i
Efficient delivery of lentiviral vectors into resting human CD4 T cells
-
Published Date:
Feb 27 2014
Source:Gene Ther. 21(4):444-449.
Details:
-
Alternative Title:Gene Ther
-
Personal Author:
-
Description:Resting human CD4 T cells are highly resistant to transfection or infection with lentiviral vectors derived from the human immunodeficiency virus. We now describe a flexible and efficient approach involving virus-like particles containing simian immunodeficiency virus lentiviral gene product protein X and pseudotyping with CXCR4-tropic HIV Env. This method permits effective genetic manipulation of these cells while preserving their naturally quiescent state. This technology can also be extended to primary lymphoid cultures where authentic cellular composition and functional relationships are preserved.
-
Subject:
-
Pubmed ID:24572792
-
Pubmed Central ID:PMC4006095
-
Document Type:
-
Funding:
-
Collection(s):
-
Main Document Checksum:
- File Type:
-
Supporting Files:
application/msword application/octet-stream image/gif image/jpeg image/gif image/jpeg
No Related Documents.