Targeted Deletion and Inversion of Tandemly Arrayed Genes in Arabidopsis thaliana Using Zinc Finger Nucleases

Supporting Information for Qi et al., 2013

Files in this Data Supplement:

  • Supporting Information - Figures S1-S9 and Tables S1-S2 (PDF, 1 MB)
  • Figure S1 - DNA sequences for zinc finger arrays (PDF, 297 KB)
  • Figure S2 - ZFN expression entry clone-pZHY013 (PDF, 316 KB)
  • Figure S3 - ZFNs that target the ASK8 gene cluster and a lectin RLK gene cluster (PDF, 368 KB)
  • Figure S4 - CoDA-assembled ZFNs are active in T1 plants. (PDF, 315 KB)
  • Figure S5 - At1g53-ZFN activity at two targets revealed by enrichment PCR in T2 plants (PDF, 312 KB)
  • Figure S6 - An active ADH1-ZFN #3 line (PDF, 411 KB)
  • Figure S7 - A possible NHEJ repair mechanism using 1-bp of microhomology (PDF, 365 KB)
  • Figure S8 - Inversion of the At1g70450 gene cluster (PDF, 347 KB)
  • Figure S9 - Duplication of a gene cluster or circularization of deleted DNA at the At1g70450-At1g70460 locus (PDF, 377 KB)
  • Table S1 - Zinc finger arrays, recognition sites and recognition helices (PDF, 299 KB)
  • Table S2 - Oligos for amplifying Arabidopsis DNA (PDF, 299 KB)