Keratinocytes and melanocytes have distinct and shared responses to metal-based nanocatalysts and UVB.

Details

• Personal Author:
  Guppi S ; Kagan VE ; Kisin ER ; Shrivastava IH ; Shvedova AA
• Description:
  Metal-based nanocatalysts (NCT) have an irreplaceable role in energy conversion, chemical production, and automotive exhaust purification. Common metal NCT include transition metals (Me) and alloys, which provide active sites for catalytic reactions. However, the same properties that make these NCT very attractive can pose potential health risks. In addition, interactions of nanoparticles (NPs) with constituent skin cell types, in particular after the cells have been subjected to environmental stress like UVB exposure (considered as the main cause of skin cancer) are essential. In this study, we evaluated the ability of four different NCT (NiFe2O4, CoFe2O4', Ni and Co3O4') to initiate oxidative stress, induce redox-sensitive transcription factors and trigger inflammatory response in primary human epidermal keratinocytes (HEK) and melanocytes (HEM). Me/MeO-exposed cells, both HEK and HEM, revealed a dose- and time-dependent reduction in viability, cell damage, activation of NF-kB, elevated ROS generation, release of inflammatory mediators, increase in oxidative stress and DNA damage markers. Me/MeO adverse responses were significantly amplified by pre-exposure of both cell types to UVB (4KJ/m2). Hierarchical clustering analysis of inflammatory responses suggested cell-type specific effects as well as treatment related differences. Interestingly, only HEM-exposure to Me/MeO resulted in the amplification of placenta growth factors (VEGF) response (with and without pre-exposure to UVB). Notably, VEGF plays a crucial role in melanoma invasiveness, vasculogenic mimicry and tumor-associated angiogenesis. HEK pre-exposure to UVB induced expression of IL-17, a pro-inflammatory cytokine, associated with hyperproliferation and abnormal differentiation of keratinocytes. Our results indicate that topically applied metal NP may exert differential cellular effects on UVB exposed skin which are important in the context of neoplastic transformations. [Description provided by NIOSH]
• Subjects:
  Environmental Exposure Metals Neoplasms Occupational Health Risk Assessment Risk Factors Safety Skin Skin Diseases
• Keywords:
  Nanotechnology; Metal compounds; Metallic compounds; Metals; Alloys; Exposure levels; Risk factors; Health effects; Nanoparticles; Skin; Cell function; Environmental stress; Skin cancer; Oxidative stress
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  186
• NIOSHTIC Number:
  nn:20064936
• Citation:
  Toxicologist 2022 Mar; 186(S1):221
• CAS Registry Number:
  Cobalt oxide (CoO) (CAS RN 1307-96-6) ; Nickel (CAS RN 7440-02-0)
• Federal Fiscal Year:
  2022
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 61st Annual Meeting & ToxExpo, March 27-31, 2022, San Diego, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b7dfb4a74662eb34cd07dc1b3e05d0f4e5f84deccf6b7a923fd08268d182ce3dceecc43135c0c5ebbe86c6ddfb9ddcaa9f32b0df405db7b4711ca0de172fefea
• Download URL:
  https://stacks.cdc.gov/view/cdc/248691/cdc_248691_DS1.pdf
• File Type:
  [PDF - 242.10 KB ]