Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Blanco, D. R. ; Miller, J. N. ; Lovett, M. A.
Blanco, D. R. ; Miller, J. N. ; Lovett, M. A. Less -
Description:
A unique physical feature of Treponema pallidum, the venereally transmitted agent of human syphilis, is that its outer membrane contains 100-fold less membrane-spanning protein than the outer membranes of typical gram-negative bacteria, a property that has been related to the chronicity of syphilitic infection. These membrane-spanning T. pallidum rare outer membrane proteins, termed TROMPs, represent potential surface-exposed virulence determinants and targets of host immunity. Only recently has the outer membrane of T. pallidum been isolated and its constituent proteins identified. Five proteins of molecular mass 17-, 28-, 31-, 45-, and 65-kDa were outer membrane associated. The 17- and 45-kDa proteins, which are also present in greater amounts with the T. pallidum inner membrane protoplasmic cylinder complex, had been previously characterized lipoproteins and are, therefore, not membrane-spanning but rather membrane-anchored by their lipid moiety. In contrast, the 28-, 31-, and 65-kDa proteins are exclusively associated with the outer membrane. Both the purified native and an Escherichia coli recombinant outer membrane form of the 31-kDa protein, designated Tromp1, exhibit porin activity, thereby confirming the membrane-spanning outer membrane topology of Tromp1. The 28-kDa protein, designated Tromp2, has sequence characteristics in common with membrane-spanning outer membrane proteins and has also been recombinantly expressed in E. coli, where it targets exclusively to the E. coli outer membrane. The 65-kDa protein, designated Tromp3, is present in the least amount relative to Tromps1 and 2. Tromps 1, 2, and 3 were antigenic when tested with serum from infection and immune syphilitic rabbits and humans. These newly identified TROMPs provide a molecular foundation for the future study of syphilis pathogenesis and immunity.
Subjects:
[+]
Animals Antigens, Bacterial Antigens, Surface Bacterial Outer Membrane Proteins Bacterial Proteins Escherichia Coli Freeze Fracturing Humans Membrane Proteins Microscopy, Electron Porins Rabbits Recombinant Proteins Syphilis Treponema Pallidum Virulence
Source:
Emerg Infect Dis. 3(1):11-20.
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:07216cc21030cea956fb790422c2b441451ba3ec2d5f8f49bf9381774cc9e189
Download URL:
https://stacks.cdc.gov/view/cdc/15371/cdc_15371_DS1.pdf
File Type:

Supporting Files

• 	9126440.nxml	txt
  	license.txt	txt

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