The study comprised 2 experimental parts: 1) oral infection conducted at the Friedrich-Loeffler-Institut and 2) intramuscular infection at NRIVVaMR. For oral infection, we used a 2008 isolate from Armenia. The experiment was conducted by using 6 wild boar piglets 9 weeks of age. Three domestic pigs were used as contact controls and were handled in the same manner as the wild boar piglets. The animals were kept under high-containment conditions. After acclimatization, the wild boars were infected orally with 2 mL of a spleen suspension containing 10⁶ median tissue culture infectious dose ASFV/mL. Two days after infection, 3 domestic weaner pigs were added to the pen with the wild boar piglets. Starting from the day of infection, rectal temperature and clinical signs were recorded. Oral and fecal swabs were collected from the wild boars at 0, 1, 2, 3, 5, 6, and 7 days postinfection (dpi). In addition, blood samples were taken at 0, 2, 5, 6, and 7 dpi. Blood from the domestic pigs was sampled at 0, 6, 9, and 13 dpi. Necropsy was performed on all animals.

For real-time quantitative PCR (qPCR), viral DNA was extracted by using manual and automated extraction methods according to manufacturer instructions. Subsequently, qPCR was performed according to the protocol published by King et al. (7) with slight modifications by using an Mx3005P PCR Cycler (Stratagene, La Jolla, CA, USA).

For intramuscular infection, 4 wild boars 9 months of age were brought to the containment stables of the NRIVVaMR. One animal was inoculated intramuscularly with 1,000 hemadsorbing units 50% of a 2009 virus isolate from the Chechen Republic, which is identical to the isolate used in the oral trial in all genome fragments routinely sequenced. The remaining animals were housed together with the infected animal as contact controls.

Clinical signs of infection were recorded every day. Samples of visceral organs, skin, and hair were taken during necropsy and subjected to qPCR. Isolation of viral DNA was performed by using an in-house kit based on the modified method published by Boom et al. (8). The qPCR for ASFV detection was carried out according to the protocol published by King et al. (7) with a Rotorgene 6000 instrument (Corbett Research, Sydney, Queensland, Australia).

After oral infection, an acute fatal course of the disease developed in all wild boar piglets, and they died within 7 days. Apart from severe depression, slight diarrhea, and reduced feed intake, only high fever was observed starting 3–4 dpi. During postmortem examinations, enlarged and hemorrhagic lymph nodes (Figure 1) and hemorrhagic gastritis (Figure 2) were observed. Acute fatal ASF developed in 2 of the domestic pigs 11–12 dpi of the wild boars. These animals died 1 week later, showing severe but unspecific signs. One domestic pig became infected later. It only showed fever at 20 dpi and was euthanized on day 25. Infection of this animal was clearly linked to contact with blood from a moribund pen mate.

During the clinical phase of the disease, qPCR was positive for all blood samples with first positive results 2 dpi. Oropharyngeal and fecal swabs were positive mainly on days 6 and 7. An overview of the qPCR results is presented in Table 1.

On the third day after intramuscular inoculation, the infected wild boar showed depression, inappetence, and increased respiratory frequency. It died at 5 dpi showing hemorrhagic nasal discharge. The 3 contact animals showed similar symptoms at 8 dpi of the intramuscularly infected wild boar and died 2 days later. Postmortem examinations showed hemorrhages in multiple edematously enlarged lymph nodes, most prominent pulmonary hyperemia and alveolar edema, hyperplasia of the mesenteric lymph nodes, and acute gastritis with hemorrhages. Skin lesions were not present.

ASF genome was detected in the samples of visceral organs and lymph nodes of all animals. In samples of skin and kidneys, viral DNA was detected only in the infected animal. Results of qPCR are presented in the Table 2.

Knowledge about disease dynamics in domestic pigs and wild boars is a prerequisite for risk assessment and prevention strategy design. Unfortunately, wild boar data are scarce. To contribute to this information, animal trials were conducted for an experimental characterization of recent Caucasian ASFV isolates in wild boars.

We concluded that the Caucasian isolates are highly virulent in wild boars. Both oral and intramuscular infection resulted in 100% lethality.

PCR results showed that the ASFV genome is easily detected in blood and organ samples of diseased animals. Swab samples were positive in the clinical phase of infection but showed much lower genome loads. Shedding of ASFV through nasal discharge or feces, and thus overall contagiousness, seems to be limited.

Transmission to domestic pigs was delayed in comparison to transmission to wild boars. The most likely reason for this difference seems to be contact with blood. Although this factor could be observed most certainly for the contact wild boars, domestic pigs had only limited contact with blood.

On the basis of these data, it seems unlikely the Caucasian isolates have the potential to become endemic in European wild boar populations without a distinct change in virulence. So far no indications exist that the virulence of ASFV is changing in affected regions in Russia.

A risk factor for disease control could be the involvement of tick vectors. Until now, no indications exist that ticks are involved in ASFV outbreaks in the Caucasus region and Russia. Moreover, it has to be kept in mind that the wild boar’s way of life does not facilitate contact with soft ticks. Nevertheless, this possibility was not examined during this study and needs further investigation.