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Efficient In Vivo Genome Editing Using RNA-Guided Nucleases
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3 2013
Source: Nat Biotechnol. 31(3):227-229 -
Alternative Title:Nat Biotechnol
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Description:In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.
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Pubmed ID:23360964
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Pubmed Central ID:PMC3686313
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