Paraoxon Reversibly Inhibits Neurotoxic Esterase

Details

• Personal Author:
  Abou-Donia MB ; Carrington CD
• Description:
  It has recently been reported that two paraoxon-insensitive carboxylesterases may be distinguished by their sensitivity to mipafox. However, we have not been able to reliably detect two components under the conditions of the widely used assay for neurotoxic esterase (NTE). We have determined that this discrepancy is a result of differences in the technique of preinhibition by paraoxon and mipafox. We report here that paraoxon is apparently able to reduce the rate of inhibition of both neurotoxic esterase isozymes by mipafox in a concentration-dependent manner. As a result, the rate of inhibition of NTE by mipafox is greater when sequential, rather than concurrent, preinhibition is utilized. An apparently greater reduction in the inhibition rate of the more sensitive minor component may make the two isozyme species indistinguishable in the presence of paraoxon at concentrations at or above 40 µ M. [Description provided by NIOSH]
• Subjects:
  Environmental Monitoring Nervous System Occupational Health Safety
• Keywords:
  Central-nervous-system Exposure-assessment Exposure-levels Neurotoxic-effects Neurotoxicity
• ISSN:
  0041-008X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  175-178
• Volume:
  79
• Issue:
  1
• NIOSHTIC Number:
  nn:20037448
• Citation:
  Toxicol Appl Pharmacol 1985 Jun; 79(1):175-178
• Federal Fiscal Year:
  1985
• NORA Priority Area:
  Neurotoxic Effects
• Performing Organization:
  Duke University, Durham, North Carolina
• Peer Reviewed:
  True
• Start Date:
  19840601
• Source Full Name:
  Toxicology and Applied Pharmacology
• End Date:
  19881031
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d3fbbe1c480b5c3fdfc23e2d0c6c5578af6155258b77a57e76acf39a07a1a7db391af7666fa8daa97afc2ce5e3d5896e6db6ea73953bf249620d40a7e8a03ecd
• Download URL:
  https://stacks.cdc.gov/view/cdc/188543/cdc_188543_DS1.pdf
• File Type:
  [PDF - 345.55 KB ]