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Reproducibility of Serologic Assays for Influenza Virus A (H5N1)

Published Date:

Aug 2009

Source:

Emerg Infect Dis. 15(8):1250-1259.

[PDF-580.30 KB]

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Alternative Title:

Emerg Infect Dis

Personal Author:

Stephenson, Iain ; Heath, Alan ; Major, Diane ; Newman, Robert W. ; Hoschler, Katja ; ... More ▼

Description:

Hemagglutination-inhibition (HI) and neutralization are used to evaluate vaccines against influenza virus A (H5N1); however, poor standardization leads to interlaboratory variation of results. A candidate antibody standard (07/150) was prepared from pooled plasma of persons given clade 1 A/Vietnam/1194/2004 vaccine. To test human and sheep antiserum, 15 laboratories used HI and neutralization and reassortant A/Vietnam/1194/2004, A/turkey/Turkey/1/2005 (clade 2.2), and A/Anhui/1/2005 (clade 2.3.4) viruses. Interlaboratory variation was observed for both assays, but when titers were expressed relative to 07/150, overall percentage geometric coefficient of variation for A/Vietnam/1194/2004 was reduced from 125% to 61% for HI and from 183% to 81% for neutralization. Lack of reduced variability to clade 2 antigens suggested the need for clade-specific standards. Sheep antiserum as a standard did not reliably reduce variability. The World Health Organization has established 07/150 as an international standard for antibody to clade 1 subtype H5 and has an assigned potency of 1,000 IU/ampoule.

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Document Type:

Journal Article

Collection(s):

Emerging Infectious Diseases

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	08-1754_finalR.nxml	text/plain
	license.txt	text/plain
	08-1754-F1.gif	image/gif
	08-1754-F1.jpg	image/jpeg
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	08-1754-F3.jpg	image/jpeg
	08-1754_appT1-s1.pdf	application/pdf
	08-1754_appT2-s2.pdf	application/pdf

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Reproducibility of Serologic Assays for Influenza Virus A (H5N1)

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