Evaluation of a Latex Agglutination Assay for the Identification of Burkholderia pseudomallei and Burkholderia mallei

Duval, Brea D.; Elrod, Mindy G.; Gee, Jay E.; Chantratita, Narisara; Tandhavanant, Sarunporn; Limmathurotsakul, Direk; Hoffmaster, Alex R.

doi:10.4269/ajtmh.14-0025

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Evaluation of a Latex Agglutination Assay for the Identification of Burkholderia pseudomallei and Burkholderia mallei

Apr 07 2014
By Duval, Brea D. ; Elrod, Mindy G. ; Gee, Jay E. ; ...
http://dx.doi.org/10.4269/ajtmh.14-0025
Source: Am J Trop Med Hyg. 2014; 90(6):1043-1046.

[PDF-456.32 KB]

English

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Alternative Title:

Am J Trop Med Hyg
Personal Author:

Duval, Brea D. ; Elrod, Mindy G. ; Gee, Jay E. ; Chantratita, Narisara ; Tandhavanant, Sarunporn ; ... More +

Duval, Brea D. ; Elrod, Mindy G. ; Gee, Jay E. ; Chantratita, Narisara ; Tandhavanant, Sarunporn ; Limmathurotsakul, Direk ; Hoffmaster, Alex R. Less -
Description:

Cases of melioidosis and glanders are rare in the United States, but the etiologic agents of each disease (Burkholderia pseudomallei and Burkholderia mallei, respectively) are classified as Tier 1 select agents because of concerns about their potential use as bioterrorism agents. A rapid, highly sensitive, and portable assay for clinical laboratories and field use is required. Our laboratory has further evaluated a latex agglutination assay for its ability to identify B. pseudomallei and B. mallei isolates. This assay uses a monoclonal antibody that specifically recognizes the capsular polysaccharide produced by B. pseudomallei and B. mallei, but is absent in closely related Burkholderia species. A total of 110 B. pseudomallei and B. mallei were tested, and 36 closely related Burkholderia species. The latex agglutination assay was positive for 109 of 110 (99.1% sensitivity) B. pseudomallei and B. mallei isolates tested.
Subjects:

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Antibodies, Monoclonal Burkholderia Mallei Burkholderia Pseudomallei Glanders Latex Fixation Tests Melioidosis Polysaccharides, Bacterial Species Specificity Time Factors
Source:

Am J Trop Med Hyg. 2014; 90(6):1043-1046.
Document Type:

Journal Article
Volume:

90
Issue:

6
Collection(s):

CDC Public Access
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urn:sha256:2dd53f10c23114dc0dda1f60321823ec888f7b2559525ad0095ec01084d79427
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https://stacks.cdc.gov/view/cdc/27280/cdc_27280_DS1.pdf
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	license.txt	txt
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