Emerg Infect DiseidEmerging Infectious Diseases1080-60401080-6059Centers for Disease Control109983852627960Research ArticlePrevalence of non-O157:H7 shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska.FeyP. D.WickertR. S.RuppM. E.SafranekT. J.HinrichsS. H.University of Nebraska Medical Center, Omaha, Nebraska 68198-5400, USA.Sep-Oct200065530533

We determined the prevalence of Shiga toxin-producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT- SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen (4.2%) of 335 specimens were positive by at least one method (CT-SMAC culture [6 of 14], EHEC enzyme immunoassay [13 of 14], stx1,2 PCR [14 of 14]). Six contained serogroup O157, while non-O157 were as prevalent as O157 serogroups.