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Enterovirus D68 (EV-D68) 2014 outbreak strain-specific real-time reverse transcription / Polymerase chain reaction (rRT-PCR) assay instructions-Version 10/14/2014
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10/16/14
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Alternative Title:Polymerase chain reaction (rRT-PCR) assay instructions-Version 10/14/2014
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Description:The CDC Picornavirus Laboratory from mid-September to mid-October developed and evaluated the EV-D68-specific rRT-PCR assay. The assay and protocol are primarily focused on evaluating respiratory disease due to EV-D68. Some developmental and validation information can be found in Appendix B. The protocol that follows has worked reliably in our hands with little difficulty in any phase of the testing process, including interpretation of results.
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