Details:

Alternative Title:
J Parasitol Res
Personal Author:
Mull, Bonnie J. ; Narayanan, Jothikumar ; Hill, Vincent R.
Mull, Bonnie J. ; Narayanan, Jothikumar ; Hill, Vincent R. Less -
Description:
Primary amebic meningoencephalitis (PAM) is a rare and typically fatal infection caused by the thermophilic free-living ameba, Naegleria fowleri. In 2010, the first confirmed case of PAM acquired in Minnesota highlighted the need for improved detection and quantification methods in order to study the changing ecology of N. fowleri and to evaluate potential risk factors for increased exposure. An immunomagnetic separation (IMS) procedure and real-time PCR TaqMan assay were developed to recover and quantify N. fowleri in water and sediment samples. When one liter of lake water was seeded with N. fowleri strain CDC:V212, the method had an average recovery of 46% and detection limit of 14 amebas per liter of water. The method was then applied to sediment and water samples with unknown N. fowleri concentrations, resulting in positive direct detections by real-time PCR in 3 out of 16 samples and confirmation of N. fowleri culture in 6 of 16 samples. This study has resulted in a new method for detection and quantification of N. fowleri in water and sediment that should be a useful tool to facilitate studies of the physical, chemical, and biological factors associated with the presence and dynamics of N. fowleri in environmental systems.
Subjects:
[+]
Research Article
Source:
J Parasitol Res. 2013; 2013.
Document Type:
Journal Article
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:07c9ca69944bfd8c9612e3b6bc007e9efd5822f9ddfbf902d4bf82aec9032a67
Download URL:
https://stacks.cdc.gov/view/cdc/21221/cdc_21221_DS1.pdf
File Type:

Supporting Files

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