An improved ultrasensitive enzyme-linked immunosorbent assay using hydrangea-like antibody-enzyme-inorganic three-in-one nanocomposites

Details

• Personal Author:
  Dai Z ; Du D ; Lin Y ; Wei T ; Zhu M-J
• Description:
  Protein-inorganic nanoflowers, composed of protein and copper(II) phosphate (Cu3(PO4)2), have recently grabbed people's attention. Because the synthetic method requires no organic solvent and because of the distinct hierarchical nanostructure, protein-inorganic nanoflowers display enhanced catalytic activity and stability and would be a promising tool in biocatalytical processes and biological and biomedical fields. In this work, we first coimmobilized the enzyme, antibody, and Cu3(PO4)2 into a three-in-one hybrid protein-inorganic nanoflower to enable it to possess dual functions: (1) the antibody portion retains the ability to specifically capture the corresponding antigen; (2) the nanoflower has enhanced enzymatic activity and stability to produce an amplified signal. The prepared antibody-enzyme-inorganic nanoflower was first applied in an enzyme-linked immunosorbent assay to serve as a novel enzyme-labeled antibody for Escherichia coli O157:H7 (E. coli O157:H7) determination. The detection limit is 60 CFU L(-1), which is far superior to commercial ELISA systems. The three-in-one antibody (anti-E. coli O157:H7 antibody)-enzyme (horseradish peroxidase)-inorganic (Cu3(PO4)2) nanoflower has some advantages over commercial enzyme-antibody conjugates. First, it is much easier to prepare and does not need any complex covalent modification. Second, it has fairly high capture capability and catalytic activity because it is presented as aggregates of abundant antibodies and enzymes. Third, it has enhanced enzymatic stability compared to the free form of enzyme due to the unique hierarchical nanostructure. [Description provided by NIOSH]
• Subjects:
  Antibodies Antigens Enzymes Occupational Health Phosphoric Acids Safety
• Keywords:
  Analytical Instruments Author Keywords: ELISA Bacteria Catalysts Copper Compounds Detectors E. Coli O157:H7 ELISA Enzyme-labeled Antibody Enzyme-linked Immunosorbent Assay Enzyme Activity Inorganic Compounds Nanocomposites Nanoflowers Nanomaterials Nanostructures Nanotechnology Phosphates Protein-inorganic Nanoflower Proteins Sensitivity Testing Synthetics Ultrasensitive

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• ISSN:
  1944-8244
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  8
• Issue:
  10
• NIOSHTIC Number:
  nn:20049055
• Citation:
  ACS Appl Mater Interfaces 2016 Mar; 8(10):6329-6335
• Contact Point Address:
  Zhihui Dai, Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, P. R. China
• Email:
  daizhihuii@njnu.edu.cn
• CAS Registry Number:
  Cupric phosphate (CAS RN 7798-23-4)
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Washington State University
• Peer Reviewed:
  True
• Start Date:
  20150901
• Source Full Name:
  ACS Applied Materials and Interfaces
• End Date:
  20180831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6c231c10de4353f5281e7c7b39e912826c4b3fd248188d03c1fd81206b02a1b85ee495fd34aa7714f2a32ae22465afae04682f9f26fec3eb3b020a22f9634493
• Download URL:
  https://stacks.cdc.gov/view/cdc/203635/cdc_203635_DS1.pdf
• File Type:
  [PDF - 5.88 MB ]