An integrated electrochemical device based on immunochromatographic test strip and enzyme labels for sensitive detection of disease-related biomarkers

Details

• Personal Author:
  Li Y-Q ; Lin Y ; Wang H ; Wang J ; Zou Z-X
• Description:
  A novel electrochemical biosensing device that integrates an immunochromatographic test strip and a screen-printed electrode (SPE) connected to a portable electrochemical analyzer was presented for rapid, sensitive, and quantitative detection of disease-related biomarker in human blood samples. The principle of the sensor is based on sandwich immunoreactions between a biomarker and a pair of its antibodies on the test strip, followed by highly sensitive square-wave voltammetry (SWV) detection. Horseradish peroxidase (HRP) was used as a signal reporter for electrochemical readout. Hepatitis B surface antigen (HBsAg) was employed as a model protein biomarker to demonstrate the analytical performance of the sensor in this study. Some critical parameters governing the performance of the sensor were investigated in detail. Under optimal conditions, this sensor was capable of detecting a minimum of 0.3 ng mL(-1) (S/N=3) HBsAg with a wide linear concentration range from 1 to 500 ng mL(-1). The sensor was further utilized to detect HBsAg spiked in human plasma with an average recovery of 91.3%. In comparison, a colorimetric immunochromatographic test strip assay (ITSA) was also conducted. The result shows that the SWV detection in the electrochemical sensor is much more sensitive for the quantitative determination of HBsAg than the colorimetric detection, indicating that such a sensor is a promising platform for rapid and sensitive point-of-care testing/screening of disease-related biomarkers in a large population. [Description provided by NIOSH]
• Subjects:
  Antibodies Antigens Biochemistry Biological Assay Biomarkers Blood Chemistry Chemistry, Analytic Chromatography Communicable Diseases Hematologic Tests Immune System Occupational Health Safety

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• Keywords:
  Analytical-chemistry Analytical-instruments Antibody-response Author Keywords: Immunochromatographic Test Strip Assay Bioassays Biochemical-indicators Bioelectric-effects Blood-samples Chromatographic-analysis Detectors Disease-related Biomarker Electrochemical-analysis Electrochemical-properties Equipment-reliability Hepatitis Hepatitis B Horseradish Peroxidase Humans Immune-reaction Immune-system Immunochemistry Peroxidases Protein-biochemistry Proteins Quantitative-analysis Surface Antigen Testing-equipment

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• ISSN:
  0039-9140
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  58-64
• Volume:
  94
• NIOSHTIC Number:
  nn:20041491
• Citation:
  Talanta 2012 May; 94:58-64
• Contact Point Address:
  Yuehe Lin, Pacific Northwest National Laboratory, Richland, WA 99352, USA
• Email:
  yqlig@xmu.edu.cn
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Battelle Pacific Northwest Laboratories
• Peer Reviewed:
  True
• Start Date:
  20060901
• Source Full Name:
  Talanta
• End Date:
  20170831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c2904395ff9ea292f025891fd575f4b2f693472fd4f02ec0734af13412a1970de199b7eb8108d4edaa5b5e512cdacb02ad558967cda17775be71d45355595a31
• Download URL:
  https://stacks.cdc.gov/view/cdc/194050/cdc_194050_DS1.pdf
• File Type:
  [PDF - 702.87 KB ]