Brain, tonsil, spleen, and selected lymph nodes, including RPLNs, prescapular, submandibular, superficial cervical, mesenteric, popliteal, and ileocecal-colic lymph nodes, were obtained from 10 CWD-infected elk and 15 CWD-infected deer (12 mule deer and 3 white-tailed deer). Elk were derived from game farms or research facilities where they became infected by contact with CWD-infected elk, a contaminated environment, or oral inoculation. All of the elk used in this study had definite clinical cases when they were euthanized. The deer used for PrPres quantification all had confirmed clinical cases and were from research facilities where they became infected by contact with infected animals. Three of the mule deer included in Table 1 were harvested by Colorado Division of Wildlife or Wyoming Department of Game and Fish personnel. Tissues from wild uninfected deer and elk were obtained from Montana Department of Fish, Wildlife and Parks. More than 4,000 wild deer and elk from Montana have been tested for CWD with no positives found.

Twenty-percent tissue homogenates of brain, tonsil, lymph nodes, or spleen from CWD-infected and uninfected deer and elk were made in 0.01 mmol/L Tris-HCl, pH 7.4, 0.005 mmol/L MgCl₂ by using either disposable Konex microcentrifuge tubes (Kimble/Kontes, Vineland, NJ, USA) and matched pestles (brain) or an omni tissue homogenizer (tonsil, spleen, and lymph nodes); 75%–90% of the total tissue mass of respective lymph nodes or tonsil was homogenized. Two-hundred–milligram aliquots of the total homogenate were processed further to concentrate PrPres by using ultracentifugation and proteinase K digestion as described (20).

Protein gel electrophoresis and immunoblotting were done as previously described (21,22) by using polyclonal antibody R35 (23) or monoclonal antibody L-42 (R-Biopharm AG, Darmstadt, Germany). L-42 reacts with PrPres from several species, including deer and elk, and has been well characterized (24). Blots were developed by using either an enhanced chemiluminescence (ECL) or enhanced chemifluorescence (ECF) system, according to the manufacturer’s instructions (Amersham-Pharmacia, Piscataway, NJ, USA). ECL blots were exposed to film to visualize proteins. ECF blots were scanned by using a STORM fluorescent detection system (Amersham-Pharmacia) as described previously (25).

Reagents and enzymes for PNGaseF treatment were purchased from New England BioLabs (Beverly, MA, USA). Reaction conditions were as recommended by the manufacturer except that denaturing of 1- to 30-mg tissue equivalents was done in a total volume of 20 μL sodium dodecyl sulfate–polyacrylamide gel electrophoresis sample buffer. Each sample was digested by using 2,500 U PNGaseF and incubated overnight at 37°C. Samples were frozen at −20°C until they were analyzed by immunoblotting.

Tissues from 10 CWD-affected elk and 15 CWD-affected deer were analyzed in this study. All 10 of the elk had advanced clinical CWD when euthanized. The deer represented various stages of clinical disease. Detailed data showing PrPres glycoform profiles and quantification of PrPres are shown for 5 of the elk and 6 of the deer (Figure 1). Each of the elk brains gave a very strong PrPres signal when 2-mg brain equivalents were analyzed. Deer brain PrPres was more variable, and 20 mg of brain equivalent was analyzed (Figure 1). The relative amount of PrPres in each sample was determined by comparing the PrPres signal to a standard control. The standard control for each blot was RPLN from one of the CWD-infected mule deer in the study (labeled C in each blot, Figure 1). Relative PrPres amounts were determined by using a phosphor-imager and Image Quant software (Storm, Molecular Dynamics, Sunnyvale, CA, USA). The average amount of PrPres in elk brain was consistently higher than the amount in deer brain (Figure 2). The lower amounts of PrPres in deer brain than in elk brain likely reflects the more variable and earlier clinical status of the deer that were analyzed.

Diagnosis of CWD is often based on detection of PrPres in tonsil tissue by using ELISA and IHC analysis. Therefore, we also analyzed tonsil tissue, but because we were interested in quantitative issues we used immunoblot technology.

No PrPres was observed in tonsil from 2 of the elk (Figure 1B), and only a small amount was detected in tonsil from the other 3 elk (Figures 1B, 2B). PrPres in the 3 tonsil specimens that did give a signal averaged 4% of the control’s signal. In contrast, tonsil from 5 of the 6 CWD-affected deer gave a strong PrPres signal (Figure 1B), averaging 109% of the reference control’s signal. However, the tonsil of the remaining deer (#4, Figures 1B, 2B) gave no PrPres signal on immunoblot.

PrPres quantities in RPLNs from elk were also low. RPLN from 1 elk was negative (#4, Figure 1C), while weak reactions were seen for RPLNs from the other 4 elk at 2%, 2%, 5.1%, and 13% of the control, respectively (Figures 1C, 2C). RPLNs from the deer were much stronger. RPLNs from all 6 deer were positive and ranged from 3.4% to 100% of that of the control (Figures 1C, 2C). One of the deer (#4, Figure 1B, C) had no PrPres detected in tonsil and very little in RPLN, even though the reaction from the brain of this deer was strong. Tonsils and RPLNs from 5 additional CWD-infected elk and 9 additional CWD-infected deer, including 3 white-tailed deer, showed PrPres in amounts similar to those of most elk and deer shown in Figures 1 and 2, but detailed quantification was not carried out on these samples. The combined data for all elk and deer show tonsil and RPLN specimens to be consistently PrPres positive by immunoblot in deer but positive less frequently in elk (Table 1).

We also sought to determine whether all of the nodes from individual deer contain similar levels of PrPres. Considerable variation was observed. In some deer, every node that was tested was PrPres positive, but more frequently only 1 or 2 nodes were positive. Furthermore, the intensity of the PrPres signals varied from node to node. In most deer, RPLN gave the strongest PrPres signal, but in other deer the prescapular or submandibular nodes were best (Figure 3). The mesenteric node was often positive, but generally gave a weak PrPres signal (Figure 3, lane 7). Thus, analysis of a single lymph node other than the RPLN by immunoblot would likely result in some CWD-positive deer being undetected.

The spleen has been shown to influence disease pathogenesis in both sheep and mouse models of TSE disease (7,12,13,26). Therefore, we also sought to quantify the amount of PrPres in elk and deer spleen. However, all of the animals gave very weak or no PrPres signals in spleen (Table 1) (blots not shown). Thus CWD-affected elk and deer differed from scrapie-affected sheep, in which the spleen routinely gives a strong PrPres signal.

PrPres glycoform patterns have been used to define TSE strains and have been studied extensively in deer and elk brain (23). Therefore, we evaluated the PrPres glycoform patterns of lymphoid tissues of CWD-infected deer and elk to identify profiles that might differentiate deer from elk. The glycoform profile in deer tonsil and lymph node were similar to that of the profile in deer brain (Figure 4). Likewise, there was no convincing difference in the pattern of PrPres found in deer and elk brains (Figure 1). A meaningful comparison of glycoform patterns between elk and deer tonsil and lymph nodes was not possible because none of the elk lymphoid organs gave a sufficiently strong PrPres signal.

Because PrPres band differences can be due both to differing glycosylation and different sites of proteinase cleavage, we treated samples of various tissues with PNGaseF to remove carbohydrates and thus show any differences due to proteolytic cleavages. Such differences in the PrPres structural core might provide evidence for the existence of different CWD strains as seen before in other TSE diseases (27–29). However, no differences were detected in PNGaseF-digested PrPres from elk and deer brain (Figure 4). Thus, both glycoform profiles and PNGaseF analysis indicated that PrPres from elk and deer were similar.