Immunotoxicity of In Vitro Vanadium Exposures: Effects on Interleukin-1, Tumor Necrosis Factor-A, and Prostaglandin E2 Production by WEHI-3 Macrophages

Details

• Personal Author:
  Cohen MD ; Parsons E ; Schlesinger RB ; Zelikoff JT
• Description:
  Treatment of cultured mouse macrophages with either of two different vanadium compounds was shown to affect the production/release of two major immunoregulatory cytokines. The pentavalent vanadium compound ammonium metavanadate was shown previously to disrupt cell-mediated immunity at the earliest stages of an in vivo anti-Listerial response, in that mice treated with vanadium displayed decreased accessory cell recruitment and numbers of activated macrophages at infection sites. To determine whether these effects were due to vanadium-induced alterations in the production of biologically-active mediators, mouse macrophage-like WEHI-3 cells were treated in vitro with ammonium metavanadate or vanadium pentoxide prior to stimulation with lipopolysaccharide endotoxin (LPS). After stimulation, monokine (tumor necrosis factor-a and interleukin-1) and prostaglandin E2 (PGE2) activities were assessed. Both vanadium compounds decreased recovered monokine activities; measured TNFa concentrations were also reduced. Spontaneous release of the IL-1/TNF-regulating prostanoid PGE2 was significantly increased by the highest concentration of vanadate tested, although LPS-stimulated PGE2 production was unaffected by either compound. These results indicate that, in vitro, pentavalent vanadium can interfere with immunoregulatory mediators critical for maintaining host immunocompetence. [Description provided by NIOSH]
• Subjects:
  Animals Immune System Occupational Health Safety
• Keywords:
  Animal Studies Cellular Effects Cytokines Immune Reaction Immunotoxins In Vitro Studies Vanadium Compounds
• ISSN:
  0192-0561
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  437-446
• Volume:
  15
• Issue:
  3
• NIOSHTIC Number:
  nn:20060106
• Citation:
  Int J Immunopharmacol 1993 Apr; 15(3):437-446
• Contact Point Address:
  Mitchell D. Cohen, Department of Environmental Medicine, New York University Medical Center, Long Meadow Road, Tuxedo, NY 10987, U.S.A.
• CAS Registry Number:
  Sodium vanadium oxide (Na3VO4) (CAS RN 13721-39-6)
• Federal Fiscal Year:
  1993
• Performing Organization:
  Mount Sinai School of Medicine, New York
• Peer Reviewed:
  True
• Start Date:
  19920701
• Source Full Name:
  International Journal of Immunopharmacology
• End Date:
  19970630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5ec5613e297ccb241a777022a8cc796ec405cca4fba3dd33807d309a32ef886bfed2198b632e55839de71db7be7006bede463b0c45ccf6101c815f0ab89649ee
• Download URL:
  https://stacks.cdc.gov/view/cdc/224121/cdc_224121_DS1.pdf
• File Type:
  [PDF - 807.76 KB ]