chem. 22: 981- 987 (1979). 16. Son, 0. S., Everett, D. W., and Fiala, E. S. Metabolism of o-[methyl- 4C] toluidine in the F344 rat. Xenobiotica 10: 457-468 (1980). 17. Mead, J. A. R., Smith, J. N., and Williams, R. T. The me- tabolism of hydroxycoumarins. Biochem. J. 68: 61-67 (1958). 18. Boyland, E., Manson, D., and Orr, S. F. D. The biochemis- try of aromatic amines, 2. The conversion of arylamines in arylsulphonic acids and arylamine-N-glucosiduronic acids, Biochem. J. 65: 417-423 (1957). 19. Kadlubar, F. F., Miller, J. A., and Miller, E. C. Hepatic mi- crosomal N-glucuronidation and nucleic acid binding of N- hydroxy arylamines in relation to urinary bladder carcino- genesis. Cancer Res. 37: 805-814 (1977). 20. Radomski, J. L., Rey, A. A., and Brill, E. Evidence for a glucuronic acid conjugate of N-hydroxy-4-aminobiphenyl in the urine of dogs given 4-aminobiphenyl, Cancer Res. 33: 1284-1289 (1973). 21. Irving, C. C. Conjugation of N-hydroxy compounds. In: Metabolic Conjugation and Metabolic Hydrolysis (W. H. Fishman, Ed.), Academic Press, New York, 1970, pp. 53- 119. 22. Fiala, E. S. Inhibition of carcinogen metabolism and action by disulfiram, pyrazole and related compounds. In: Inhibi- tion of Tumor Induction and Development. (M. S. Zedeck and M. Lipkin, Eds.), Plenum Press, New York, 1981, pp. 23-69. 23. Moreno, H. R., and Radomski, J. L. Synthesis of the uri- nary glucuronic acid conjugate of N-hydroxy-4-aminobi- phenyl. Cancer Letters 4: 85-88 (1978). 24. Poupko, J. M., Hearn, W. L., and Radomski, J. L. N-Glu- curonidation of N-hydroxy aromatic amines: a mechanism for their transport and bladder-specific carcinogenicity. Toxicol. Appl. Pharmacol. 50: 479-484 (1979). 25. Kadlubar, F., Flammang, T., and Unruh, L. The role of N- hydroxy arylamine N-glucuronides in arylamine-induced urinary bladder carcinogenesis: metabolite profiles in acidic, neutral and alkaline urines of 2-naphthylamine and 2-nitronaphthalene-treated rats. In: Conjugation Reac- tions in Drug Biotransformation (A. Aitio, Ed.), Elsevier/ North Holland, Amsterdam, 1978, pp. 443-454. 26. Kadlubar, F. F., Unruh, L. E., Flammang, T. J., Sparks, D., Mitchum, R. K., and Mulder, G. J. Alteration of uri- nary levels of the carcinogen N-hydroxy-2-naphthylamine and its N-glucuronide in the rat by control of urinary pH, inhibition of metabolic sulfation, and changes in biliary ex- cretion. Chem.-Biol. Interactions 33: 129-147 (1981). 27. Hirom, P. C., Millburn, P., Smith, R. L., and Williams, R. T. Species variations in the threshold molecular-weight factor for the biliary excretion of organic anions, Biochem. J. 129: 1071-1077 (1972). 28. Hirom, P. C., Millburn, P., and Smith, R. L. Bile and urine as complementary pathways for excretion of foreign or- ganic compounds. Xenobiotica 6: 55-64 (1976). 29. Koes, M. T., Bartling, G. J., Forrester, L. J., Mitra, M. N., Chattopadhyay, S. K., and Brown, H. D. Preliminary stud- ies on the metabolism of 3,2'-dimethyl-4-aminobiphenyl in the rat, Texas Repts. Biol. Med. 33: 282-292 (1975). 30. Gorrod, J. W. (Chelsea College, University of London). Unpublished work, personal communication.