In April 2008, fecal specimens were collected from 116 macaques (3–6 years of age) with diarrhea on a monkey farm in People’s Republic of China. Feces were suspended to 10% (wt/vol) in phosphate-buffered saline (0.01 M, pH 7.4), and total RNA was extracted from 200 µL by using TRIZOL reagent (Invitrogen, Carlsbad, CA, USA). Viral RNA was dissolved in 30 µL RNase-free water and stored at –80°C.

Primers (outside-L 5′-CTAGAGAGCTTGGCCGTCGG-3′, outside-R 5′-GTACCTTCTGGGCATCCTTC-3′, inside-L 5′-GGCCTTATACCCCGACTTGC-3′, and inside-R 5′-GGCCTTACAACTAGTGTTTG-3′) (12) were used for reverse transcription nested PCR to identify diverse HPeV genotypes by amplification of a 518-bp fragment located in the 5′ untranslated region (UTR). The expected-size DNA bands were excised from an agarose gel, purified with the AxyPrep DNA gel extraction kit (Axygen, Union City, CA, USA), cloned into pMD-18T vector (TaKaRa, Dalian, China), and sequenced (Applied Biosystems 3730 DNA Analyzer; Invitrogen). Feces from 6 of 116 monkeys were positive for HPeV. The HPeV sequences were compared with those of the HPeV genotype reference strains by using BLAST (www.ncbi.nlm.nih.gov/BLAST). Five of the 6 sequences showed closest identity to the 5′ UTR of HPeV-1 (90%–94%). The viral protein (VP)3/VP1 region of these 5 viruses was then PCR amplified and sequenced to confirm type 1 identity (13). The last monkey feces–derived sequence showed 97%–98% nucleotide identity to HPeV-6 strains. This strain was fully sequenced.

A 674-nt region of the 5′ UTR, an open reading frame (ORF) encoding a polyprotein precursor of 2,182 aa, and a partial 3′ UTR of 88 nt (7,311 bp) were sequenced. The near full genome showed 96% nucleotide identity with the genotype 6 reference genome (AB252582). The polyprotein encoded capsid proteins VP0 (312 aa), VP3 (229 aa), and VP1 (234 aa) and nonstructural proteins 2A (150 aa), 2B (122 aa), 2C (329 aa), 3A (117 aa), 3B (20 aa), 3C (200 aa), and 3D (469 aa). The integrin binding motif arginine–glycine–aspartic acid was identified close to the C terminus of VP1 (8,14).

We performed phylogenetic analysis using the nearly full genome of SH6 and 17 representative HPeV and related viruses (Figure). Results confirmed that SH6 belonged to genotype 6 and clustered closely with the reference genome from Japan and a strain from the Netherlands (EU077518), forming an HPeV-6 subgroup (Figure).

Previous studies have documented frequent human infections with different HPeV genotypes (www.picornaviridae.com/parechovirus/hpev/hpev.htm). We detected HPeV genotypes 1 and 6 in the feces of 6 of 116 monkeys with diarrhea. A similar analysis of a healthy monkey control group is needed to determine whether an association exists between HPeV infections of monkeys and diarrhea. On the basis of the close similarities between virus feces–derived sequences and HPeV, these viruses might have been transmitted by the fecal–oral route from humans to monkeys. The multiple HPeV genotypes detected indicated that viral transmission occurred on multiple occasions. Monkeys may therefore serve as an animal model for HPeV infection and possibly pathogenesis.