Mediator(s) from human monocytes/macrophages induced by quartz dust DQ12 or coal mine dust TF-1 are leading to release of oxygen radicals from human granulocytes.

Details

• Personal Author:
  Behrendt H ; Maly E ; Maly FE ; Manojlovic N ; Seemayer NH
• Description:
  The activation of the oxidative burst of polymorphonuclear granulocytes (PMN) by supernatants of quartz (14808607) or coal mine dust exposed cultures of human mononuclear cells were investigated. The morphological changes of PMN under influence of quartz dust induced mononuclear cell supernatants with a transmission electron microscope were also evaluated. Quartz dust DQ 12 was used as fibrogenic stimulus for cultures of human mononuclear cells. The results indicated that human monocytes/macrophage in culture release a soluble mediator following incubation with quartz dust DQ 12 or coal mine dust TF-1. The mediator(s) stimulates human PMN to the release of reactive oxygen species, especially of superoxide anion. This mediator was identified as granulocyte activating mediator (GRAM). The relative thermoresistance of GRAM and the sensitivity of GRAM against treatment with protease or chymotrypsin suggested a protein nature of GRAM. Besides the enhancement of the oxidative burst of PMN by GRAM, the induction of strong chemotactic changes was also noted along with the release of lysosomal products form GRAM treated PMN in a time dependent manner by ultrastructural analysis. The authors state that these results present an in-vitro example of possible nondirect mechanisms of quartz and coal mine dust pathogenicity.
• Subjects:
  Accidents, Occupational Air Pollutants, Occupational Carcinogens Health Personnel Occupational Health Personal Protective Equipment Safety Urogenital Diseases Urogenital System
• Keywords:
  Cell Cultures Cell Damage Coal Dust Cytotoxic Effects In Vitro Study Mining Industry Respiratory System Disorders
• Publisher:
  National Institute for Occupational Safety and Health
• Document Type:
  Text
• Genre:
  Conference/Symposia Proceedings
• Place as Subject:
  OSHA Region 3 ; Pennsylvania
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1466-1469
• NIOSHTIC Number:
  nn:00198710
• Citation:
  Proceedings of the VIIth International Pneumoconioses Conference, August 23-26, 1988, Pittsburgh, Pennsylvania, USA. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, National Institute for Occupational Safety and Health, DHHS (NIOSH) Publication No. 90-108, 1990 Nov; (Part II):1466-1469
• CAS Registry Number:
  Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  1991
• Peer Reviewed:
  False
• Part Number:
  II
• Source Full Name:
  Proceedings of the VIIth International Pneumoconioses Conference, August 23-26, 1988, Pittsburgh, Pennsylvania, USA
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9f3aa2bc69fa3effbdb294f8d7c23df930fd7d4c5ed5199505d36ca08e6b32506649572406cd30e6588f9942bac945876c3ebb2e31557b9d4fbb99869e1b2c88
• Download URL:
  https://stacks.cdc.gov/view/cdc/180623/cdc_180623_DS1.pdf
• File Type:
  [PDF - 174.41 KB ]