In Georgia, during 2005–2006, residents were encouraged to submit ticks to the state’s Division of Public Health for identification and testing for various tick-borne pathogens. We studied only ticks that had been attached to persons. Ticks were individually homogenized with metal beads and resuspended in 225 μL of phosphate-buffered saline. DNA was extracted from 100 μL of the homogenate by using a QIAamp DNA Micro Kit (QIAGEN Inc, Valencia, CA, USA) according to the manufacturer's instructions. We conducted a nested PCR targeting the 17-kDa gene (7) and used bidirectional sequencing at the Integrated Biotechnology Laboratories (University of Georgia, Athens, GA, USA) to confirm positive results.

In Tennessee, from April 2007 through September 2008, the United States Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, and the Tennessee Department of Health collected A. americanum and A. maculatum ticks from 31 counties by examining wild animals and dragging flannel sheets through vegetation. Ticks were stored in 100% ethanol and were sent to the Tennessee Department of Health Vector-Borne Diseases Laboratory for identification of species and life stage and detection of pathogens by molecular testing. A PCR targeting the outer membrane protein A (rOmpA) gene of spotted fever group (SGF) rickettsiae was conducted as previously described, using primers Rr190.602n and Rr190.70p (8). To identify the rickettsiae species, we subjected positive samples to a restriction fragment length polymorphism (RFLP) assay by digestion of the amplicons with RsaI (Promega, Madison, WI, USA) and PstI (Fermentas, Glen Burnie, MD, USA) enzymes at 37°C for 2 h. Digested fragments were subjected to electrophoresis on 10% polyacrylamide gels. To confirm species identification, we purified representative positive PCR products with Exosap-It (USB Corporation, Cleveland, OH, USA), sequenced at the Tennessee Department of Health Laboratory Services (Nashville, TN, USA) and entered into the National Center for Biotechnology Information BLAST database (www.blast.ncbi.nlm.nih.gov/Blast.cgi).

Of ticks collected in Georgia, 418 A. americanum ticks (237 adults, 180 nymphs, and 1 unknown) and 19 A. maculatum ticks, were submitted for testing. Of these, 1 A. americanum tick (Fayette County, May 2005) and 1 A. maculatum tick (Morgan County, July 2005) were positive for R. parkeri (100% identity with GenBank accession no. U17008).

Of 611 A. americanum and 2 A. maculatum ticks collected in Tennessee, 446 A. americanum (164 adults and 282 nymphs) and 2 A. maculatum adults were individually tested for Rickettsia spp. An additional 103 A. americanum larvae were divided in 10 pools of 4–19 ticks each according to collection site. A single A. americanum adult male (0.2% of total) had a positive RFLP pattern that matched the previously described rOmpA gene pattern of R. parkeri (8). The sequence of this amplicon (532 bp, GenBank accession no. FJ793521) was 99% similar to that of R. parkeri (EU715288). The positive tick had been collected from a coyote in Knox County, Tennessee, in July 2007.

We identified R. parkeri in ticks in Tennessee and Georgia. In Tennessee, our identification of only 2 A. maculatum ticks (the primary vector of R. parkeri) supports previous reports that this tick species is uncommon in Tennessee (9). In Georgia, the 19 A. maculatum ticks identified were <5% of the total number of ticks collected in Georgia. In contrast to these low numbers, A. americanum ticks are ubiquitous in high densities throughout the other southeastern states and readily feed on humans (8). Recently, the range of A. americanum ticks has expanded in the United States and now extends from west-central Texas to the Atlantic Coast, encompassing the entire Southeast and parts of the lower Midwest and coastal New England (10). The distribution of A. americanum ticks completely overlaps the suspected distribution of A. maculatum ticks (Figure). The positive A. americanum tick from Tennessee was collected from a free-ranging coyote, and the detected R. parkeri may have been present in the blood meal taken by the tick. However, A. americanum ticks can maintain and transmit R. parkeri infection both transovarially and transtadially (6). R. parkeri has previously been identified in A. maculatum ticks from Georgia (4), but the identification of R. parkeri in A. americanum ticks renews concerns that this tick species may be involved in the natural history of another zoonotic pathogen. Additional study is needed to determine the extent of the role of A. americanum ticks as a natural vector for R. parkeri.

This study raises concerns about the serologic diagnosis of RMSF. R. parkeri may be the etiologic agent of some rickettsiosis cases in Tennessee and Georgia that have been misdiagnosed as RMSF. Because reliable clinical tests specific for different SFG rickettsiae are not readily available, several different rickettsioses may be serologically cross-reactive, leading to misdiagnosis of RMSF (11–14). Reliable diagnosis requires PCR or culture of biopsy specimens from eschars, when present (15). Additional studies characterizing SFG rickettsioses, including development of rickettsial species–specific clinical tests, will assist in attributing rickettsiosis to R. rickettsii, R. parkeri, or other SFG rickettsial infections.