PbCrO4 Mediates Cellular Responses via Reactive Oxygen Species

Details

• Personal Author:
  Antonini JM ; Castranova, Vincent ; Leonard, Stephen S. ; Roberts, Jennifer R. ; Shi X
• Description:
  Exposure to certain particulate hexavalent chromium [Cr(VI)] compounds, such as lead chromate (PbCrO4), has been associated with lung cancer and respiratory tract toxicity· Previous studies indicate that the solubility of Cr(VI)-compounds is an important factor in Cr(VI)-induced carcinogenesis· The present study investigates reactive oxygen species (ROS) generation by PbCrO4 particles and cellular responses using RAW 264·7 cells· A mixture containing PbCrO4 and RAW 264·7 cells generated hydroxyl radical (·OH), using cellularly generated H2O2 as a precursor, as measured by electron spin resonance (ESR) spin trapping in combination with H2O2 and ·OH scavengers, catalase and sodium formate· The effect of ascorbic acid on ·OH radicals was also measured using ESR· Confocal microscopy showed that particles could become either bound to the cell surface or engulfed over a 120 min time period· H2O2 generation and O2 consumption were also increased after treatment of the cells with PbCrO4· Both NF-?B and AP-1 were activated after exposure to PbCrO4 particles as measured by the NF-?B or AP-1 luciferase reporter plasmid assay· Our investigation thus demonstrated that the RAW 264·7 cells phagocytized the PbCrO4 particles leading to accumulation of the particles within vacuoles in the cytoplasm· These particles could induce chronic production of ROS and activation of NF-?B and AP-1· Such induction of transcription pathways may be involved in the inflammatory and carcinogenic responses induced by Cr(VI)-containing particles. [Description provided by NIOSH]
• Subjects:
  Carcinogens Environmental Monitoring Irritants Lead Lung Lung Diseases Metals Neoplasms Occupational Health Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  AP-1 Author Keywords: PbCrO4 Electron Spin Resonance (ESR) Exposure-assessment Exposure-limits Hydroxyl Radicals Lead-compounds Lung-cancer Lung-disease Metal-compounds NF-kB Pulmonary-system-disorders RAW 264.7 Cells Respiratory-irritants Respiratory-system-disorders

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• ISSN:
  0300-8177
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  171-179
• Volume:
  255
• Issue:
  1
• NIOSHTIC Number:
  nn:20024209
• Citation:
  Mol Cell Biochem 2004 Jan; 255(1-2):171-179
• Contact Point Address:
  Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV; Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown, WV
• Email:
  xshi@cdc.gov
• CAS Registry Number:
  Chromium(6+) (CAS RN 18540-29-9) ; Lead chromate (PbCrO4) (CAS RN 7758-97-6)
• Federal Fiscal Year:
  2004
• Peer Reviewed:
  True
• Source Full Name:
  Molecular and Cellular Biochemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:828c86de050bd02431ba262c44456d3137546e695669a753048a15a67453dc98b0c9653d97802a2cb1f4449d743b8114ac1bacd48a063377995529e48c5f8de8
• Download URL:
  https://stacks.cdc.gov/view/cdc/196140/cdc_196140_DS1.pdf
• File Type:
  [PDF - 176.21 KB ]