IL-6 deficiency exacerbates skin inflammation in a murine model of irritant dermatitis

Details

• Personal Author:
  Gallucci RM ; Lee EG ; Mickle-Kawar BM
• Description:
  Contact dermatitis is the second most reported occupational injury associated with workers compensation. Inflammatory cytokines are closely involved with the development of dermatitis, and their modulation could exacerbate skin damage, thus contributing to increased irritancy. IL-6 is a pro-inflammatory cytokine paradoxically associated with both skin healing and inflammation. To determine what role this pleiotropic cytokine plays in chemically-induced irritant dermatitis, IL-6 deficient (KO), IL-6 over-expressing transgenic (TgIL6), and corresponding wild-type (WT) mice were exposed to acetone or the irritants JP-8 jet fuel or benzalkonium chloride (BKC) daily for 7 days. Histological analysis of exposed skin was performed, as was tissue mRNA and protein expression patterns of inflammatory cytokines via QPCR and multiplex ELISA. The results indicated that, following JP-8 exposure, IL-6KO mice had greatly increased skin IL-1β, TNFa, CCL2, CCL3, and CXCL1 mRNA and corresponding product protein expression when compared to that of samples from WT counterparts and acetone-exposed control mice. BKC treatment induced the expression of all cytokines examined as compared to acetone, with CCL2 significantly higher in skin from IL-6KO mice. Histological analysis showed that IL-6KO mice displayed significantly more inflammatory cell infiltration as compared to WT and TgIL6 mice in response to jet fuel. Analysis of mRNA for the M2 macrophage marker CD206 indicated a 4-fold decrease in skin of IL-6KO mice treated with either irritant as compared to WT. Taken together, these observations suggest that IL-6 acts in an anti-inflammatory manner during irritant dermatitis, and these effects are dependent on the chemical nature of the irritant. [Description provided by NIOSH]
• Subjects:
  Acetone Aircraft Animals Dermatitis Energy Metabolism Genetics Hydrocarbons Irritants Laboratories Men Occupational Health Safety Skin Skin Diseases Women

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• Keywords:
  Acetones Author Keywords: IL-6 Cytokines Humans Inflammation Injuries Jet-engine-fuels Laboratory-animals Metabolism Skin-disorders Workers

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• ISSN:
  1547-691X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Oklahoma ; OSHA Region 6
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  192-200
• Volume:
  10
• Issue:
  2
• NIOSHTIC Number:
  nn:20043542
• Citation:
  J Immunotoxicol 2013 Apr-Jun; 10(2):192-200
• Contact Point Address:
  Randle M. Gallucci, PhD, OUHSC College of Pharmacy, PO Box 26901, Oklahoma City, OK 73126-0901
• Email:
  randy-gallucci@ouhsc.edu
• CAS Registry Number:
  Acetone (CAS RN 67-64-1) ; Quaternary ammonium compounds, alkylbenzyldimethyl, chlorides (CAS RN 8001-54-5)
• Federal Fiscal Year:
  2013
• Performing Organization:
  University of Oklahoma Health Sciences Center
• Peer Reviewed:
  True
• Start Date:
  20090701
• Source Full Name:
  Journal of Immunotoxicology
• End Date:
  20110630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d68955b2858bb7f1e0cfd7728722ff4ddeb5e80faece4051d71ac7849a8740186718f942ef3e4734238f00a32e1476b4ab30eee42a933b75961f5e556887e0f8
• Download URL:
  https://stacks.cdc.gov/view/cdc/195420/cdc_195420_DS1.pdf
• File Type:
  [PDF - 1.03 MB ]